Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology
The aim of this study was to investigate protein regulation at different time points during the in vitro maturation of yak oocytes. Yak oocytes at GV, MI, and MII stages were collected during in vitro maturation, and differential proteomics sequencing was performed using iTRAQ technology. GO functio...
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| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/13/2085 |
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| author | Xin Ma Meng Wang Jinglei Wang Qian Zhang Sisi Pu Rui Wang Sijiu Yu Libin Wang Yangyang Pan |
| author_facet | Xin Ma Meng Wang Jinglei Wang Qian Zhang Sisi Pu Rui Wang Sijiu Yu Libin Wang Yangyang Pan |
| author_sort | Xin Ma |
| collection | DOAJ |
| description | The aim of this study was to investigate protein regulation at different time points during the in vitro maturation of yak oocytes. Yak oocytes at GV, MI, and MII stages were collected during in vitro maturation, and differential proteomics sequencing was performed using iTRAQ technology. GO functional classification indicated that the differential proteins were closely associated with biological processes such as “metabolic processes”, and molecular events such as “binding” molecular-function-related categories were active. KOG analysis showed that energy-metabolism-related activities were vigorous during oocyte development from the GV phase to MI phase, and genetic material preparation activities were more active when oocytes developed from the MI stage to MII stage. KEGG pathway analysis showed that the PPAR metabolic pathway, Hippo signaling pathway, and ECM–receptor interaction and metabolic pathway were enriched from the GV to the MI stages. The PI3K-Akt, TGF-β, and phagosome pathways were enriched from the MI stage to the MII stage. These results indicate that transient dynamic changes occurred in the proteome during the maturation of yak oocytes, and the physiological functions mediated by these were also different. The accurate identification of the differential proteins in the three stages of GV, MI, and MII was helpful in further analyzing the molecular regulatory mechanism of yak oocyte maturation. |
| first_indexed | 2024-03-11T01:48:39Z |
| format | Article |
| id | doaj.art-012ce568a1bf467ea7844aa0c553dc49 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:48:39Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-012ce568a1bf467ea7844aa0c553dc492023-11-18T16:02:43ZengMDPI AGAnimals2076-26152023-06-011313208510.3390/ani13132085Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ TechnologyXin Ma0Meng Wang1Jinglei Wang2Qian Zhang3Sisi Pu4Rui Wang5Sijiu Yu6Libin Wang7Yangyang Pan8College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaThe aim of this study was to investigate protein regulation at different time points during the in vitro maturation of yak oocytes. Yak oocytes at GV, MI, and MII stages were collected during in vitro maturation, and differential proteomics sequencing was performed using iTRAQ technology. GO functional classification indicated that the differential proteins were closely associated with biological processes such as “metabolic processes”, and molecular events such as “binding” molecular-function-related categories were active. KOG analysis showed that energy-metabolism-related activities were vigorous during oocyte development from the GV phase to MI phase, and genetic material preparation activities were more active when oocytes developed from the MI stage to MII stage. KEGG pathway analysis showed that the PPAR metabolic pathway, Hippo signaling pathway, and ECM–receptor interaction and metabolic pathway were enriched from the GV to the MI stages. The PI3K-Akt, TGF-β, and phagosome pathways were enriched from the MI stage to the MII stage. These results indicate that transient dynamic changes occurred in the proteome during the maturation of yak oocytes, and the physiological functions mediated by these were also different. The accurate identification of the differential proteins in the three stages of GV, MI, and MII was helpful in further analyzing the molecular regulatory mechanism of yak oocyte maturation.https://www.mdpi.com/2076-2615/13/13/2085oocytesiTRAQyakdifferential proteomics |
| spellingShingle | Xin Ma Meng Wang Jinglei Wang Qian Zhang Sisi Pu Rui Wang Sijiu Yu Libin Wang Yangyang Pan Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology Animals oocytes iTRAQ yak differential proteomics |
| title | Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology |
| title_full | Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology |
| title_fullStr | Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology |
| title_full_unstemmed | Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology |
| title_short | Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology |
| title_sort | dynamic changes in proteome during yak oocyte maturation analyzed using itraq technology |
| topic | oocytes iTRAQ yak differential proteomics |
| url | https://www.mdpi.com/2076-2615/13/13/2085 |
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