Pathogenesis of Rift Valley Fever Virus Aerosol Infection in <i>STAT2</i> Knockout Hamsters
Rift Valley fever virus (RVFV) is an emerging pathogen capable of causing severe disease in livestock and humans and can be transmitted by multiple routes including aerosol exposure. Several animal models have been developed to gain insight into the pathogenesis associated with aerosolized RVFV infe...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-11-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/10/11/651 |
| _version_ | 1819275018629021696 |
|---|---|
| author | Brady T. Hickerson Jonna B. Westover Arnaud J. Van Wettere Johanna D. Rigas Jinxin Miao Bettina L. Conrad Neil E. Motter Zhongde Wang Brian B. Gowen |
| author_facet | Brady T. Hickerson Jonna B. Westover Arnaud J. Van Wettere Johanna D. Rigas Jinxin Miao Bettina L. Conrad Neil E. Motter Zhongde Wang Brian B. Gowen |
| author_sort | Brady T. Hickerson |
| collection | DOAJ |
| description | Rift Valley fever virus (RVFV) is an emerging pathogen capable of causing severe disease in livestock and humans and can be transmitted by multiple routes including aerosol exposure. Several animal models have been developed to gain insight into the pathogenesis associated with aerosolized RVFV infection, but work with these models is restricted to high containment biosafety level (BSL) laboratories limiting their use for antiviral and vaccine development studies. Here, we report on a new RVFV inhalation infection model in <i>STAT2</i> KO hamsters exposed to aerosolized MP-12 vaccine virus by nose-only inhalation that enables a more accurate delivery and measurement of exposure dose. RVFV was detected in hepatic and other tissues 4⁻5 days after challenge, consistent with virus-induced lesions in the liver, spleen and lung. Furthermore, assessment of blood chemistry and hematological parameters revealed alterations in several liver disease markers and white blood cell parameters. Our results indicate that <i>STAT2</i> KO hamsters develop a disease course that shares features of disease observed in human cases and in other animal models of RVFV aerosol exposure, supporting the use of this BSL-2 infection model for countermeasure development efforts. |
| first_indexed | 2024-12-23T23:17:39Z |
| format | Article |
| id | doaj.art-01331e20e29e452abba7fecf6a5fad84 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-23T23:17:39Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-01331e20e29e452abba7fecf6a5fad842022-12-21T17:26:27ZengMDPI AGViruses1999-49152018-11-01101165110.3390/v10110651v10110651Pathogenesis of Rift Valley Fever Virus Aerosol Infection in <i>STAT2</i> Knockout HamstersBrady T. Hickerson0Jonna B. Westover1Arnaud J. Van Wettere2Johanna D. Rigas3Jinxin Miao4Bettina L. Conrad5Neil E. Motter6Zhongde Wang7Brian B. Gowen8Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USADepartment of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USADepartment of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USAUtah Veterinary Diagnostic Laboratory, Logan, UT 84341, USADepartment of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USAUtah Veterinary Diagnostic Laboratory, Logan, UT 84341, USADepartment of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USADepartment of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USADepartment of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USARift Valley fever virus (RVFV) is an emerging pathogen capable of causing severe disease in livestock and humans and can be transmitted by multiple routes including aerosol exposure. Several animal models have been developed to gain insight into the pathogenesis associated with aerosolized RVFV infection, but work with these models is restricted to high containment biosafety level (BSL) laboratories limiting their use for antiviral and vaccine development studies. Here, we report on a new RVFV inhalation infection model in <i>STAT2</i> KO hamsters exposed to aerosolized MP-12 vaccine virus by nose-only inhalation that enables a more accurate delivery and measurement of exposure dose. RVFV was detected in hepatic and other tissues 4⁻5 days after challenge, consistent with virus-induced lesions in the liver, spleen and lung. Furthermore, assessment of blood chemistry and hematological parameters revealed alterations in several liver disease markers and white blood cell parameters. Our results indicate that <i>STAT2</i> KO hamsters develop a disease course that shares features of disease observed in human cases and in other animal models of RVFV aerosol exposure, supporting the use of this BSL-2 infection model for countermeasure development efforts.https://www.mdpi.com/1999-4915/10/11/651Rift Valley fever virusbunyavirusphlebovirusaerosol<i>STAT2</i>interferon |
| spellingShingle | Brady T. Hickerson Jonna B. Westover Arnaud J. Van Wettere Johanna D. Rigas Jinxin Miao Bettina L. Conrad Neil E. Motter Zhongde Wang Brian B. Gowen Pathogenesis of Rift Valley Fever Virus Aerosol Infection in <i>STAT2</i> Knockout Hamsters Viruses Rift Valley fever virus bunyavirus phlebovirus aerosol <i>STAT2</i> interferon |
| title | Pathogenesis of Rift Valley Fever Virus Aerosol Infection in <i>STAT2</i> Knockout Hamsters |
| title_full | Pathogenesis of Rift Valley Fever Virus Aerosol Infection in <i>STAT2</i> Knockout Hamsters |
| title_fullStr | Pathogenesis of Rift Valley Fever Virus Aerosol Infection in <i>STAT2</i> Knockout Hamsters |
| title_full_unstemmed | Pathogenesis of Rift Valley Fever Virus Aerosol Infection in <i>STAT2</i> Knockout Hamsters |
| title_short | Pathogenesis of Rift Valley Fever Virus Aerosol Infection in <i>STAT2</i> Knockout Hamsters |
| title_sort | pathogenesis of rift valley fever virus aerosol infection in i stat2 i knockout hamsters |
| topic | Rift Valley fever virus bunyavirus phlebovirus aerosol <i>STAT2</i> interferon |
| url | https://www.mdpi.com/1999-4915/10/11/651 |
| work_keys_str_mv | AT bradythickerson pathogenesisofriftvalleyfevervirusaerosolinfectioninistat2iknockouthamsters AT jonnabwestover pathogenesisofriftvalleyfevervirusaerosolinfectioninistat2iknockouthamsters AT arnaudjvanwettere pathogenesisofriftvalleyfevervirusaerosolinfectioninistat2iknockouthamsters AT johannadrigas pathogenesisofriftvalleyfevervirusaerosolinfectioninistat2iknockouthamsters AT jinxinmiao pathogenesisofriftvalleyfevervirusaerosolinfectioninistat2iknockouthamsters AT bettinalconrad pathogenesisofriftvalleyfevervirusaerosolinfectioninistat2iknockouthamsters AT neilemotter pathogenesisofriftvalleyfevervirusaerosolinfectioninistat2iknockouthamsters AT zhongdewang pathogenesisofriftvalleyfevervirusaerosolinfectioninistat2iknockouthamsters AT brianbgowen pathogenesisofriftvalleyfevervirusaerosolinfectioninistat2iknockouthamsters |