Liver-targeted liposomes for codelivery of curcumin and combretastatin A4 phosphate: preparation, characterization, and antitumor effects
Hong Jiang,1,* Zhi-Peng Li,1,* Gui-Xiang Tian,1,* Rui-Yan Pan,1 Chong-Mei Xu,2 Bo Zhang,2 Jing-liang Wu1 1School of Bioscience and Technology, Weifang Medical University, Weifang, Shandong, China; 2School of Pharmacy, Weifang Medical University, Weifang, Shandong, China *These authors contributed...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2019-03-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/liver-targeted-liposomes-for-codelivery-of-curcumin-and-combretastatin-peer-reviewed-article-IJN |
| _version_ | 1818852915628998656 |
|---|---|
| author | Jiang H Li ZP Tian GX Pan RY Xu CM Zhang B Wu JL |
| author_facet | Jiang H Li ZP Tian GX Pan RY Xu CM Zhang B Wu JL |
| author_sort | Jiang H |
| collection | DOAJ |
| description | Hong Jiang,1,* Zhi-Peng Li,1,* Gui-Xiang Tian,1,* Rui-Yan Pan,1 Chong-Mei Xu,2 Bo Zhang,2 Jing-liang Wu1 1School of Bioscience and Technology, Weifang Medical University, Weifang, Shandong, China; 2School of Pharmacy, Weifang Medical University, Weifang, Shandong, China *These authors contributed equally to this work Background: Recent efforts have been focused on combining two or more therapeutic approaches with different mechanisms to enhance antitumor therapy. Moreover, nanosize drug-delivery systems for codelivering two drugs with proapoptotic and antiangiogenic activities have exhibited great potential in efficient treatment of cancers. Methods: Glycyrrhetinic acid (GA)–modified liposomes (GA LPs) for liver-targeted codelivery of curcumin (Cur) and combretastatin A4 phosphate (CA4P) were prepared and characterized. In vitro cellular uptake, cytotoxicity, cell migration, in vivo biodistribution, antitumor activity, and histopathological studies were performed. Results: Compared with unmodified LPs (Cur-CA4P LPs), Cur-CA4P/GA LPs were taken up effectively by human hepatocellular carcinoma cells (BEL-7402) and showed higher cytotoxicity than free drugs. In vivo real-time near-infrared fluorescence–imaging results indicated that GA-targeted LPs increased accumulation in the tumor region. Moreover, Cur-CA4P/GA LPs showed stronger inhibition of tumor proliferation than Cur, Cur + CA4P, and Cur-CA4P LPs in vivo antitumor studies, which was also verified by H&E staining. Conclusion: GA-modified LPs can serve as a promising nanocarrier for liver-targeted co-delivery of antitumor drugs against hepatocellular carcinoma. Keywords: liver-targeted, LPs, curcumin, combretastatin A4 phosphate, combination therapy |
| first_indexed | 2024-12-19T07:28:30Z |
| format | Article |
| id | doaj.art-01217ca022574beb855971fa412a72ba |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-19T07:28:30Z |
| publishDate | 2019-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-01217ca022574beb855971fa412a72ba2022-12-21T20:30:47ZengDove Medical PressInternational Journal of Nanomedicine1178-20132019-03-01Volume 141789180444505Liver-targeted liposomes for codelivery of curcumin and combretastatin A4 phosphate: preparation, characterization, and antitumor effectsJiang HLi ZPTian GXPan RYXu CMZhang BWu JLHong Jiang,1,* Zhi-Peng Li,1,* Gui-Xiang Tian,1,* Rui-Yan Pan,1 Chong-Mei Xu,2 Bo Zhang,2 Jing-liang Wu1 1School of Bioscience and Technology, Weifang Medical University, Weifang, Shandong, China; 2School of Pharmacy, Weifang Medical University, Weifang, Shandong, China *These authors contributed equally to this work Background: Recent efforts have been focused on combining two or more therapeutic approaches with different mechanisms to enhance antitumor therapy. Moreover, nanosize drug-delivery systems for codelivering two drugs with proapoptotic and