Dietary Supplementation with <i>Enterococcus faecium</i> R1 Attenuates Intestinal and Liver Injury in Piglets Challenged by Lipopolysaccharide
In this study, a strain of <i>E. faecium</i> R1 with effective bacteriostatic activity, acid resistance, bile salt resistance, high-temperature resistance was screened. To study the effect of <i>E. faecium</i> R1 on lipopolysaccharide (LPS)-induced intestinal and liver injury...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/5/1424 |
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| author | Yanyan Zhang Tao Wu Zhenqiang Chen Yuxuan Meng Zhekun Zhu Qian Wang Junjie Tian Dan Yi Lei Wang Di Zhao Yongqing Hou |
| author_facet | Yanyan Zhang Tao Wu Zhenqiang Chen Yuxuan Meng Zhekun Zhu Qian Wang Junjie Tian Dan Yi Lei Wang Di Zhao Yongqing Hou |
| author_sort | Yanyan Zhang |
| collection | DOAJ |
| description | In this study, a strain of <i>E. faecium</i> R1 with effective bacteriostatic activity, acid resistance, bile salt resistance, high-temperature resistance was screened. To study the effect of <i>E. faecium</i> R1 on lipopolysaccharide (LPS)-induced intestinal and liver injury in piglets, twenty-four weaned female piglets were randomly assigned into one of three groups (8 piglets per group). Piglets in the control group and LPS group were fed a basal diet, piglets in the <i>E. faecium</i> group were fed the basal diet supplemented with <i>E. faecium</i> R1 (6.5 × 10<sup>6</sup> CFU/g). On day 21 of the trial, piglets in the LPS group and <i>E. faecium</i> group were intraperitoneally administered LPS (100 μg/kg), piglets in the control group were administered the same volume of saline. Subsequently, blood samples were collected at 3 h, and intestinal, liver, and pancreas samples were collected at 6 h. Results showed that <i>E. faecium</i> R1 supplementation significantly decreased the diarrhea rate and feed to gain ratio, and dramatically reduced LPS-induced intestinal and liver injury in piglets. Compared with the LPS group, <i>E. faecium</i> R1 supplementation significantly increased the content of glucagon in plasma and IL-1β in the liver, and the mRNA levels of <i>villin</i> in jejunum and ileum and <i>Bcl-xL</i> and <i>pBD-L</i> in the ileum, and significantly decreased the contents of prostaglandin 2 and malondialdehyde in the liver and the activities of myeloperoxidase and aspartate aminotransferase in plasma in piglets. Moreover, <i>E. faecium</i> R1 improved the pancreatic antioxidant capacity in piglets, which was indicated by a significant increase in catalase activity and a decrease in total nitric oxide synthase activity. In summary, dietary supplementation with <i>E. faecium</i> R1 alleviates intestinal and liver injury in LPS-challenged piglets. |
| first_indexed | 2024-03-10T11:21:34Z |
| format | Article |
| id | doaj.art-010a7fd8418841e2a602147429947049 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T11:21:34Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-010a7fd8418841e2a6021474299470492023-11-21T19:58:06ZengMDPI AGAnimals2076-26152021-05-01115142410.3390/ani11051424Dietary Supplementation with <i>Enterococcus faecium</i> R1 Attenuates Intestinal and Liver Injury in Piglets Challenged by LipopolysaccharideYanyan Zhang0Tao Wu1Zhenqiang Chen2Yuxuan Meng3Zhekun Zhu4Qian Wang5Junjie Tian6Dan Yi7Lei Wang8Di Zhao9Yongqing Hou10Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaIn this study, a strain of <i>E. faecium</i> R1 with effective bacteriostatic activity, acid resistance, bile salt resistance, high-temperature resistance was screened. To study the effect of <i>E. faecium</i> R1 on lipopolysaccharide (LPS)-induced intestinal and liver injury in piglets, twenty-four weaned female piglets were randomly assigned into one of three groups (8 piglets per group). Piglets in the control group and LPS group were fed a basal diet, piglets in the <i>E. faecium</i> group were fed the basal diet supplemented with <i>E. faecium</i> R1 (6.5 × 10<sup>6</sup> CFU/g). On day 21 of the trial, piglets in the LPS group and <i>E. faecium</i> group were intraperitoneally administered LPS (100 μg/kg), piglets in the control group were administered the same volume of saline. Subsequently, blood samples were collected at 3 h, and intestinal, liver, and pancreas samples were collected at 6 h. Results showed that <i>E. faecium</i> R1 supplementation significantly decreased the diarrhea rate and feed to gain ratio, and dramatically reduced LPS-induced intestinal and liver injury in piglets. Compared with the LPS group, <i>E. faecium</i> R1 supplementation significantly increased the content of glucagon in plasma and IL-1β in the liver, and the mRNA levels of <i>villin</i> in jejunum and ileum and <i>Bcl-xL</i> and <i>pBD-L</i> in the ileum, and significantly decreased the contents of prostaglandin 2 and malondialdehyde in the liver and the activities of myeloperoxidase and aspartate aminotransferase in plasma in piglets. Moreover, <i>E. faecium</i> R1 improved the pancreatic antioxidant capacity in piglets, which was indicated by a significant increase in catalase activity and a decrease in total nitric oxide synthase activity. In summary, dietary supplementation with <i>E. faecium</i> R1 alleviates intestinal and liver injury in LPS-challenged piglets.https://www.mdpi.com/2076-2615/11/5/1424weaned pigletgrowth performance<i>E. faecium</i> R1lipopolysaccharideintestineliver |
| spellingShingle | Yanyan Zhang Tao Wu Zhenqiang Chen Yuxuan Meng Zhekun Zhu Qian Wang Junjie Tian Dan Yi Lei Wang Di Zhao Yongqing Hou Dietary Supplementation with <i>Enterococcus faecium</i> R1 Attenuates Intestinal and Liver Injury in Piglets Challenged by Lipopolysaccharide Animals weaned piglet growth performance <i>E. faecium</i> R1 lipopolysaccharide intestine liver |
| title | Dietary Supplementation with <i>Enterococcus faecium</i> R1 Attenuates Intestinal and Liver Injury in Piglets Challenged by Lipopolysaccharide |
| title_full | Dietary Supplementation with <i>Enterococcus faecium</i> R1 Attenuates Intestinal and Liver Injury in Piglets Challenged by Lipopolysaccharide |
| title_fullStr | Dietary Supplementation with <i>Enterococcus faecium</i> R1 Attenuates Intestinal and Liver Injury in Piglets Challenged by Lipopolysaccharide |
| title_full_unstemmed | Dietary Supplementation with <i>Enterococcus faecium</i> R1 Attenuates Intestinal and Liver Injury in Piglets Challenged by Lipopolysaccharide |
| title_short | Dietary Supplementation with <i>Enterococcus faecium</i> R1 Attenuates Intestinal and Liver Injury in Piglets Challenged by Lipopolysaccharide |
| title_sort | dietary supplementation with i enterococcus faecium i r1 attenuates intestinal and liver injury in piglets challenged by lipopolysaccharide |
| topic | weaned piglet growth performance <i>E. faecium</i> R1 lipopolysaccharide intestine liver |
| url | https://www.mdpi.com/2076-2615/11/5/1424 |
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