IGF-1 rs6218 polymorphisms modulate the susceptibility to age-related cataract
Background Single nucleotide polymorphisms (SNPs), as the most abundant form of DNA variation in the human genome, contribute to age-related cataracts (ARC) development. Apoptosis of lens epithelial cells (LECs) is closely related to ARC formation. Insulin-like growth factor 1 (IGF1) contributes to...
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PeerJ Inc.
2024-04-01
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| Online Access: | https://peerj.com/articles/17220.pdf |
| _version_ | 1797214189719126016 |
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| author | Xi Zou Jun Zhang Yong Wang Dong Zhou Guohua Deng Zhinan Liu |
| author_facet | Xi Zou Jun Zhang Yong Wang Dong Zhou Guohua Deng Zhinan Liu |
| author_sort | Xi Zou |
| collection | DOAJ |
| description | Background Single nucleotide polymorphisms (SNPs), as the most abundant form of DNA variation in the human genome, contribute to age-related cataracts (ARC) development. Apoptosis of lens epithelial cells (LECs) is closely related to ARC formation. Insulin-like growth factor 1 (IGF1) contributes to cell apoptosis regulation. Moreover, IGF1 was indicated to exhibit a close association with cataract formation. Afterward, an investigation was conducted to examine the correlation between polymorphisms in IGF1 and the susceptibility to ARC. Methods The present investigation was a case-control study. Venous blood draws were collected from the participants for DNA genotyping. Lens capsule samples were collected to detect mRNA and apoptosis. TaqMan RT-PCR was used to detect IGF1 polymorphism genotypes and qRT PCR was used to detect IGF1 mRNA levels in LECs. LEC apoptosis was evaluated through flow cytometry. The chi-square test was used to compare differences between ARCs and controls of each SNP. Results We found that the G allele frequency in the IGF1-rs6218 was higher in the ARCs than in the controls. Furthermore, it was observed that the rs6218 GG genotype exhibited a positive correlation to elevated levels of IGF1 mRNA in LECs. The IGF1 mRNA in the LECs and the apoptosis of LECs in nuclear type of ARCs (ARNC) was higher than the controls. Conclusion The susceptibility to ARC was related to IGF1-rs6218 polymorphism, and this polymorphism is associated with IGF1 expression at the mRNA level. Moreover, apoptosis in LECs of ARNCs was found to be increased. |
| first_indexed | 2024-04-24T11:10:13Z |
| format | Article |
| id | doaj.art-0132deaaefb146f2a17e803781b4d670 |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-04-24T11:10:13Z |
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| spelling | doaj.art-0132deaaefb146f2a17e803781b4d6702024-04-11T15:05:08ZengPeerJ Inc.PeerJ2167-83592024-04-0112e1722010.7717/peerj.17220IGF-1 rs6218 polymorphisms modulate the susceptibility to age-related cataractXi Zou0Jun Zhang1Yong Wang2Dong Zhou3Guohua Deng4Zhinan Liu5Changzhou Medical Center, Changzhou, ChinaThe Third People’s Hospital of Changzhou, Changzhou, ChinaNantong First People’s Hospital, Nantong, ChinaThe Third People’s Hospital of Changzhou, Changzhou, ChinaThe Third People’s Hospital of Changzhou, Changzhou, ChinaThe Third People’s Hospital of Changzhou, Changzhou, ChinaBackground Single nucleotide polymorphisms (SNPs), as the most abundant form of DNA variation in the human genome, contribute to age-related cataracts (ARC) development. Apoptosis of lens epithelial cells (LECs) is closely related to ARC formation. Insulin-like growth factor 1 (IGF1) contributes to cell apoptosis regulation. Moreover, IGF1 was indicated to exhibit a close association with cataract formation. Afterward, an investigation was conducted to examine the correlation between polymorphisms in IGF1 and the susceptibility to ARC. Methods The present investigation was a case-control study. Venous blood draws were collected from the participants for DNA genotyping. Lens capsule samples were collected to detect mRNA and apoptosis. TaqMan RT-PCR was used to detect IGF1 polymorphism genotypes and qRT PCR was used to detect IGF1 mRNA levels in LECs. LEC apoptosis was evaluated through flow cytometry. The chi-square test was used to compare differences between ARCs and controls of each SNP. Results We found that the G allele frequency in the IGF1-rs6218 was higher in the ARCs than in the controls. Furthermore, it was observed that the rs6218 GG genotype exhibited a positive correlation to elevated levels of IGF1 mRNA in LECs. The IGF1 mRNA in the LECs and the apoptosis of LECs in nuclear type of ARCs (ARNC) was higher than the controls. Conclusion The susceptibility to ARC was related to IGF1-rs6218 polymorphism, and this polymorphism is associated with IGF1 expression at the mRNA level. Moreover, apoptosis in LECs of ARNCs was found to be increased.https://peerj.com/articles/17220.pdfAge-related cataract (ARC)Lens epithelial cells (LECs)Single nucleotide polymorphisms (SNPs)Insulin-like growth factor 1(IGF1)Apoptosis |
| spellingShingle | Xi Zou Jun Zhang Yong Wang Dong Zhou Guohua Deng Zhinan Liu IGF-1 rs6218 polymorphisms modulate the susceptibility to age-related cataract PeerJ Age-related cataract (ARC) Lens epithelial cells (LECs) Single nucleotide polymorphisms (SNPs) Insulin-like growth factor 1(IGF1) Apoptosis |
| title | IGF-1 rs6218 polymorphisms modulate the susceptibility to age-related cataract |
| title_full | IGF-1 rs6218 polymorphisms modulate the susceptibility to age-related cataract |
| title_fullStr | IGF-1 rs6218 polymorphisms modulate the susceptibility to age-related cataract |
| title_full_unstemmed | IGF-1 rs6218 polymorphisms modulate the susceptibility to age-related cataract |
| title_short | IGF-1 rs6218 polymorphisms modulate the susceptibility to age-related cataract |
| title_sort | igf 1 rs6218 polymorphisms modulate the susceptibility to age related cataract |
| topic | Age-related cataract (ARC) Lens epithelial cells (LECs) Single nucleotide polymorphisms (SNPs) Insulin-like growth factor 1(IGF1) Apoptosis |
| url | https://peerj.com/articles/17220.pdf |
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