Effect of lactose, skim milk and Tris diluents on frozen buffalo spermatozoa
The composition of the extender in which semen is diluted before freezing plays a major role in successful cryopreservation of spermatozoa. This study was carried out to identify the suitable buffer for cryopreservation of buffalo semen. Sixteen split pooled ejaculates from two buffalo bulls possess...
| Main Authors: | , |
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| Format: | Article |
| Language: | fas |
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Islamic Azad University, Tabriz Branch
2007-08-01
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| Series: | Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī |
| Subjects: | |
| Online Access: | http://jvcp.iaut.ac.ir/article_518625_52c6b52033d9fc3f91b6202ad2f5d8dc.pdf |
| _version_ | 1828294790257573888 |
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| author | Ali Rastegarnia V Shafepour |
| author_facet | Ali Rastegarnia V Shafepour |
| author_sort | Ali Rastegarnia |
| collection | DOAJ |
| description | The composition of the extender in which semen is diluted before freezing plays a major role in successful cryopreservation of spermatozoa. This study was carried out to identify the suitable buffer for cryopreservation of buffalo semen. Sixteen split pooled ejaculates from two buffalo bulls possessing more than 70% visual sperm motility, were diluted at 370c either in lactose, skim milk or Tris extenders. The diluted semen was cooled to 40c within 2 hours, equilibrated at 40c for 4-6 hours following the addition of glycerol, filled in 0.5 ml French straws and frozen in a programmable cell freezer before plunging into liquid nitrogen. Semen was thawed at 370c for 30 seconds after 48 hours of storage inside liquid nitrogen. Post thaw visual sperm motility, plasma membrane integrity and acrosome morphology of each semen sample were assessed by warm plate microscopy at 370c, hypo-osmotic swelling test (HOST) and giemsa staining, respectively. Analysis of variance revelated that percentage of post thaw visual sperm motility (Mean± standard deviation) tended to be higher in Tris (50±3.6) than skim milk (44.5±2.5) and lower in lactose (24.4±10.5) extenders (P |
| first_indexed | 2024-04-13T11:43:30Z |
| format | Article |
| id | doaj.art-0132fdb916524668bdd4e7d3760d7ac8 |
| institution | Directory Open Access Journal |
| issn | 2322-4746 2476-6984 |
| language | fas |
| last_indexed | 2024-04-13T11:43:30Z |
| publishDate | 2007-08-01 |
| publisher | Islamic Azad University, Tabriz Branch |
| record_format | Article |
| series | Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī |
| spelling | doaj.art-0132fdb916524668bdd4e7d3760d7ac82022-12-22T02:48:14ZfasIslamic Azad University, Tabriz BranchĀsīb/shināsī-i Darmāngāhī-i Dāmpizishkī2322-47462476-69842007-08-0112 (2) تابستان7988518625Effect of lactose, skim milk and Tris diluents on frozen buffalo spermatozoaAli Rastegarnia0V Shafepour1Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University of Urmia, Urmia, IranBuffalo Breeding and Extension Training Center, Urmia, Urmia, IranThe composition of the extender in which semen is diluted before freezing plays a major role in successful cryopreservation of spermatozoa. This study was carried out to identify the suitable buffer for cryopreservation of buffalo semen. Sixteen split pooled ejaculates from two buffalo bulls possessing more than 70% visual sperm motility, were diluted at 370c either in lactose, skim milk or Tris extenders. The diluted semen was cooled to 40c within 2 hours, equilibrated at 40c for 4-6 hours following the addition of glycerol, filled in 0.5 ml French straws and frozen in a programmable cell freezer before plunging into liquid nitrogen. Semen was thawed at 370c for 30 seconds after 48 hours of storage inside liquid nitrogen. Post thaw visual sperm motility, plasma membrane integrity and acrosome morphology of each semen sample were assessed by warm plate microscopy at 370c, hypo-osmotic swelling test (HOST) and giemsa staining, respectively. Analysis of variance revelated that percentage of post thaw visual sperm motility (Mean± standard deviation) tended to be higher in Tris (50±3.6) than skim milk (44.5±2.5) and lower in lactose (24.4±10.5) extenders (Phttp://jvcp.iaut.ac.ir/article_518625_52c6b52033d9fc3f91b6202ad2f5d8dc.pdfExtenderSpermatozoaBuffalo |
| spellingShingle | Ali Rastegarnia V Shafepour Effect of lactose, skim milk and Tris diluents on frozen buffalo spermatozoa Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī Extender Spermatozoa Buffalo |
| title | Effect of lactose, skim milk and Tris diluents on frozen buffalo spermatozoa |
| title_full | Effect of lactose, skim milk and Tris diluents on frozen buffalo spermatozoa |
| title_fullStr | Effect of lactose, skim milk and Tris diluents on frozen buffalo spermatozoa |
| title_full_unstemmed | Effect of lactose, skim milk and Tris diluents on frozen buffalo spermatozoa |
| title_short | Effect of lactose, skim milk and Tris diluents on frozen buffalo spermatozoa |
| title_sort | effect of lactose skim milk and tris diluents on frozen buffalo spermatozoa |
| topic | Extender Spermatozoa Buffalo |
| url | http://jvcp.iaut.ac.ir/article_518625_52c6b52033d9fc3f91b6202ad2f5d8dc.pdf |
| work_keys_str_mv | AT alirastegarnia effectoflactoseskimmilkandtrisdiluentsonfrozenbuffalospermatozoa AT vshafepour effectoflactoseskimmilkandtrisdiluentsonfrozenbuffalospermatozoa |