Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions
Abstract The mouse is the most important mammalian model in life science research and the behavior of the mouse is a key read-out of experimental interventions and genetic manipulations. To serve this purpose a solid understanding of the mouse normal behavior is a prerequisite. Using 14–19 months of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-84141-9 |
| _version_ | 1818977771543592960 |
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| author | Karin Pernold Eric Rullman Brun Ulfhake |
| author_facet | Karin Pernold Eric Rullman Brun Ulfhake |
| author_sort | Karin Pernold |
| collection | DOAJ |
| description | Abstract The mouse is the most important mammalian model in life science research and the behavior of the mouse is a key read-out of experimental interventions and genetic manipulations. To serve this purpose a solid understanding of the mouse normal behavior is a prerequisite. Using 14–19 months of cumulative 24/7 home-cage activity recorded with a non-intrusive technique, evidence is here provided for a highly significant circannual oscillation in spontaneous activity (1–2 SD of the mean, on average 65% higher during peak of highs than lows; P = 7E−50) of male and female C57BL/6 mice held under constant conditions. The periodicity of this hitherto not recognized oscillation is in the range of 2–4 months (average estimate was 97 days across cohorts of cages). It off-sets responses to environmental stimuli and co-varies with the feeding behavior but does not significantly alter the preference for being active during the dark hours. The absence of coordination of this rhythmicity between cages with mice or seasons of the year suggest that the oscillation of physical activity is generated by a free-running intrinsic oscillator devoid of external timer. Due to the magnitude of this rhythmic variation it may be a serious confounder in experiments on mice if left unrecognized. |
| first_indexed | 2024-12-20T16:33:02Z |
| format | Article |
| id | doaj.art-0133101a7c794ac68e523eef0dd24c5b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T16:33:02Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0133101a7c794ac68e523eef0dd24c5b2022-12-21T19:33:13ZengNature PortfolioScientific Reports2045-23222021-03-0111111310.1038/s41598-021-84141-9Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditionsKarin Pernold0Eric Rullman1Brun Ulfhake2Division Clinical Physiology, Department of Laboratory Medicine, Karolinska InstitutetDivision Clinical Physiology, Department of Laboratory Medicine, Karolinska InstitutetDivision Clinical Physiology, Department of Laboratory Medicine, Karolinska InstitutetAbstract The mouse is the most important mammalian model in life science research and the behavior of the mouse is a key read-out of experimental interventions and genetic manipulations. To serve this purpose a solid understanding of the mouse normal behavior is a prerequisite. Using 14–19 months of cumulative 24/7 home-cage activity recorded with a non-intrusive technique, evidence is here provided for a highly significant circannual oscillation in spontaneous activity (1–2 SD of the mean, on average 65% higher during peak of highs than lows; P = 7E−50) of male and female C57BL/6 mice held under constant conditions. The periodicity of this hitherto not recognized oscillation is in the range of 2–4 months (average estimate was 97 days across cohorts of cages). It off-sets responses to environmental stimuli and co-varies with the feeding behavior but does not significantly alter the preference for being active during the dark hours. The absence of coordination of this rhythmicity between cages with mice or seasons of the year suggest that the oscillation of physical activity is generated by a free-running intrinsic oscillator devoid of external timer. Due to the magnitude of this rhythmic variation it may be a serious confounder in experiments on mice if left unrecognized.https://doi.org/10.1038/s41598-021-84141-9 |
| spellingShingle | Karin Pernold Eric Rullman Brun Ulfhake Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions Scientific Reports |
| title | Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions |
| title_full | Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions |
| title_fullStr | Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions |
| title_full_unstemmed | Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions |
| title_short | Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions |
| title_sort | major oscillations in spontaneous home cage activity in c57bl 6 mice housed under constant conditions |
| url | https://doi.org/10.1038/s41598-021-84141-9 |
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