Microbiome diversity in African American, European American, and Egyptian colorectal cancer patients
Purpose: Although there is an established role for microbiome dysbiosis in the pathobiology of colorectal cancer (CRC), CRC patients of various race/ethnicities demonstrate distinct clinical behaviors. Thus, we investigated microbiome dysbiosis in Egyptian, African American (AA), and European Americ...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402305243X |
| _version_ | 1797771405846118400 |
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| author | Amr Elkholy Nagavardhini Avuthu Mohammed Abdalla Michael Behring Prachi Bajpai Hyung-Gyoon Kim Doaa Header Reham AH. Abo Elwafa Hesham Saed Amira Embaby Nefertiti El-Nikhely Sarah Obuya Mostafa Mohamed Ahmed Ashour Badawy Ahmed Nawar Farrukh Afaq Laura Q. Rogers Sejong Bae James M. Shikany Lori Brand Bateman Mona Fouad Mansoor Saleh Temesgen Samuel Sooryanarayana Varambally Chittibabu Guda Waleed Arafat Upender Manne |
| author_facet | Amr Elkholy Nagavardhini Avuthu Mohammed Abdalla Michael Behring Prachi Bajpai Hyung-Gyoon Kim Doaa Header Reham AH. Abo Elwafa Hesham Saed Amira Embaby Nefertiti El-Nikhely Sarah Obuya Mostafa Mohamed Ahmed Ashour Badawy Ahmed Nawar Farrukh Afaq Laura Q. Rogers Sejong Bae James M. Shikany Lori Brand Bateman Mona Fouad Mansoor Saleh Temesgen Samuel Sooryanarayana Varambally Chittibabu Guda Waleed Arafat Upender Manne |
| author_sort | Amr Elkholy |
| collection | DOAJ |
| description | Purpose: Although there is an established role for microbiome dysbiosis in the pathobiology of colorectal cancer (CRC), CRC patients of various race/ethnicities demonstrate distinct clinical behaviors. Thus, we investigated microbiome dysbiosis in Egyptian, African American (AA), and European American (EA) CRC patients. Patients and methods: CRCs and their corresponding normal tissues from Egyptian (n = 17) patients of the Alexandria University Hospital, Egypt, and tissues from AA (n = 18) and EA (n = 19) patients at the University of Alabama at Birmingham were collected. DNA was isolated from frozen tissues, and the microbiome composition was analyzed by 16S rRNA sequencing. Differential microbial abundance, diversity, and metabolic pathways were identified using linear discriminant analysis (LDA) effect size analyses. Additionally, we compared these profiles with our previously published microbiome data derived from Kenyan CRC patients. Results: Differential microbiome analysis of CRCs across all racial/ethnic groups showed dysbiosis. There were high abundances of Herbaspirillum and Staphylococcus in CRCs of Egyptians, Leptotrichia in CRCs of AAs, Flexspiria and Streptococcus in CRCs of EAs, and Akkermansia muciniphila and Prevotella nigrescens in CRCs of Kenyans (LDA score >4, adj. p-value <0.05). Functional analyses showed distinct microbial metabolic pathways in CRCs compared to normal tissues within the racial/ethnic groups. Egyptian CRCs, compared to normal tissues, showed lower l-methionine biosynthesis and higher galactose degradation pathways. Conclusions: Our findings showed altered mucosa-associated microbiome profiles of CRCs and their metabolic pathways across racial/ethnic groups. These findings provide a basis for future studies to link racial/ethnic microbiome differences with distinct clinical behaviors in CRC. |
| first_indexed | 2024-03-12T21:37:05Z |
| format | Article |
| id | doaj.art-010f8a89f7864569b6c37779a74d7287 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T21:37:05Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-010f8a89f7864569b6c37779a74d72872023-07-27T05:58:19ZengElsevierHeliyon2405-84402023-07-0197e18035Microbiome diversity in African American, European American, and Egyptian colorectal cancer patientsAmr Elkholy0Nagavardhini Avuthu1Mohammed Abdalla2Michael Behring3Prachi Bajpai4Hyung-Gyoon Kim5Doaa Header6Reham AH. Abo Elwafa7Hesham Saed8Amira Embaby9Nefertiti El-Nikhely10Sarah Obuya11Mostafa Mohamed12Ahmed Ashour Badawy13Ahmed Nawar14Farrukh Afaq15Laura Q. Rogers16Sejong Bae17James M. Shikany18Lori Brand Bateman19Mona Fouad20Mansoor Saleh21Temesgen Samuel22Sooryanarayana Varambally23Chittibabu Guda24Waleed Arafat25Upender Manne26Department of Pathology, University of Alabama at Birmingham, AL, USA; Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, EgyptDepartment of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USADepartment of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, EgyptDepartment of Pathology, University of Alabama at Birmingham, AL, USADepartment of Pathology, University of Alabama at Birmingham, AL, USADepartment of Pathology, University of Alabama at Birmingham, AL, USADepartment of Gastroenterology, Faculty of Medicine, University of Alexandria, EgyptDepartment of Clinical Pathology, Faculty of Medicine, University of Alexandria, EgyptDepartment of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, EgyptDepartment of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, EgyptDepartment of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, EgyptMoi Teaching and Referral Hospital, Moi University, Kesses, KenyaDepartment of Pathology, University of Alabama at Birmingham, AL, USA; Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, EgyptDepartment