Protective Roles of N-acetyl Cysteine and/or Taurine against Sumatriptan-Induced Hepatotoxicity
Purpose: Triptans are the drug category mostly prescribed for abortive treatment of migraine. Most recent cases of liver toxicity induced by triptans have been described, but the mechanisms of liver toxicity of these medications have not been clear. Methods: In the present study, we obtained LC50 u...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2016-12-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-627.pdf |
| _version_ | 1818553801825583104 |
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| author | Javad Khalili Fard Hossein Hamzeiy Mohammadreza Sattari Mohammad Ali Eghbal |
| author_facet | Javad Khalili Fard Hossein Hamzeiy Mohammadreza Sattari Mohammad Ali Eghbal |
| author_sort | Javad Khalili Fard |
| collection | DOAJ |
| description | Purpose: Triptans are the drug category mostly prescribed for abortive treatment of migraine. Most recent cases of liver toxicity induced by triptans have been described, but the mechanisms of liver toxicity of these medications have not been clear.
Methods: In the present study, we obtained LC50 using dose-response curve and investigated cell viability, free radical generation, lipid peroxide production, mitochondrial injury, lysosomal membrane damage and the cellular glutathione level as toxicity markers as well as the beneficial effects of taurine and/or N-acetyl cysteine in the sumatriptan-treated rat parenchymal hepatocytes using accelerated method of cytotoxicity mechanism screening.
Results: It was revealed that liver toxicity induced by sumatriptan in in freshly isolated parenchymal hepatocytes is dose-dependent. Sumatriptan caused significant free radical generation followed by lipid peroxide formation, mitochondrial injury as well as lysosomal damage. Moreover, sumatriptan reduced cellular glutathione content. Taurine and N-acetyl cysteine were able to protect hepatocytes against sumatriptan-induced harmful effects.
Conclusion: It is concluded that sumatriptan causes oxidative stress in hepatocytes and the decreased hepatocytes glutathione has a key role in the sumatriptan-induced harmful effects. Also, N-acetyl cysteine and/or taurine could be used as treatments in sumatriptan-induced side effects. |
| first_indexed | 2024-12-12T09:30:34Z |
| format | Article |
| id | doaj.art-012485380c404d9c8ceae5a62520e57a |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-12T09:30:34Z |
| publishDate | 2016-12-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-012485380c404d9c8ceae5a62520e57a2022-12-22T00:28:51ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082016-12-016462763710.15171/apb.2016.077APB_201_20160915040418Protective Roles of N-acetyl Cysteine and/or Taurine against Sumatriptan-Induced HepatotoxicityJavad Khalili Fard0Hossein Hamzeiy1Mohammadreza Sattari2Mohammad Ali Eghbal3Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Purpose: Triptans are the drug category mostly prescribed for abortive treatment of migraine. Most recent cases of liver toxicity induced by triptans have been described, but the mechanisms of liver toxicity of these medications have not been clear. Methods: In the present study, we obtained LC50 using dose-response curve and investigated cell viability, free radical generation, lipid peroxide production, mitochondrial injury, lysosomal membrane damage and the cellular glutathione level as toxicity markers as well as the beneficial effects of taurine and/or N-acetyl cysteine in the sumatriptan-treated rat parenchymal hepatocytes using accelerated method of cytotoxicity mechanism screening. Results: It was revealed that liver toxicity induced by sumatriptan in in freshly isolated parenchymal hepatocytes is dose-dependent. Sumatriptan caused significant free radical generation followed by lipid peroxide formation, mitochondrial injury as well as lysosomal damage. Moreover, sumatriptan reduced cellular glutathione content. Taurine and N-acetyl cysteine were able to protect hepatocytes against sumatriptan-induced harmful effects. Conclusion: It is concluded that sumatriptan causes oxidative stress in hepatocytes and the decreased hepatocytes glutathione has a key role in the sumatriptan-induced harmful effects. Also, N-acetyl cysteine and/or taurine could be used as treatments in sumatriptan-induced side effects.http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-627.pdfOxidative StressMitochondriaN-acetyl cysteineSumatriptanTaurineToxicity |
| spellingShingle | Javad Khalili Fard Hossein Hamzeiy Mohammadreza Sattari Mohammad Ali Eghbal Protective Roles of N-acetyl Cysteine and/or Taurine against Sumatriptan-Induced Hepatotoxicity Advanced Pharmaceutical Bulletin Oxidative Stress Mitochondria N-acetyl cysteine Sumatriptan Taurine Toxicity |
| title | Protective Roles of N-acetyl Cysteine and/or Taurine against Sumatriptan-Induced Hepatotoxicity |
| title_full | Protective Roles of N-acetyl Cysteine and/or Taurine against Sumatriptan-Induced Hepatotoxicity |
| title_fullStr | Protective Roles of N-acetyl Cysteine and/or Taurine against Sumatriptan-Induced Hepatotoxicity |
| title_full_unstemmed | Protective Roles of N-acetyl Cysteine and/or Taurine against Sumatriptan-Induced Hepatotoxicity |
| title_short | Protective Roles of N-acetyl Cysteine and/or Taurine against Sumatriptan-Induced Hepatotoxicity |
| title_sort | protective roles of n acetyl cysteine and or taurine against sumatriptan induced hepatotoxicity |
| topic | Oxidative Stress Mitochondria N-acetyl cysteine Sumatriptan Taurine Toxicity |
| url | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-627.pdf |
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