Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.
The F region downstream of the mecI gene in the SCCmec element in hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) contains two bidirectionally overlapping open reading frames (ORFs), the fudoh ORF and the psm-mec ORF. The psm-mec ORF encodes a cytolysin, phenol-soluble modu...
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2011-02-01
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Series: | PLoS Pathogens |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21304931/pdf/?tool=EBI |
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author | Chikara Kaito Yuki Saito Gentaro Nagano Mariko Ikuo Yosuke Omae Yuichi Hanada Xiao Han Kyoko Kuwahara-Arai Tomomi Hishinuma Tadashi Baba Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu |
author_facet | Chikara Kaito Yuki Saito Gentaro Nagano Mariko Ikuo Yosuke Omae Yuichi Hanada Xiao Han Kyoko Kuwahara-Arai Tomomi Hishinuma Tadashi Baba Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu |
author_sort | Chikara Kaito |
collection | DOAJ |
description | The F region downstream of the mecI gene in the SCCmec element in hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) contains two bidirectionally overlapping open reading frames (ORFs), the fudoh ORF and the psm-mec ORF. The psm-mec ORF encodes a cytolysin, phenol-soluble modulin (PSM)-mec. Transformation of the F region into the Newman strain, which is a methicillin-sensitive S. aureus (MSSA) strain, or into the MW2 (USA400) and FRP3757 (USA300) strains, which are community-acquired MRSA (CA-MRSA) strains that lack the F region, attenuated their virulence in a mouse systemic infection model. Introducing the F region to these strains suppressed colony-spreading activity and PSMα production, and promoted biofilm formation. By producing mutations into the psm-mec ORF, we revealed that (i) both the transcription and translation products of the psm-mec ORF suppressed colony-spreading activity and promoted biofilm formation; and (ii) the transcription product of the psm-mec ORF, but not its translation product, decreased PSMα production. These findings suggest that both the psm-mec transcript, acting as a regulatory RNA, and the PSM-mec protein encoded by the gene on the mobile genetic element SCCmec regulate the virulence of Staphylococcus aureus. |
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issn | 1553-7366 1553-7374 |
language | English |
last_indexed | 2024-12-21T08:32:33Z |
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spelling | doaj.art-01181988587c4409935821e4ed22c88e2022-12-21T19:10:08ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742011-02-0172e100126710.1371/journal.ppat.1001267Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.Chikara KaitoYuki SaitoGentaro NaganoMariko IkuoYosuke OmaeYuichi HanadaXiao HanKyoko Kuwahara-AraiTomomi HishinumaTadashi BabaTeruyo ItoKeiichi HiramatsuKazuhisa SekimizuThe F region downstream of the mecI gene in the SCCmec element in hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) contains two bidirectionally overlapping open reading frames (ORFs), the fudoh ORF and the psm-mec ORF. The psm-mec ORF encodes a cytolysin, phenol-soluble modulin (PSM)-mec. Transformation of the F region into the Newman strain, which is a methicillin-sensitive S. aureus (MSSA) strain, or into the MW2 (USA400) and FRP3757 (USA300) strains, which are community-acquired MRSA (CA-MRSA) strains that lack the F region, attenuated their virulence in a mouse systemic infection model. Introducing the F region to these strains suppressed colony-spreading activity and PSMα production, and promoted biofilm formation. By producing mutations into the psm-mec ORF, we revealed that (i) both the transcription and translation products of the psm-mec ORF suppressed colony-spreading activity and promoted biofilm formation; and (ii) the transcription product of the psm-mec ORF, but not its translation product, decreased PSMα production. These findings suggest that both the psm-mec transcript, acting as a regulatory RNA, and the PSM-mec protein encoded by the gene on the mobile genetic element SCCmec regulate the virulence of Staphylococcus aureus.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21304931/pdf/?tool=EBI |
spellingShingle | Chikara Kaito Yuki Saito Gentaro Nagano Mariko Ikuo Yosuke Omae Yuichi Hanada Xiao Han Kyoko Kuwahara-Arai Tomomi Hishinuma Tadashi Baba Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. PLoS Pathogens |
title | Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. |
title_full | Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. |
title_fullStr | Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. |
title_full_unstemmed | Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. |
title_short | Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. |
title_sort | transcription and translation products of the cytolysin gene psm mec on the mobile genetic element sccmec regulate staphylococcus aureus virulence |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21304931/pdf/?tool=EBI |
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