Novel Antioxidant Collagen Peptides of Siberian Sturgeon (<i>Acipenser</i><i>baerii</i>) Cartilages: The Preparation, Characterization, and Cytoprotection of H<sub>2</sub>O<sub>2</sub>-Damaged Human Umbilical Vein Endothelial Cells (HUVECs)
For making full use of aquatic by-products to produce high value-added products, Siberian sturgeon (<i>Acipenser baerii</i>) cartilages were degreased, mineralized, and separately hydrolyzed by five kinds of proteases. The collagen hydrolysate (SCH) generated by Alcalase showed the stron...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/5/325 |
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| author | Yan Sheng Yi-Ting Qiu Yu-Mei Wang Chang-Feng Chi Bin Wang |
| author_facet | Yan Sheng Yi-Ting Qiu Yu-Mei Wang Chang-Feng Chi Bin Wang |
| author_sort | Yan Sheng |
| collection | DOAJ |
| description | For making full use of aquatic by-products to produce high value-added products, Siberian sturgeon (<i>Acipenser baerii</i>) cartilages were degreased, mineralized, and separately hydrolyzed by five kinds of proteases. The collagen hydrolysate (SCH) generated by Alcalase showed the strongest 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) and hydroxide radical (HO·) scavenging activity. Subsequently, thirteen antioxidant peptides (SCP1-SCP3) were isolated from SCH, and they were identified as GPTGED, GEPGEQ, GPEGPAG, VPPQD, GLEDHA, GDRGAEG, PRGFRGPV, GEYGFE, GFIGFNG, PSVSLT, IELFPGLP, LRGEAGL, and RGEPGL with molecular weights of 574.55, 615.60, 583.60, 554.60, 640.64, 660.64, 885.04, 700.70, 710.79, 602.67, 942.12, 714.82, and 627.70 Da, respectively. GEYGFE, PSVSLT, and IELFPGLP showed the highest scavenging activity on DPPH· (EC<sub>50</sub>: 1.27, 1.05, and 1.38 mg/mL, respectively) and HO· (EC<sub>50</sub>: 1.16, 0.97, and 1.63 mg/mL, respectively), inhibiting capability of lipid peroxidation, and protective functions on H<sub>2</sub>O<sub>2</sub>-damaged plasmid DNA. More importantly, GEYGFE, PSVSLT, and IELFPGLP displayed significant cytoprotection on HUVECs against H<sub>2</sub>O<sub>2</sub> injury by regulating the endogenous antioxidant enzymes of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) to decrease the contents of reactive oxygen species (ROS) and malondialdehyde (MDA). Therefore, the research provided better technical assistance for a higher-value utilization of Siberian sturgeon cartilages and the thirteen isolated peptides—especially GEYGFE, PSVSLT, and IELFPGLP—which may serve as antioxidant additives for generating health-prone products to treat chronic diseases caused by oxidative stress. |
| first_indexed | 2024-03-10T03:31:27Z |
| format | Article |
| id | doaj.art-010810a4f6284707a6ad75df0f4e7991 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T03:31:27Z |
| publishDate | 2022-05-01 |
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| series | Marine Drugs |
| spelling | doaj.art-010810a4f6284707a6ad75df0f4e79912023-11-23T11:54:37ZengMDPI AGMarine Drugs1660-33972022-05-0120532510.3390/md20050325Novel Antioxidant Collagen Peptides of Siberian Sturgeon (<i>Acipenser</i><i>baerii</i>) Cartilages: The Preparation, Characterization, and Cytoprotection of H<sub>2</sub>O<sub>2</sub>-Damaged Human Umbilical Vein Endothelial Cells (HUVECs)Yan Sheng0Yi-Ting Qiu1Yu-Mei Wang2Chang-Feng Chi3Bin Wang4Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaFor making full use of aquatic by-products to produce high value-added products, Siberian sturgeon (<i>Acipenser baerii</i>) cartilages were degreased, mineralized, and separately hydrolyzed by five kinds of proteases. The collagen hydrolysate (SCH) generated by Alcalase showed the strongest 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) and