Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen'
Prunus avium cv. 'Fuchen' is a cultivated species with a high-profile economic benefit. Four media were used as a basal media equipped with four exogenous hormones to establish an intact regeneration system. Sodium thiosulfate (Na2S2O3) and active carbon (AC) improved the browning phenomen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2022-03-01
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| Series: | Horticultural Science |
| Subjects: | |
| Online Access: | https://hortsci.agriculturejournals.cz/artkey/hor-202201-0001_resolving-the-browning-during-the-establishment-of-the-in-vitro-propogation-of-prunus-avium-cv-fuchen.php |
| _version_ | 1797898953165897728 |
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| author | Yinkai Xi Biao Zeng Hengyu Huang Ye Wang Pinhui Yang |
| author_facet | Yinkai Xi Biao Zeng Hengyu Huang Ye Wang Pinhui Yang |
| author_sort | Yinkai Xi |
| collection | DOAJ |
| description | Prunus avium cv. 'Fuchen' is a cultivated species with a high-profile economic benefit. Four media were used as a basal media equipped with four exogenous hormones to establish an intact regeneration system. Sodium thiosulfate (Na2S2O3) and active carbon (AC) improved the browning phenomenon. The results indicated that 1.0 g/L of AC and 30 g/L of Na2S2O3 were the best combination to inhibit the browning on the optimal basal medium - olive medium (OM) (followed by woody plant medium). The OM containing 1.5 mg/L of zeatin, 2.0 mg/L of α-naphtalenaecetic acid (NAA) and 0.5 mg/L of kinetin was beneficial for the germination and growth of the axillary buds. Green bud points were visible at the base of the swollen base after 25 days with a high proliferation coefficient (more than 7.50). The most optimal medium for rooting was 1/2 OM combined with 1.0 mg/L of NAA and 0.5 mg/L of indole-3-butyric-acid with a 100% rooting rate. The survival rate was up to 100% after 60 days with acclimatisation. Generally, a high-effective regeneration system was established, which provides a reference for keeping the excellent traits of the cultivar. |
| first_indexed | 2024-04-10T08:21:15Z |
| format | Article |
| id | doaj.art-01244effc5474d01bb3f1375f79ff3e6 |
| institution | Directory Open Access Journal |
| issn | 0862-867X 1805-9333 |
| language | English |
| last_indexed | 2024-04-10T08:21:15Z |
| publishDate | 2022-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Horticultural Science |
| spelling | doaj.art-01244effc5474d01bb3f1375f79ff3e62023-02-23T03:35:36ZengCzech Academy of Agricultural SciencesHorticultural Science0862-867X1805-93332022-03-014911910.17221/51/2020-HORTSCIhor-202201-0001Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen'Yinkai Xi0Biao Zeng1Hengyu Huang2Ye Wang3Pinhui Yang4Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. ChinaMedicinal Plants Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, P.R. ChinaYunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. ChinaYunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. ChinaYunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. ChinaPrunus avium cv. 'Fuchen' is a cultivated species with a high-profile economic benefit. Four media were used as a basal media equipped with four exogenous hormones to establish an intact regeneration system. Sodium thiosulfate (Na2S2O3) and active carbon (AC) improved the browning phenomenon. The results indicated that 1.0 g/L of AC and 30 g/L of Na2S2O3 were the best combination to inhibit the browning on the optimal basal medium - olive medium (OM) (followed by woody plant medium). The OM containing 1.5 mg/L of zeatin, 2.0 mg/L of α-naphtalenaecetic acid (NAA) and 0.5 mg/L of kinetin was beneficial for the germination and growth of the axillary buds. Green bud points were visible at the base of the swollen base after 25 days with a high proliferation coefficient (more than 7.50). The most optimal medium for rooting was 1/2 OM combined with 1.0 mg/L of NAA and 0.5 mg/L of indole-3-butyric-acid with a 100% rooting rate. The survival rate was up to 100% after 60 days with acclimatisation. Generally, a high-effective regeneration system was established, which provides a reference for keeping the excellent traits of the cultivar.https://hortsci.agriculturejournals.cz/artkey/hor-202201-0001_resolving-the-browning-during-the-establishment-of-the-in-vitro-propogation-of-prunus-avium-cv-fuchen.phpsweet cherry 'fuchen'bud proliferationbasal mediumrapid propagationbrowning inhibition |
| spellingShingle | Yinkai Xi Biao Zeng Hengyu Huang Ye Wang Pinhui Yang Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen' Horticultural Science sweet cherry 'fuchen' bud proliferation basal medium rapid propagation browning inhibition |
| title | Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen' |
| title_full | Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen' |
| title_fullStr | Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen' |
| title_full_unstemmed | Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen' |
| title_short | Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen' |
| title_sort | resolving the browning during the establishment of the in vitro propogation of prunus avium cv fuchen |
| topic | sweet cherry 'fuchen' bud proliferation basal medium rapid propagation browning inhibition |
| url | https://hortsci.agriculturejournals.cz/artkey/hor-202201-0001_resolving-the-browning-during-the-establishment-of-the-in-vitro-propogation-of-prunus-avium-cv-fuchen.php |
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