of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, University of Alexandria, EgyptDepartment of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, University of Alexandria, EgyptDepartment of Pathology, University of Alabama at Birmingham, AL, USADivision of Preventive Medicine, University of Alabama at Birmingham, AL, USA; O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USADivision of Preventive Medicine, University of Alabama at Birmingham, AL, USA; O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USADivision of Preventive Medicine, University of Alabama at Birmingham, AL, USA; O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USADivision of Preventive Medicine, University of Alabama at Birmingham, AL, USA; O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USADivision of Preventive Medicine, University of Alabama at Birmingham, AL, USA; O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USADepartment of Hematology-Oncology, Aga Khan University, Nairobi, KenyaTuskegee University College of Veterinary Medicine Tuskegee, AL, USADepartment of Pathology, University of Alabama at Birmingham, AL, USA; O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USADepartment of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USADepartment of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, University of Alexandria, Egypt; Corresponding author. Clinical Oncology, Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Alexandria University, Alexandria 21131, Egypt.Department of Pathology, University of Alabama at Birmingham, AL, USA; O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA; Corresponding author. Director of Translational Anatomic Pathology, Wallace Tumor Institute, Room # 420A, University of Alabama at Birmingham, Birmingham, AL 35233, USA.Purpose: Although there is an established role for microbiome dysbiosis in the pathobiology of colorectal cancer (CRC), CRC patients of various race/ethnicities demonstrate distinct clinical behaviors. Thus, we investigated microbiome dysbiosis in Egyptian, African American (AA), and European American (EA) CRC patients. Patients and methods: CRCs and their corresponding normal tissues from Egyptian (n = 17) patients of the Alexandria University Hospital, Egypt, and tissues from AA (n = 18) and EA (n = 19) patients at the University of Alabama at Birmingham were collected. DNA was isolated from frozen tissues, and the microbiome composition was analyzed by 16S rRNA sequencing. Differential microbial abundance, diversity, and metabolic pathways were identified using linear discriminant analysis (LDA) effect size analyses. Additionally, we compared these profiles with our previously published microbiome data derived from Kenyan CRC patients. Results: Differential microbiome analysis of CRCs across all racial/ethnic groups showed dysbiosis. There were high abundances of Herbaspirillum and Staphylococcus in CRCs of Egyptians, Leptotrichia in CRCs of AAs, Flexspiria and Streptococcus in CRCs of EAs, and Akkermansia muciniphila and Prevotella nigrescens in CRCs of Kenyans (LDA score >4, adj. p-value <0.05). Functional analyses showed distinct microbial metabolic pathways in CRCs compared to normal tissues within the racial/ethnic groups. Egyptian CRCs, compared to normal tissues, showed lower l-methionine biosynthesis and higher galactose degradation pathways. Conclusions: Our findings showed altered mucosa-associated microbiome profiles of CRCs and their metabolic pathways across racial/ethnic groups. These findings provide a basis for future studies to link racial/ethnic microbiome differences with distinct clinical behaviors in CRC.http://www.sciencedirect.com/science/article/pii/S240584402305243XColorectal cancerMicrobiomeAfrican AmericanEuropean AmericanEgyptian |
| spellingShingle | Amr Elkholy Nagavardhini Avuthu Mohammed Abdalla Michael Behring Prachi Bajpai Hyung-Gyoon Kim Doaa Header Reham AH. Abo Elwafa Hesham Saed Amira Embaby Nefertiti El-Nikhely Sarah Obuya Mostafa Mohamed Ahmed Ashour Badawy Ahmed Nawar Farrukh Afaq Laura Q. Rogers Sejong Bae James M. Shikany Lori Brand Bateman Mona Fouad Mansoor Saleh Temesgen Samuel Sooryanarayana Varambally Chittibabu Guda Waleed Arafat Upender Manne Microbiome diversity in African American, European American, and Egyptian colorectal cancer patients Heliyon Colorectal cancer Microbiome African American European American Egyptian |
| title | Microbiome diversity in African American, European American, and Egyptian colorectal cancer patients |
| title_full | Microbiome diversity in African American, European American, and Egyptian colorectal cancer patients |
| title_fullStr | Microbiome diversity in African American, European American, and Egyptian colorectal cancer patients |
| title_full_unstemmed | Microbiome diversity in African American, European American, and Egyptian colorectal cancer patients |
| title_short | Microbiome diversity in African American, European American, and Egyptian colorectal cancer patients |
| title_sort | microbiome diversity in african american european american and egyptian colorectal cancer patients |
| topic | Colorectal cancer Microbiome African American European American Egyptian |
| url | http://www.sciencedirect.com/science/article/pii/S240584402305243X |
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