Multi-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functions
Abstract Despite the wealth of publicly available single-cell datasets, our understanding of distinct resident immune cells and their unique features in diverse human organs remains limited. To address this, we compiled a meta-analysis dataset of 114,275 CD45+ immune cells sourced from 14 organs in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-024-03152-z |
| _version_ | 1797233797620563968 |
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| author | Maria Tsagiopoulou Sonal Rashmi Sergio Aguilar-Fernandez Juan Nieto Ivo G. Gut |
| author_facet | Maria Tsagiopoulou Sonal Rashmi Sergio Aguilar-Fernandez Juan Nieto Ivo G. Gut |
| author_sort | Maria Tsagiopoulou |
| collection | DOAJ |
| description | Abstract Despite the wealth of publicly available single-cell datasets, our understanding of distinct resident immune cells and their unique features in diverse human organs remains limited. To address this, we compiled a meta-analysis dataset of 114,275 CD45+ immune cells sourced from 14 organs in healthy donors. While the transcriptome of immune cells remains relatively consistent across organs, our analysis has unveiled organ-specific gene expression differences (GTPX3 in kidney, DNTT and ACVR2B in thymus). These alterations are linked to different transcriptional factor activities and pathways including metabolism. TNF-α signaling through the NFkB pathway was found in several organs and immune compartments. The presence of distinct expression profiles for NFkB family genes and their target genes, including cytokines, underscores their pivotal role in cell positioning. Taken together, immune cells serve a dual role: safeguarding the organs and dynamically adjusting to the intricacies of the host organ environment, thereby actively contributing to its functionality and overall homeostasis. |
| first_indexed | 2024-04-24T16:21:53Z |
| format | Article |
| id | doaj.art-0124732cb7f644b6a87552e2e7fd3055 |
| institution | Directory Open Access Journal |
| issn | 2052-4463 |
| language | English |
| last_indexed | 2024-04-24T16:21:53Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj.art-0124732cb7f644b6a87552e2e7fd30552024-03-31T11:10:24ZengNature PortfolioScientific Data2052-44632024-03-0111111310.1038/s41597-024-03152-zMulti-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functionsMaria Tsagiopoulou0Sonal Rashmi1Sergio Aguilar-Fernandez2Juan Nieto3Ivo G. Gut4Centro Nacional de Analisis Genomico (CNAG)Centro Nacional de Analisis Genomico (CNAG)Centro Nacional de Analisis Genomico (CNAG)Centro Nacional de Analisis Genomico (CNAG)Centro Nacional de Analisis Genomico (CNAG)Abstract Despite the wealth of publicly available single-cell datasets, our understanding of distinct resident immune cells and their unique features in diverse human organs remains limited. To address this, we compiled a meta-analysis dataset of 114,275 CD45+ immune cells sourced from 14 organs in healthy donors. While the transcriptome of immune cells remains relatively consistent across organs, our analysis has unveiled organ-specific gene expression differences (GTPX3 in kidney, DNTT and ACVR2B in thymus). These alterations are linked to different transcriptional factor activities and pathways including metabolism. TNF-α signaling through the NFkB pathway was found in several organs and immune compartments. The presence of distinct expression profiles for NFkB family genes and their target genes, including cytokines, underscores their pivotal role in cell positioning. Taken together, immune cells serve a dual role: safeguarding the organs and dynamically adjusting to the intricacies of the host organ environment, thereby actively contributing to its functionality and overall homeostasis.https://doi.org/10.1038/s41597-024-03152-z |
| spellingShingle | Maria Tsagiopoulou Sonal Rashmi Sergio Aguilar-Fernandez Juan Nieto Ivo G. Gut Multi-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functions Scientific Data |
| title | Multi-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functions |
| title_full | Multi-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functions |
| title_fullStr | Multi-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functions |
| title_full_unstemmed | Multi-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functions |
| title_short | Multi-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functions |
| title_sort | multi organ single cell transcriptomics of immune cells uncovered organ specific gene expression and functions |
| url | https://doi.org/10.1038/s41597-024-03152-z |
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