Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of Nanofibers
Atomic force microscopy (AFM) is a powerful tool that enables imaging and nanomechanical properties characterization of biological materials. Nanofibers are the structural units of many biological systems and their role in the development of advanced biomaterials is crucial. AFM methods have proven...
| المؤلفون الرئيسيون: | , , , |
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| التنسيق: | مقال |
| اللغة: | English |
| منشور في: |
MDPI AG
2023-10-01
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| سلاسل: | Fibers |
| الموضوعات: | |
| الوصول للمادة أونلاين: | https://www.mdpi.com/2079-6439/11/10/83 |
| _version_ | 1827720939832344576 |
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| author | Stylianos Vasileios Kontomaris Andreas Stylianou Georgios Chliveros Anna Malamou |
| author_facet | Stylianos Vasileios Kontomaris Andreas Stylianou Georgios Chliveros Anna Malamou |
| author_sort | Stylianos Vasileios Kontomaris |
| collection | DOAJ |
| description | Atomic force microscopy (AFM) is a powerful tool that enables imaging and nanomechanical properties characterization of biological materials. Nanofibers are the structural units of many biological systems and their role in the development of advanced biomaterials is crucial. AFM methods have proven to be effective towards the characterization of fibers with respect to biological and bioengineering applications at the nanoscale. However, both the topographical and mechanical properties’ nanocharacterizations of single fibers using AFM are challenging procedures. In particular, regarding imaging procedures, significant artifacts may arise from tip convolution effects. The geometrical characteristics of the AFM tip and the nanofibers, and the fact that they have similar magnitudes, may lead to significant errors regarding the topographical imaging. In addition, the determination of the mechanical properties of nanofibers is also challenging due to their small dimensions and heterogeneity (i.e., the elastic half-space assumption is not valid in most cases). This review elucidates the origins of errors in characterizing individual nanofibers, while also providing strategies to address limitations in experimental procedures and data processing. |
| first_indexed | 2024-03-10T21:15:16Z |
| format | Article |
| id | doaj.art-011cb2ed6ee44b90a9c1f47ac68d9800 |
| institution | Directory Open Access Journal |
| issn | 2079-6439 |
| language | English |
| last_indexed | 2024-03-10T21:15:16Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fibers |
| spelling | doaj.art-011cb2ed6ee44b90a9c1f47ac68d98002023-11-19T16:26:47ZengMDPI AGFibers2079-64392023-10-0111108310.3390/fib11100083Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of NanofibersStylianos Vasileios Kontomaris0Andreas Stylianou1Georgios Chliveros2Anna Malamou3Faculty of Engineering and Architecture, Metropolitan College, 15125 Athens, GreeceSchool of Sciences, European University Cyprus, Nicosia 2404, CyprusFaculty of Engineering and Architecture, Metropolitan College, 15125 Athens, GreeceIndependent Power Transmission Operator S.A. (IPTO), 10443 Athens, GreeceAtomic force microscopy (AFM) is a powerful tool that enables imaging and nanomechanical properties characterization of biological materials. Nanofibers are the structural units of many biological systems and their role in the development of advanced biomaterials is crucial. AFM methods have proven to be effective towards the characterization of fibers with respect to biological and bioengineering applications at the nanoscale. However, both the topographical and mechanical properties’ nanocharacterizations of single fibers using AFM are challenging procedures. In particular, regarding imaging procedures, significant artifacts may arise from tip convolution effects. The geometrical characteristics of the AFM tip and the nanofibers, and the fact that they have similar magnitudes, may lead to significant errors regarding the topographical imaging. In addition, the determination of the mechanical properties of nanofibers is also challenging due to their small dimensions and heterogeneity (i.e., the elastic half-space assumption is not valid in most cases). This review elucidates the origins of errors in characterizing individual nanofibers, while also providing strategies to address limitations in experimental procedures and data processing.https://www.mdpi.com/2079-6439/11/10/83AFM artifactstip convolution effectsmechanical propertiesbiological samplescontact mechanics modelsdata processing |
| spellingShingle | Stylianos Vasileios Kontomaris Andreas Stylianou Georgios Chliveros Anna Malamou Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of Nanofibers Fibers AFM artifacts tip convolution effects mechanical properties biological samples contact mechanics models data processing |
| title | Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of Nanofibers |
| title_full | Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of Nanofibers |
| title_fullStr | Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of Nanofibers |
| title_full_unstemmed | Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of Nanofibers |
| title_short | Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of Nanofibers |
| title_sort | overcoming challenges and limitations regarding the atomic force microscopy imaging and mechanical characterization of nanofibers |
| topic | AFM artifacts tip convolution effects mechanical properties biological samples contact mechanics models data processing |
| url | https://www.mdpi.com/2079-6439/11/10/83 |
| work_keys_str_mv | AT stylianosvasileioskontomaris overcomingchallengesandlimitationsregardingtheatomicforcemicroscopyimagingandmechanicalcharacterizationofnanofibers AT andreasstylianou overcomingchallengesandlimitationsregardingtheatomicforcemicroscopyimagingandmechanicalcharacterizationofnanofibers AT georgioschliveros overcomingchallengesandlimitationsregardingtheatomicforcemicroscopyimagingandmechanicalcharacterizationofnanofibers AT annamalamou overcomingchallengesandlimitationsregardingtheatomicforcemicroscopyimagingandmechanicalcharacterizationofnanofibers |