Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a Time
Over the last few decades, in vitro single-molecule manipulation techniques have enabled the use of force and displacement as controlled variables in biochemistry. Measuring the effect of mechanical force on the real-time kinetics of a biological process gives us access to the rates, equilibrium con...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Biophysica |
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| Online Access: | https://www.mdpi.com/2673-4125/2/4/47 |
| _version_ | 1797461189283807232 |
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| author | Rebeca Bocanegra María Ortiz-Rodríguez Ismael Plaza Garcia-Abadillo Carlos R-Pulido Borja Ibarra |
| author_facet | Rebeca Bocanegra María Ortiz-Rodríguez Ismael Plaza Garcia-Abadillo Carlos R-Pulido Borja Ibarra |
| author_sort | Rebeca Bocanegra |
| collection | DOAJ |
| description | Over the last few decades, in vitro single-molecule manipulation techniques have enabled the use of force and displacement as controlled variables in biochemistry. Measuring the effect of mechanical force on the real-time kinetics of a biological process gives us access to the rates, equilibrium constants and free-energy landscapes of the mechanical steps of the reaction; this information is not accessible by ensemble assays. Optical tweezers are the current method of choice in single-molecule manipulation due to their versatility, high force and spatial and temporal resolutions. The aim of this review is to describe the contributions of our lab in the single-molecule manipulation field. We present here several optical tweezers assays refined in our laboratory to probe the dynamics and mechano-chemical properties of biological molecular motors and synthetic molecular devices at the single-molecule level. |
| first_indexed | 2024-03-09T17:15:52Z |
| format | Article |
| id | doaj.art-01120da666ec4d3ebfbe8d24ce77725b |
| institution | Directory Open Access Journal |
| issn | 2673-4125 |
| language | English |
| last_indexed | 2024-03-09T17:15:52Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biophysica |
| spelling | doaj.art-01120da666ec4d3ebfbe8d24ce77725b2023-11-24T13:35:46ZengMDPI AGBiophysica2673-41252022-12-012456458010.3390/biophysica2040047Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a TimeRebeca Bocanegra0María Ortiz-Rodríguez1Ismael Plaza Garcia-Abadillo2Carlos R-Pulido3Borja Ibarra4IMDEA Nanociencia, Campus Cantoblanco, Faraday 9, 28049 Madrid, SpainIMDEA Nanociencia, Campus Cantoblanco, Faraday 9, 28049 Madrid, SpainIMDEA Nanociencia, Campus Cantoblanco, Faraday 9, 28049 Madrid, SpainIMDEA Nanociencia, Campus Cantoblanco, Faraday 9, 28049 Madrid, SpainIMDEA Nanociencia, Campus Cantoblanco, Faraday 9, 28049 Madrid, SpainOver the last few decades, in vitro single-molecule manipulation techniques have enabled the use of force and displacement as controlled variables in biochemistry. Measuring the effect of mechanical force on the real-time kinetics of a biological process gives us access to the rates, equilibrium constants and free-energy landscapes of the mechanical steps of the reaction; this information is not accessible by ensemble assays. Optical tweezers are the current method of choice in single-molecule manipulation due to their versatility, high force and spatial and temporal resolutions. The aim of this review is to describe the contributions of our lab in the single-molecule manipulation field. We present here several optical tweezers assays refined in our laboratory to probe the dynamics and mechano-chemical properties of biological molecular motors and synthetic molecular devices at the single-molecule level.https://www.mdpi.com/2673-4125/2/4/47optical tweezerssingle-molecule manipulationDNA replicationmembrane remodelingsupramolecular chemistry |
| spellingShingle | Rebeca Bocanegra María Ortiz-Rodríguez Ismael Plaza Garcia-Abadillo Carlos R-Pulido Borja Ibarra Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a Time Biophysica optical tweezers single-molecule manipulation DNA replication membrane remodeling supramolecular chemistry |
| title | Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a Time |
| title_full | Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a Time |
| title_fullStr | Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a Time |
| title_full_unstemmed | Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a Time |
| title_short | Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a Time |
| title_sort | optical tweezers to force information out of biological and synthetic systems one molecule at a time |
| topic | optical tweezers single-molecule manipulation DNA replication membrane remodeling supramolecular chemistry |
| url | https://www.mdpi.com/2673-4125/2/4/47 |
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