Absence of CSF-1-Dependent Macrophages Does Not Improve Function of Transplanted Islets of Langerhans
A role of macrophage-mediated inflammatory events in early islet graft loss is increasingly acknowledged. Osteopetrotic mice (op/op) have a complete absence of CSF-1, and thus of most tissue macrophages. We have investigated whether the absence of CSF-1-dependent macrophages in the graft itself or a...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2001-10-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000001783986341 |
| _version_ | 1818060966068224000 |
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| author | Thierry Berney R. Damaris Molano Antonello Pileggi Pierre Cattan Caterina Vizzardelli Camillo Ricordi Luca Inverardi M.D. |
| author_facet | Thierry Berney R. Damaris Molano Antonello Pileggi Pierre Cattan Caterina Vizzardelli Camillo Ricordi Luca Inverardi M.D. |
| author_sort | Thierry Berney |
| collection | DOAJ |
| description | A role of macrophage-mediated inflammatory events in early islet graft loss is increasingly acknowledged. Osteopetrotic mice (op/op) have a complete absence of CSF-1, and thus of most tissue macrophages. We have investigated whether the absence of CSF-1-dependent macrophages in the graft itself or at the transplant site could decrease the delay to function of a syngeneic marginal islet mass. Islets transplanted into op/op or control recipients reversed diabetes in 59 days vs. 10 days (p = 0.28, NS). Islets isolated from op/op or control mice reversed diabetes in 11 days vs. 10 days. IL-1 and TNF-α release by cultured islets was markedly decreased for op/op islets compared with control islets (IL-1: 0 vs. 4.2 pg/ml, p = 0.07; TNF-α: 67 vs. 311 pg/ml, p = 0.002). In contrast, IL-6 release by op/op islets was significantly increased (11.1 vs. 4.3 ng/ml, p = 0.006). CSF-1-dependent tissue macrophages may not be critical in the inflammatory insult to islet transplants. Alternate patterns of intraislet release of deleterious proinflammatory cytokines may exist. |
| first_indexed | 2024-12-10T13:40:48Z |
| format | Article |
| id | doaj.art-011927d220af4dda9ea0b1d692c72c75 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-10T13:40:48Z |
| publishDate | 2001-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-011927d220af4dda9ea0b1d692c72c752022-12-22T01:46:41ZengSAGE PublishingCell Transplantation0963-68971555-38922001-10-011010.3727/000000001783986341Absence of CSF-1-Dependent Macrophages Does Not Improve Function of Transplanted Islets of LangerhansThierry Berney0R. Damaris Molano1Antonello Pileggi2Pierre Cattan3Caterina Vizzardelli4Camillo Ricordi5Luca Inverardi M.D.6Diabetes Research Institute, University of Miami Medical School, Miami, FL 33136Diabetes Research Institute, University of Miami Medical School, Miami, FL 33136Diabetes Research Institute, University of Miami Medical School, Miami, FL 33136Diabetes Research Institute, University of Miami Medical School, Miami, FL 33136Diabetes Research Institute, University of Miami Medical School, Miami, FL 33136Diabetes Research Institute, University of Miami Medical School, Miami, FL 33136Diabetes Research Institute, University of Miami Medical School, Miami, FL 33136A role of macrophage-mediated inflammatory events in early islet graft loss is increasingly acknowledged. Osteopetrotic mice (op/op) have a complete absence of CSF-1, and thus of most tissue macrophages. We have investigated whether the absence of CSF-1-dependent macrophages in the graft itself or at the transplant site could decrease the delay to function of a syngeneic marginal islet mass. Islets transplanted into op/op or control recipients reversed diabetes in 59 days vs. 10 days (p = 0.28, NS). Islets isolated from op/op or control mice reversed diabetes in 11 days vs. 10 days. IL-1 and TNF-α release by cultured islets was markedly decreased for op/op islets compared with control islets (IL-1: 0 vs. 4.2 pg/ml, p = 0.07; TNF-α: 67 vs. 311 pg/ml, p = 0.002). In contrast, IL-6 release by op/op islets was significantly increased (11.1 vs. 4.3 ng/ml, p = 0.006). CSF-1-dependent tissue macrophages may not be critical in the inflammatory insult to islet transplants. Alternate patterns of intraislet release of deleterious proinflammatory cytokines may exist.https://doi.org/10.3727/000000001783986341 |
| spellingShingle | Thierry Berney R. Damaris Molano Antonello Pileggi Pierre Cattan Caterina Vizzardelli Camillo Ricordi Luca Inverardi M.D. Absence of CSF-1-Dependent Macrophages Does Not Improve Function of Transplanted Islets of Langerhans Cell Transplantation |
| title | Absence of CSF-1-Dependent Macrophages Does Not Improve Function of Transplanted Islets of Langerhans |
| title_full | Absence of CSF-1-Dependent Macrophages Does Not Improve Function of Transplanted Islets of Langerhans |
| title_fullStr | Absence of CSF-1-Dependent Macrophages Does Not Improve Function of Transplanted Islets of Langerhans |
| title_full_unstemmed | Absence of CSF-1-Dependent Macrophages Does Not Improve Function of Transplanted Islets of Langerhans |
| title_short | Absence of CSF-1-Dependent Macrophages Does Not Improve Function of Transplanted Islets of Langerhans |
| title_sort | absence of csf 1 dependent macrophages does not improve function of transplanted islets of langerhans |
| url | https://doi.org/10.3727/000000001783986341 |
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