antiangiogenic activities have exhibited great potential in efficient treatment of cancers. Methods: Glycyrrhetinic acid (GA)–modified liposomes (GA LPs) for liver-targeted codelivery of curcumin (Cur) and combretastatin A4 phosphate (CA4P) were prepared and characterized. In vitro cellular uptake, cytotoxicity, cell migration, in vivo biodistribution, antitumor activity, and histopathological studies were performed. Results: Compared with unmodified LPs (Cur-CA4P LPs), Cur-CA4P/GA LPs were taken up effectively by human hepatocellular carcinoma cells (BEL-7402) and showed higher cytotoxicity than free drugs. In vivo real-time near-infrared fluorescence–imaging results indicated that GA-targeted LPs increased accumulation in the tumor region. Moreover, Cur-CA4P/GA LPs showed stronger inhibition of tumor proliferation than Cur, Cur + CA4P, and Cur-CA4P LPs in vivo antitumor studies, which was also verified by H&E staining. Conclusion: GA-modified LPs can serve as a promising nanocarrier for liver-targeted co-delivery of antitumor drugs against hepatocellular carcinoma. Keywords: liver-targeted, LPs, curcumin, combretastatin A4 phosphate, combination therapyhttps://www.dovepress.com/liver-targeted-liposomes-for-codelivery-of-curcumin-and-combretastatin-peer-reviewed-article-IJNLiver-targetedLPscurcumincombretastatin A4 phosphatecombination therapy |
| spellingShingle | Jiang H Li ZP Tian GX Pan RY Xu CM Zhang B Wu JL Liver-targeted liposomes for codelivery of curcumin and combretastatin A4 phosphate: preparation, characterization, and antitumor effects International Journal of Nanomedicine Liver-targeted LPs curcumin combretastatin A4 phosphate combination therapy |
| title | Liver-targeted liposomes for codelivery of curcumin and combretastatin A4 phosphate: preparation, characterization, and antitumor effects |
| title_full | Liver-targeted liposomes for codelivery of curcumin and combretastatin A4 phosphate: preparation, characterization, and antitumor effects |
| title_fullStr | Liver-targeted liposomes for codelivery of curcumin and combretastatin A4 phosphate: preparation, characterization, and antitumor effects |
| title_full_unstemmed | Liver-targeted liposomes for codelivery of curcumin and combretastatin A4 phosphate: preparation, characterization, and antitumor effects |
| title_short | Liver-targeted liposomes for codelivery of curcumin and combretastatin A4 phosphate: preparation, characterization, and antitumor effects |
| title_sort | liver targeted liposomes for codelivery of curcumin and combretastatin a4 phosphate preparation characterization and antitumor effects |
| topic | Liver-targeted LPs curcumin combretastatin A4 phosphate combination therapy |
| url | https://www.dovepress.com/liver-targeted-liposomes-for-codelivery-of-curcumin-and-combretastatin-peer-reviewed-article-IJN |
| work_keys_str_mv | AT jiangh livertargetedliposomesforcodeliveryofcurcuminandcombretastatina4phosphatepreparationcharacterizationandantitumoreffects AT lizp livertargetedliposomesforcodeliveryofcurcuminandcombretastatina4phosphatepreparationcharacterizationandantitumoreffects AT tiangx livertargetedliposomesforcodeliveryofcurcuminandcombretastatina4phosphatepreparationcharacterizationandantitumoreffects AT panry livertargetedliposomesforcodeliveryofcurcuminandcombretastatina4phosphatepreparationcharacterizationandantitumoreffects AT xucm livertargetedliposomesforcodeliveryofcurcuminandcombretastatina4phosphatepreparationcharacterizationandantitumoreffects AT zhangb livertargetedliposomesforcodeliveryofcurcuminandcombretastatina4phosphatepreparationcharacterizationandantitumoreffects AT wujl livertargetedliposomesforcodeliveryofcurcuminandcombretastatina4phosphatepreparationcharacterizationandantitumoreffects |