Prevalence of Enterobacteriaceae in Camel, Cattle, and Sheep Carcasses at Slaughterhouses and Butcher Shops
Enterobacteriaceae can contaminate meat during various processing stages, including slaughter, evisceration, and b utchering, potentially causing foodborne illnesses. The purpose of this study was to investigate the prevalence of Enterobacteriaceae in carcass samples obtained from slaughterhouses an...
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MDPI AG
2023-10-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/20/11495 |
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| author | Fahad Al-Asmari Siddig H. Hamad Salah A. Al Hashedi |
| author_facet | Fahad Al-Asmari Siddig H. Hamad Salah A. Al Hashedi |
| author_sort | Fahad Al-Asmari |
| collection | DOAJ |
| description | Enterobacteriaceae can contaminate meat during various processing stages, including slaughter, evisceration, and b utchering, potentially causing foodborne illnesses. The purpose of this study was to investigate the prevalence of Enterobacteriaceae in carcass samples obtained from slaughterhouses and meat cuts collected from butcher shops. A total of 120 samples of camel, cattle, and sheep meat were analyzed for microbial contamination and isolates were identified using the PCR test. Total viable count ranged from 4.91 to 5.37 Log<sub>10</sub> CFU/g in slaughterhouses and butcher shops. <i>E. coli</i> dominated, with 84 out of the 120 samples (70%) contaminated, where contamination was highest in camel meat and lowest in sheep meat with 100% and 30% of contaminated samples, respectively. Salmonella was confirmed in 40% of camel, 47.5% of cattle, and 32.5% of sheep samples. In addition, twenty-five Enterobacteriaceae strains belonging to 19 different genera were detected in the meat samples. The highest occurrence was in the sheep samples with 15 different genera followed by the camels and the cattle samples with 14 different genera each. The presence of Enterobacteriaceae in camel, cattle, and sheep carcasses raises significant concerns regarding food safety. Adherence to good hygiene practices throughout animal slaughtering is crucial to minimize the risk of infection and transmission and ensure food safety. |
| first_indexed | 2024-03-10T21:27:58Z |
| format | Article |
| id | doaj.art-012fbed617214bd6b70f3fc81361a461 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T21:27:58Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-012fbed617214bd6b70f3fc81361a4612023-11-19T15:33:00ZengMDPI AGApplied Sciences2076-34172023-10-0113201149510.3390/app132011495Prevalence of Enterobacteriaceae in Camel, Cattle, and Sheep Carcasses at Slaughterhouses and Butcher ShopsFahad Al-Asmari0Siddig H. Hamad1Salah A. Al Hashedi2Department of Food Science and Nutrition, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi ArabiaDepartment of Food Science and Nutrition, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi ArabiaCentra labs, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi ArabiaEnterobacteriaceae can contaminate meat during various processing stages, including slaughter, evisceration, and b utchering, potentially causing foodborne illnesses. The purpose of this study was to investigate the prevalence of Enterobacteriaceae in carcass samples obtained from slaughterhouses and meat cuts collected from butcher shops. A total of 120 samples of camel, cattle, and sheep meat were analyzed for microbial contamination and isolates were identified using the PCR test. Total viable count ranged from 4.91 to 5.37 Log<sub>10</sub> CFU/g in slaughterhouses and butcher shops. <i>E. coli</i> dominated, with 84 out of the 120 samples (70%) contaminated, where contamination was highest in camel meat and lowest in sheep meat with 100% and 30% of contaminated samples, respectively. Salmonella was confirmed in 40% of camel, 47.5% of cattle, and 32.5% of sheep samples. In addition, twenty-five Enterobacteriaceae strains belonging to 19 different genera were detected in the meat samples. The highest occurrence was in the sheep samples with 15 different genera followed by the camels and the cattle samples with 14 different genera each. The presence of Enterobacteriaceae in camel, cattle, and sheep carcasses raises significant concerns regarding food safety. Adherence to good hygiene practices throughout animal slaughtering is crucial to minimize the risk of infection and transmission and ensure food safety.https://www.mdpi.com/2076-3417/13/20/11495Enterobacteriaceaecamelscattlesheepslaughterhousesbutcher shops |
| spellingShingle | Fahad Al-Asmari Siddig H. Hamad Salah A. Al Hashedi Prevalence of Enterobacteriaceae in Camel, Cattle, and Sheep Carcasses at Slaughterhouses and Butcher Shops Applied Sciences Enterobacteriaceae camels cattle sheep slaughterhouses butcher shops |
| title | Prevalence of Enterobacteriaceae in Camel, Cattle, and Sheep Carcasses at Slaughterhouses and Butcher Shops |
| title_full | Prevalence of Enterobacteriaceae in Camel, Cattle, and Sheep Carcasses at Slaughterhouses and Butcher Shops |
| title_fullStr | Prevalence of Enterobacteriaceae in Camel, Cattle, and Sheep Carcasses at Slaughterhouses and Butcher Shops |
| title_full_unstemmed | Prevalence of Enterobacteriaceae in Camel, Cattle, and Sheep Carcasses at Slaughterhouses and Butcher Shops |
| title_short | Prevalence of Enterobacteriaceae in Camel, Cattle, and Sheep Carcasses at Slaughterhouses and Butcher Shops |
| title_sort | prevalence of enterobacteriaceae in camel cattle and sheep carcasses at slaughterhouses and butcher shops |
| topic | Enterobacteriaceae camels cattle sheep slaughterhouses butcher shops |
| url | https://www.mdpi.com/2076-3417/13/20/11495 |
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