Functionality-packed additively manufactured porous titanium implants
The holy grail of orthopedic implant design is to ward off both aseptic and septic loosening for long enough that the implant outlives the patient. Questing this holy grail is feasible only if orthopedic biomaterials possess a long list of functionalities that enable them to discharge the onerous ta...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-06-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259000642030020X |
| _version_ | 1818308845113442304 |
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| author | I.A.J. van Hengel F.S.A. Gelderman S. Athanasiadis M. Minneboo H. Weinans A.C. Fluit B.C.J. van der Eerden L.E. Fratila-Apachitei I. Apachitei A.A. Zadpoor |
| author_facet | I.A.J. van Hengel F.S.A. Gelderman S. Athanasiadis M. Minneboo H. Weinans A.C. Fluit B.C.J. van der Eerden L.E. Fratila-Apachitei I. Apachitei A.A. Zadpoor |
| author_sort | I.A.J. van Hengel |
| collection | DOAJ |
| description | The holy grail of orthopedic implant design is to ward off both aseptic and septic loosening for long enough that the implant outlives the patient. Questing this holy grail is feasible only if orthopedic biomaterials possess a long list of functionalities that enable them to discharge the onerous task of permanently replacing the native bone tissue. Here, we present a rationally designed and additive manufacturing (AM) topologically ordered porous metallic biomaterial that is made from Ti-6Al-4V using selective laser melting and packs most (if not all) of the required functionalities into a single implant. In addition to presenting a fully interconnected porous structure and form-freedom that enables realization of patient-specific implants, the biomaterials developed here were biofunctionalized using plasma electrolytic oxidation to locally release both osteogenic (i.e. strontium) and antibacterial (i.e. silver ions) agents. The same single-step biofunctionalization process also incorporated hydroxyapatite into the surface of the implants. Our measurements verified the continued release of both types of active agents up to 28 days. Assessment of the antibacterial activity in vitro and in an ex vivo murine model demonstrated extraordinarily high levels of bactericidal effects against a highly virulent and multidrug-resistant Staphylococcus aureus strain (i.e. USA300) with total eradication of both planktonic and adherent bacteria. This strong antibacterial behavior was combined with a significantly enhanced osteogenic behavior, as evidenced by significantly higher levels of alkaline phosphatase (ALP) activity compared with non-biofunctionalized implants. Finally, we discovered synergistic antibacterial behavior between strontium and silver ions, meaning that 4–32 folds lower concentrations of silver ions were required to achieve growth inhibition and total killing of bacteria. The functionality-packed biomaterial presented here demonstrates a unique combination of functionalities that make it an advanced prototype of future orthopedic biomaterials where implants will outlive patients. |
| first_indexed | 2024-12-13T07:20:44Z |
| format | Article |
| id | doaj.art-0131cc0093e84019b76e318003b32054 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-12-13T07:20:44Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-0131cc0093e84019b76e318003b320542022-12-21T23:55:25ZengElsevierMaterials Today Bio2590-00642020-06-017100060Functionality-packed additively manufactured porous titanium implantsI.A.J. van Hengel0F.S.A. Gelderman1S. Athanasiadis2M. Minneboo3H. Weinans4A.C. Fluit5B.C.J. van der Eerden6L.E. Fratila-Apachitei7I. Apachitei8A.A. Zadpoor9Additive Manufacturing Laboratory, Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, the Netherlands; Corresponding author.Additive Manufacturing Laboratory, Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, the NetherlandsAdditive Manufacturing Laboratory, Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, the NetherlandsAdditive Manufacturing Laboratory, Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, the NetherlandsAdditive Manufacturing Laboratory, Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, the Netherlands; Department of Orthopedics, University Medical Center Utrecht, Utrecht, the NetherlandsDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the NetherlandsDepartment of Internal Medicine, Erasmus Medical Center, Rotterdam, the NetherlandsAdditive Manufacturing Laboratory, Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, the NetherlandsAdditive Manufacturing Laboratory, Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, the NetherlandsAdditive Manufacturing Laboratory, Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, the NetherlandsThe holy grail of orthopedic implant design is to ward off both aseptic and septic loosening for long enough that the implant outlives the patient. Questing this holy grail is feasible only if orthopedic biomaterials possess a long list of functionalities that enable them to discharge the onerous task of permanently replacing the native bone tissue. Here, we present a rationally designed and additive manufacturing (AM) topologically ordered porous metallic biomaterial that is made from Ti-6Al-4V using selective laser melting and packs most (if not all) of the required functionalities into a single implant. In addition to presenting a fully interconnected porous structure and form-freedom that enables realization of patient-specific implants, the biomaterials developed here were biofunctionalized using plasma electrolytic oxidation to locally release both osteogenic (i.e. strontium) and antibacterial (i.e. silver ions) agents. The same single-step biofunctionalization process also incorporated hydroxyapatite into the surface of the implants. Our measurements verified the continued release of both types of active agents up to 28 days. Assessment of the antibacterial activity in vitro and in an ex vivo murine model demonstrated extraordinarily high levels of bactericidal effects against a highly virulent and multidrug-resistant Staphylococcus aureus strain (i.e. USA300) with total eradication of both planktonic and adherent bacteria. This strong antibacterial behavior was combined with a significantly enhanced osteogenic behavior, as evidenced by significantly higher levels of alkaline phosphatase (ALP) activity compared with non-biofunctionalized implants. Finally, we discovered synergistic antibacterial behavior between strontium and silver ions, meaning that 4–32 folds lower concentrations of silver ions were required to achieve growth inhibition and total killing of bacteria. The functionality-packed biomaterial presented here demonstrates a unique combination of functionalities that make it an advanced prototype of future orthopedic biomaterials where implants will outlive patients.http://www.sciencedirect.com/science/article/pii/S259000642030020XMultifunctional surfacesBiofunctionalizationAntimicrobial implantAdditive manufacturingStrontiumSilver nanoparticles |
| spellingShingle | I.A.J. van Hengel F.S.A. Gelderman S. Athanasiadis M. Minneboo H. Weinans A.C. Fluit B.C.J. van der Eerden L.E. Fratila-Apachitei I. Apachitei A.A. Zadpoor Functionality-packed additively manufactured porous titanium implants Materials Today Bio Multifunctional surfaces Biofunctionalization Antimicrobial implant Additive manufacturing Strontium Silver nanoparticles |
| title | Functionality-packed additively manufactured porous titanium implants |
| title_full | Functionality-packed additively manufactured porous titanium implants |
| title_fullStr | Functionality-packed additively manufactured porous titanium implants |
| title_full_unstemmed | Functionality-packed additively manufactured porous titanium implants |
| title_short | Functionality-packed additively manufactured porous titanium implants |
| title_sort | functionality packed additively manufactured porous titanium implants |
| topic | Multifunctional surfaces Biofunctionalization Antimicrobial implant Additive manufacturing Strontium Silver nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S259000642030020X |
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