hydroxide radical (HO·) scavenging activity. Subsequently, thirteen antioxidant peptides (SCP1-SCP3) were isolated from SCH, and they were identified as GPTGED, GEPGEQ, GPEGPAG, VPPQD, GLEDHA, GDRGAEG, PRGFRGPV, GEYGFE, GFIGFNG, PSVSLT, IELFPGLP, LRGEAGL, and RGEPGL with molecular weights of 574.55, 615.60, 583.60, 554.60, 640.64, 660.64, 885.04, 700.70, 710.79, 602.67, 942.12, 714.82, and 627.70 Da, respectively. GEYGFE, PSVSLT, and IELFPGLP showed the highest scavenging activity on DPPH· (EC<sub>50</sub>: 1.27, 1.05, and 1.38 mg/mL, respectively) and HO· (EC<sub>50</sub>: 1.16, 0.97, and 1.63 mg/mL, respectively), inhibiting capability of lipid peroxidation, and protective functions on H<sub>2</sub>O<sub>2</sub>-damaged plasmid DNA. More importantly, GEYGFE, PSVSLT, and IELFPGLP displayed significant cytoprotection on HUVECs against H<sub>2</sub>O<sub>2</sub> injury by regulating the endogenous antioxidant enzymes of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) to decrease the contents of reactive oxygen species (ROS) and malondialdehyde (MDA). Therefore, the research provided better technical assistance for a higher-value utilization of Siberian sturgeon cartilages and the thirteen isolated peptides—especially GEYGFE, PSVSLT, and IELFPGLP—which may serve as antioxidant additives for generating health-prone products to treat chronic diseases caused by oxidative stress.https://www.mdpi.com/1660-3397/20/5/325Siberian sturgeon (<i>Acipenser baerii</i>)cartilagecollagen peptideantioxidant activitycytoprotection |
| spellingShingle | Yan Sheng Yi-Ting Qiu Yu-Mei Wang Chang-Feng Chi Bin Wang Novel Antioxidant Collagen Peptides of Siberian Sturgeon (<i>Acipenser</i><i>baerii</i>) Cartilages: The Preparation, Characterization, and Cytoprotection of H<sub>2</sub>O<sub>2</sub>-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) Marine Drugs Siberian sturgeon (<i>Acipenser baerii</i>) cartilage collagen peptide antioxidant activity cytoprotection |
| title | Novel Antioxidant Collagen Peptides of Siberian Sturgeon (<i>Acipenser</i><i>baerii</i>) Cartilages: The Preparation, Characterization, and Cytoprotection of H<sub>2</sub>O<sub>2</sub>-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
| title_full | Novel Antioxidant Collagen Peptides of Siberian Sturgeon (<i>Acipenser</i><i>baerii</i>) Cartilages: The Preparation, Characterization, and Cytoprotection of H<sub>2</sub>O<sub>2</sub>-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
| title_fullStr | Novel Antioxidant Collagen Peptides of Siberian Sturgeon (<i>Acipenser</i><i>baerii</i>) Cartilages: The Preparation, Characterization, and Cytoprotection of H<sub>2</sub>O<sub>2</sub>-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
| title_full_unstemmed | Novel Antioxidant Collagen Peptides of Siberian Sturgeon (<i>Acipenser</i><i>baerii</i>) Cartilages: The Preparation, Characterization, and Cytoprotection of H<sub>2</sub>O<sub>2</sub>-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
| title_short | Novel Antioxidant Collagen Peptides of Siberian Sturgeon (<i>Acipenser</i><i>baerii</i>) Cartilages: The Preparation, Characterization, and Cytoprotection of H<sub>2</sub>O<sub>2</sub>-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
| title_sort | novel antioxidant collagen peptides of siberian sturgeon i acipenser i i baerii i cartilages the preparation characterization and cytoprotection of h sub 2 sub o sub 2 sub damaged human umbilical vein endothelial cells huvecs |
| topic | Siberian sturgeon (<i>Acipenser baerii</i>) cartilage collagen peptide antioxidant activity cytoprotection |
| url | https://www.mdpi.com/1660-3397/20/5/325 |
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