Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry
Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number a...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2000-09-01
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| Series: | Genetics and Molecular Biology |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300026 |
| _version_ | 1819156064171458560 |
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| author | Fernanda Januzzi Mendes-da-Glória Francisco de Assis Alves Mourão Filho Luis Eduardo Aranha Camargo Beatriz Madalena Januzzi Mendes |
| author_facet | Fernanda Januzzi Mendes-da-Glória Francisco de Assis Alves Mourão Filho Luis Eduardo Aranha Camargo Beatriz Madalena Januzzi Mendes |
| author_sort | Fernanda Januzzi Mendes-da-Glória |
| collection | DOAJ |
| description | Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.<br>Híbridos somáticos de laranja doce (Citrus sinensis L. Osbeck) e limão Cravo (C. limonia L. Osbeck) foram regenerados após a fusão (polietileno glicol) e cultura de protoplastos. Os híbridos somáticos foram confirmados pela análise da morfologia das folhas, determinação do número de cromossomos e marcadores moleculares (RAPD). Todas as plantas analisadas revelaram-se tetraplóides (2n = 4x = 36), possuíam folhas de morfologia intermediária e uma combinação do padrão de bandas de RAPD de ambos os parentais. Esta combinação pode se tornar útil como porta-enxerto para a Região Sudeste da indústria citrícola brasileira. Este híbrido somático potencialmente combinará as características de tolerância à seca e o vigor do limão Cravo com a tolerância ao declínio da laranja Caipira. |
| first_indexed | 2024-12-22T15:46:55Z |
| format | Article |
| id | doaj.art-011a999ffac341f1a77abdbf46e5082d |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-22T15:46:55Z |
| publishDate | 2000-09-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-011a999ffac341f1a77abdbf46e5082d2022-12-21T18:21:00ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852000-09-0123366166510.1590/S1415-47572000000300026Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industryFernanda Januzzi Mendes-da-GlóriaFrancisco de Assis Alves Mourão FilhoLuis Eduardo Aranha CamargoBeatriz Madalena Januzzi MendesProtoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.<br>Híbridos somáticos de laranja doce (Citrus sinensis L. Osbeck) e limão Cravo (C. limonia L. Osbeck) foram regenerados após a fusão (polietileno glicol) e cultura de protoplastos. Os híbridos somáticos foram confirmados pela análise da morfologia das folhas, determinação do número de cromossomos e marcadores moleculares (RAPD). Todas as plantas analisadas revelaram-se tetraplóides (2n = 4x = 36), possuíam folhas de morfologia intermediária e uma combinação do padrão de bandas de RAPD de ambos os parentais. Esta combinação pode se tornar útil como porta-enxerto para a Região Sudeste da indústria citrícola brasileira. Este híbrido somático potencialmente combinará as características de tolerância à seca e o vigor do limão Cravo com a tolerância ao declínio da laranja Caipira.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300026 |
| spellingShingle | Fernanda Januzzi Mendes-da-Glória Francisco de Assis Alves Mourão Filho Luis Eduardo Aranha Camargo Beatriz Madalena Januzzi Mendes Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry Genetics and Molecular Biology |
| title | Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry |
| title_full | Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry |
| title_fullStr | Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry |
| title_full_unstemmed | Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry |
| title_short | Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry |
| title_sort | caipira sweet orange rangpur lime a somatic hybrid with potential for use as rootstock in the brazilian citrus industry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300026 |
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