Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndrome
Sjögren syndrome (SjS) is an autoimmune disease characterized by the destruction of the exocrine gland epithelia, causing a dryness of mucosa called sicca symptoms, and whose main life-threatening complication is lymphoma. There is a need for new biomarkers in this disease, notably diagnostic biomar...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1207545/full |
| _version_ | 1797778214734528512 |
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| author | Juliette Ferrant Juliette Ferrant Adeline Pontis François Zimmermann Florent Dingli Patrick Poullet Damarys Loew Karin Tarte Karin Tarte Erwan Dumontet Erwan Dumontet |
| author_facet | Juliette Ferrant Juliette Ferrant Adeline Pontis François Zimmermann Florent Dingli Patrick Poullet Damarys Loew Karin Tarte Karin Tarte Erwan Dumontet Erwan Dumontet |
| author_sort | Juliette Ferrant |
| collection | DOAJ |
| description | Sjögren syndrome (SjS) is an autoimmune disease characterized by the destruction of the exocrine gland epithelia, causing a dryness of mucosa called sicca symptoms, and whose main life-threatening complication is lymphoma. There is a need for new biomarkers in this disease, notably diagnostic biomarkers for patients with genuine sicca symptoms that do not meet current criteria, and prognostic biomarkers for patients at risk of lymphoma. Plasma extracellular vesicles (EVs) are promising biomarker candidates in several diseases, but their potential has not yet been explored in SjS. In this proof-of-concept study, we characterized EVs from primary SjS patients (pSS, n=12) at the phenotypic and proteomic levels, compared to EVs from healthy donor (HD, n=8) and systemic lupus erythematosus patients (SLE, n=12). Specific plasma EVs subpopulations, derived from neutrophils, endothelial, and epithelial cells, were found increased in pSS. We also identified a pSS proteomic signature in plasma EVs, including neutrophil-, epithelial-, and endothelial-related proteins, such as integrin alpha M (ITGAM), olfactomedin-4 (OLFM4), Ras-related protein RAB10, and CD36. Overall, our results support the relevance of plasma EVs as biomarkers in SjS. |
| first_indexed | 2024-03-12T23:14:04Z |
| format | Article |
| id | doaj.art-0109fbbb0b9f4d79aa01bc46a6c5ed94 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T23:14:04Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-0109fbbb0b9f4d79aa01bc46a6c5ed942023-07-17T12:53:24ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-07-011410.3389/fimmu.2023.12075451207545Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndromeJuliette Ferrant0Juliette Ferrant1Adeline Pontis2François Zimmermann3Florent Dingli4Patrick Poullet5Damarys Loew6Karin Tarte7Karin Tarte8Erwan Dumontet9Erwan Dumontet10Pôle Biologie, Centre Hospitalier Universitaire de Rennes, Rennes, FranceUMR, Université Rennes, INSERM, Établissement Français du Sang, Rennes, FrancePôle Biologie, Centre Hospitalier Universitaire de Rennes, Rennes, FranceDépartement de Médecine Interne et Immunologie Clinique, Centre Hospitalier Universitaire de Rennes, Rennes, FranceInstitut Curie, PSL Research University, CurieCoreTech Mass Spectrometry Proteomics, Paris, FranceInstitut Curie, PSL Research University, INSERM, Mines Paris Tech, Bioinformatics core facility (CUBIC), Paris, FranceInstitut Curie, PSL Research University, CurieCoreTech Mass Spectrometry Proteomics, Paris, FrancePôle Biologie, Centre Hospitalier Universitaire de Rennes, Rennes, FranceUMR, Université Rennes, INSERM, Établissement Français du Sang, Rennes, FrancePôle Biologie, Centre Hospitalier Universitaire de Rennes, Rennes, FranceUMR, Université Rennes, INSERM, Établissement Français du Sang, Rennes, FranceSjögren syndrome (SjS) is an autoimmune disease characterized by the destruction of the exocrine gland epithelia, causing a dryness of mucosa called sicca symptoms, and whose main life-threatening complication is lymphoma. There is a need for new biomarkers in this disease, notably diagnostic biomarkers for patients with genuine sicca symptoms that do not meet current criteria, and prognostic biomarkers for patients at risk of lymphoma. Plasma extracellular vesicles (EVs) are promising biomarker candidates in several diseases, but their potential has not yet been explored in SjS. In this proof-of-concept study, we characterized EVs from primary SjS patients (pSS, n=12) at the phenotypic and proteomic levels, compared to EVs from healthy donor (HD, n=8) and systemic lupus erythematosus patients (SLE, n=12). Specific plasma EVs subpopulations, derived from neutrophils, endothelial, and epithelial cells, were found increased in pSS. We also identified a pSS proteomic signature in plasma EVs, including neutrophil-, epithelial-, and endothelial-related proteins, such as integrin alpha M (ITGAM), olfactomedin-4 (OLFM4), Ras-related protein RAB10, and CD36. Overall, our results support the relevance of plasma EVs as biomarkers in SjS.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1207545/fullSjögren syndromeextracellular vesiclesbiomarkerproteomicsystemic lupus erythematosus |
| spellingShingle | Juliette Ferrant Juliette Ferrant Adeline Pontis François Zimmermann Florent Dingli Patrick Poullet Damarys Loew Karin Tarte Karin Tarte Erwan Dumontet Erwan Dumontet Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndrome Frontiers in Immunology Sjögren syndrome extracellular vesicles biomarker proteomic systemic lupus erythematosus |
| title | Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndrome |
| title_full | Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndrome |
| title_fullStr | Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndrome |
| title_full_unstemmed | Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndrome |
| title_short | Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndrome |
| title_sort | phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary sjogren syndrome |
| topic | Sjögren syndrome extracellular vesicles biomarker proteomic systemic lupus erythematosus |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1207545/full |
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