Protective effects of apocynin and melatonin on ovarian ischemia/reperfusion injury in rats
Objective: This study aims to determine the protective effects of apocynin, a NADPH oxidase inhibitor, and melatonin, an endogenous anti-oxidant, in an animal model of ovarian ischemia/reperfusion (I/R) injury. Materials/Methods: Thirty-five female rats were randomly divided into five groups, namely...
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| Format: | Article |
| Language: | English |
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IMR Press
2021-02-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | https://www.imrpress.com/journal/CEOG/48/1/10.31083/j.ceog.2021.01.5488 |
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| author | Çiğdem Kunt İşgüder Murat Uysal Özlem Koşar Can Mine Kanat Pektaş Akgül Arici Velid Unsal |
| author_facet | Çiğdem Kunt İşgüder Murat Uysal Özlem Koşar Can Mine Kanat Pektaş Akgül Arici Velid Unsal |
| author_sort | Çiğdem Kunt İşgüder |
| collection | DOAJ |
| description | Objective: This study aims to determine the protective effects of apocynin, a NADPH oxidase inhibitor, and melatonin, an endogenous anti-oxidant, in an animal model of ovarian ischemia/reperfusion (I/R) injury. Materials/Methods: Thirty-five female rats were randomly divided into five groups, namely group I (sham), group II (I/R), group III (I/R + 10 mg/kg apocynin), group IV (I/R + 20 mg/kg apocynin), and group V (I/R + 10 mg/kg melatonin). Ovarian tissue and serum superoxide dismutase (SOD) and catalase (CAT) activities and malondialdehyde (MDA) and protein carbonyl (PC) levels were measured. Ovarian histopathology was examined and Bax, caspase 3, and iNOS immunoreactivities were evaluated. Results: Preoperative apocynin and melatonin significantly increased SOD and CAT activities (P < 0.05, P < 0.05, P < 0.01, P < 0.01, P < 0.01, P < 0.05, P < 0.05, and P < 0.01, respectively, for both apocynin and melatonin). In addition, preoperative apocynin and melatonin significantly decreased the ovarian I/R injury score (P < 0.01 for both). Bax, caspase 3, and iNOS immunoreactivities were significantly lower in the I/R + 10 mg/kg apocynin and I/R + 10 mg/kg melatonin groups than in the I/R group (P < 0.01, P < 0.01, P < 0.01, P < 0.01, P < 0.05, and P < 0.01, respectively). Conclusions: Apocynin and melatonin are powerful anti-oxidant agents with considerable bioavailability and safety. Preoperative apocynin and melatonin administration might protect ovarian tissue from I/R injury after surgical adnexal detorsion. |
| first_indexed | 2024-12-12T05:21:00Z |
| format | Article |
| id | doaj.art-01247edd0d194dadb63c5d33c91e0723 |
| institution | Directory Open Access Journal |
| issn | 0390-6663 |
| language | English |
| last_indexed | 2024-12-12T05:21:00Z |
| publishDate | 2021-02-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj.art-01247edd0d194dadb63c5d33c91e07232022-12-22T00:36:37ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632021-02-014819810410.31083/j.ceog.2021.01.5488S0390-6663(21)00051-8Protective effects of apocynin and melatonin on ovarian ischemia/reperfusion injury in ratsÇiğdem Kunt İşgüder0Murat Uysal1Özlem Koşar Can2Mine Kanat Pektaş3Akgül Arici4Velid Unsal5Department of Obstetrics and Gynecology, Faculty of Medicine Istanbul, Istanbul University, 34452 Istanbul, TurkeyDepartment of Anatomy, Faculty of Medicine, Gaziosmanpasa University, 60250 Tokat, TurkeyDepartment of Obstetrics and Gynecology, Faculty of Medicine, Pamukkale University, 20160 Denizli, TurkeyDepartment of Obstetrics and Gynecology, Faculty of Medicine, Afyonkarahisar University of Health Sciences, 03200 Afyonkarahisar, TurkeyDepartment of Pathology, Faculty of Medicine, Gaziosmanpasa University, 60250 Tokat, TurkeyFaculty of Health Sciences and Central Research Laboratory, Mardin Artuklu University, 47200 Mardin, TurkeyObjective: This study aims to determine the protective effects of apocynin, a NADPH oxidase inhibitor, and melatonin, an endogenous anti-oxidant, in an animal model of ovarian ischemia/reperfusion (I/R) injury. Materials/Methods: Thirty-five female rats were randomly divided into five groups, namely group I (sham), group II (I/R), group III (I/R + 10 mg/kg apocynin), group IV (I/R + 20 mg/kg apocynin), and group V (I/R + 10 mg/kg melatonin). Ovarian tissue and serum superoxide dismutase (SOD) and catalase (CAT) activities and malondialdehyde (MDA) and protein carbonyl (PC) levels were measured. Ovarian histopathology was examined and Bax, caspase 3, and iNOS immunoreactivities were evaluated. Results: Preoperative apocynin and melatonin significantly increased SOD and CAT activities (P < 0.05, P < 0.05, P < 0.01, P < 0.01, P < 0.01, P < 0.05, P < 0.05, and P < 0.01, respectively, for both apocynin and melatonin). In addition, preoperative apocynin and melatonin significantly decreased the ovarian I/R injury score (P < 0.01 for both). Bax, caspase 3, and iNOS immunoreactivities were significantly lower in the I/R + 10 mg/kg apocynin and I/R + 10 mg/kg melatonin groups than in the I/R group (P < 0.01, P < 0.01, P < 0.01, P < 0.01, P < 0.05, and P < 0.01, respectively). Conclusions: Apocynin and melatonin are powerful anti-oxidant agents with considerable bioavailability and safety. Preoperative apocynin and melatonin administration might protect ovarian tissue from I/R injury after surgical adnexal detorsion.https://www.imrpress.com/journal/CEOG/48/1/10.31083/j.ceog.2021.01.5488apocyninischemiamelatoninovaryreperfusion injury |
| spellingShingle | Çiğdem Kunt İşgüder Murat Uysal Özlem Koşar Can Mine Kanat Pektaş Akgül Arici Velid Unsal Protective effects of apocynin and melatonin on ovarian ischemia/reperfusion injury in rats Clinical and Experimental Obstetrics & Gynecology apocynin ischemia melatonin ovary reperfusion injury |
| title | Protective effects of apocynin and melatonin on ovarian ischemia/reperfusion injury in rats |
| title_full | Protective effects of apocynin and melatonin on ovarian ischemia/reperfusion injury in rats |
| title_fullStr | Protective effects of apocynin and melatonin on ovarian ischemia/reperfusion injury in rats |
| title_full_unstemmed | Protective effects of apocynin and melatonin on ovarian ischemia/reperfusion injury in rats |
| title_short | Protective effects of apocynin and melatonin on ovarian ischemia/reperfusion injury in rats |
| title_sort | protective effects of apocynin and melatonin on ovarian ischemia reperfusion injury in rats |
| topic | apocynin ischemia melatonin ovary reperfusion injury |
| url | https://www.imrpress.com/journal/CEOG/48/1/10.31083/j.ceog.2021.01.5488 |
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