Growth under Different Trophic Regimes and Synchronization of the Red Microalga <i>Galdieria sulphuraria</i>
The extremophilic unicellular red microalga <i>Galdieria sulphuraria</i> (Cyanidiophyceae) is able to grow autotrophically, or mixo- and heterotrophically with 1% glycerol as a carbon source. The alga divides by multiple fission into more than two cells within one cell cycle. The optimal...
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MDPI AG
2021-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/7/939 |
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| author | Vít Náhlík Vilém Zachleder Mária Čížková Kateřina Bišová Anjali Singh Dana Mezricky Tomáš Řezanka Milada Vítová |
| author_facet | Vít Náhlík Vilém Zachleder Mária Čížková Kateřina Bišová Anjali Singh Dana Mezricky Tomáš Řezanka Milada Vítová |
| author_sort | Vít Náhlík |
| collection | DOAJ |
| description | The extremophilic unicellular red microalga <i>Galdieria sulphuraria</i> (Cyanidiophyceae) is able to grow autotrophically, or mixo- and heterotrophically with 1% glycerol as a carbon source. The alga divides by multiple fission into more than two cells within one cell cycle. The optimal conditions of light, temperature and pH (500 µmol photons m<sup>−2</sup> s<sup>−1</sup>, 40 °C, and pH 3; respectively) for the strain <i>Galdieria sulphuraria</i> (Galdieri) Merola 002 were determined as a basis for synchronization experiments. For synchronization, the specific light/dark cycle, 16/8 h was identified as the precondition for investigating the cell cycle. The alga was successfully synchronized and the cell cycle was evaluated. <i>G. sulphuraria</i> attained two commitment points with midpoints at 10 and 13 h of the cell cycle, leading to two nuclear divisions, followed subsequently by division into four daughter cells. The daughter cells stayed in the mother cell wall until the beginning of the next light phase, when they were released. Accumulation of glycogen throughout the cell cycle was also described. The findings presented here bring a new contribution to our general understanding of the cell cycle in cyanidialean red algae, and specifically of the biotechnologically important species <i>G. sulphuraria</i>. |
| first_indexed | 2024-03-09T04:55:42Z |
| format | Article |
| id | doaj.art-01166797d0354998b54421fc5ede4aee |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T04:55:42Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-01166797d0354998b54421fc5ede4aee2023-12-03T13:05:25ZengMDPI AGBiomolecules2218-273X2021-06-0111793910.3390/biom11070939Growth under Different Trophic Regimes and Synchronization of the Red Microalga <i>Galdieria sulphuraria</i>Vít Náhlík0Vilém Zachleder1Mária Čížková2Kateřina Bišová3Anjali Singh4Dana Mezricky5Tomáš Řezanka6Milada Vítová7Centre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Novohradská 237, 379 81 Třeboň, Czech RepublicCentre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Novohradská 237, 379 81 Třeboň, Czech RepublicCentre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Novohradská 237, 379 81 Třeboň, Czech RepublicCentre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Novohradská 237, 379 81 Třeboň, Czech RepublicCentre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Novohradská 237, 379 81 Třeboň, Czech RepublicDepartment of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences, Piaristengasse 1, 3500 Krems, AustriaInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech RepublicCentre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Novohradská 237, 379 81 Třeboň, Czech RepublicThe extremophilic unicellular red microalga <i>Galdieria sulphuraria</i> (Cyanidiophyceae) is able to grow autotrophically, or mixo- and heterotrophically with 1% glycerol as a carbon source. The alga divides by multiple fission into more than two cells within one cell cycle. The optimal conditions of light, temperature and pH (500 µmol photons m<sup>−2</sup> s<sup>−1</sup>, 40 °C, and pH 3; respectively) for the strain <i>Galdieria sulphuraria</i> (Galdieri) Merola 002 were determined as a basis for synchronization experiments. For synchronization, the specific light/dark cycle, 16/8 h was identified as the precondition for investigating the cell cycle. The alga was successfully synchronized and the cell cycle was evaluated. <i>G. sulphuraria</i> attained two commitment points with midpoints at 10 and 13 h of the cell cycle, leading to two nuclear divisions, followed subsequently by division into four daughter cells. The daughter cells stayed in the mother cell wall until the beginning of the next light phase, when they were released. Accumulation of glycogen throughout the cell cycle was also described. The findings presented here bring a new contribution to our general understanding of the cell cycle in cyanidialean red algae, and specifically of the biotechnologically important species <i>G. sulphuraria</i>.https://www.mdpi.com/2218-273X/11/7/939cell cyclered algae<i>Galdieria</i>growthcell divisionlight intensity |
| spellingShingle | Vít Náhlík Vilém Zachleder Mária Čížková Kateřina Bišová Anjali Singh Dana Mezricky Tomáš Řezanka Milada Vítová Growth under Different Trophic Regimes and Synchronization of the Red Microalga <i>Galdieria sulphuraria</i> Biomolecules cell cycle red algae <i>Galdieria</i> growth cell division light intensity |
| title | Growth under Different Trophic Regimes and Synchronization of the Red Microalga <i>Galdieria sulphuraria</i> |
| title_full | Growth under Different Trophic Regimes and Synchronization of the Red Microalga <i>Galdieria sulphuraria</i> |
| title_fullStr | Growth under Different Trophic Regimes and Synchronization of the Red Microalga <i>Galdieria sulphuraria</i> |
| title_full_unstemmed | Growth under Different Trophic Regimes and Synchronization of the Red Microalga <i>Galdieria sulphuraria</i> |
| title_short | Growth under Different Trophic Regimes and Synchronization of the Red Microalga <i>Galdieria sulphuraria</i> |
| title_sort | growth under different trophic regimes and synchronization of the red microalga i galdieria sulphuraria i |
| topic | cell cycle red algae <i>Galdieria</i> growth cell division light intensity |
| url | https://www.mdpi.com/2218-273X/11/7/939 |
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