Effects of Rumen-Protected Creatine Pyruvate on Meat Quality, Hepatic Gluconeogenesis, and Muscle Energy Metabolism of Long-Distance Transported Beef Cattle
Pre-slaughter long-distance transport resulted in a rapid depletion of muscle glycogen and led to a higher rate of dark, firm and dry (DFD) meat. Therefore, enhancing muscle glycogen reserves is critical for beef cattle prior to transportation. Creatine pyruvate (CrPyr) can provide simultaneous pyru...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Animal Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fanim.2022.904503/full |
| _version_ | 1811249902612643840 |
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| author | Guwei Lu Yanjiao Li Kang Mao Yitian Zang Xianghui Zhao Qinghua Qiu Mingren Qu Kehui Ouyang |
| author_facet | Guwei Lu Yanjiao Li Kang Mao Yitian Zang Xianghui Zhao Qinghua Qiu Mingren Qu Kehui Ouyang |
| author_sort | Guwei Lu |
| collection | DOAJ |
| description | Pre-slaughter long-distance transport resulted in a rapid depletion of muscle glycogen and led to a higher rate of dark, firm and dry (DFD) meat. Therefore, enhancing muscle glycogen reserves is critical for beef cattle prior to transportation. Creatine pyruvate (CrPyr) can provide simultaneous pyruvate and creatine and both are proven to promote the glycogen reserves. This study aimed to investigate the effects of transport treatment and dietary supplementation of rumen-protected (RP)-CrPyr on the meat quality, muscle energy metabolism, and hepatic gluconeogenesis of beef cattle. Twenty 18 month-old male Simmental crossbred cattle (659 ± 16 kg) were allotted 4 treatments based on a 2 × 2 factorial arrangement with two RP-CrPyr levels (140 g/d or 0 g/d) and two transport treatments (12 h or 5 min): ST_CrPyr0, ST_CrPyr140, LT_CrPyr0 and LT_CrPyr140. Three cattle per group were slaughtered after 30 days of feeding. The interaction of transport and RP-CrPyr had a significant effect on the muscle pH45 min, redness, glycogen content, GP, and AMP level (P < 0.05). Compared with short-distance transport, long-distance transport increased the muscle pH45 min value, redness, yellowness, drip loss, creatine level (P < 0.05), decreased muscle glycogen content, glycolytic potential (GP), and liver glucose amount (P < 0.05). Supplementation of RP-CrPyr decreased the activities of creatine kinase and lactate dehydrogenase in serum, muscle pH24 h value, redness, yellowness, lactate content, AMP level, and AMP/ATP (P < 0.05), increased the muscle glycogen content, GP, hexokinase activity, ATP and ADP levels, and ATP/ADP, liver pyruvate and glucose contents, activity of pyruvate carboxylase in the liver of cattle than those in the nonsupplemented treatments (P < 0.05). These results indicated that dietary RP-CrPyr supplementation might be favorable to improve meat quality and regulatory capacity of energy metabolism of beef cattle suffering long-distance transport followed with recovery treatment by increasing muscle glycogen storage, energy supply, and hepatic gluconeogenesis. |
| first_indexed | 2024-04-12T15:55:41Z |
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| id | doaj.art-013373ea7bb04e39a052f722bcc74f2f |
| institution | Directory Open Access Journal |
| issn | 2673-6225 |
| language | English |
| last_indexed | 2024-04-12T15:55:41Z |
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| series | Frontiers in Animal Science |
| spelling | doaj.art-013373ea7bb04e39a052f722bcc74f2f2022-12-22T03:26:22ZengFrontiers Media S.A.Frontiers in Animal Science2673-62252022-06-01310.3389/fanim.2022.904503904503Effects of Rumen-Protected Creatine Pyruvate on Meat Quality, Hepatic Gluconeogenesis, and Muscle Energy Metabolism of Long-Distance Transported Beef CattleGuwei Lu0Yanjiao Li1Kang Mao2Yitian Zang3Xianghui Zhao4Qinghua Qiu5Mingren Qu6Kehui Ouyang7Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Animal Nutrition and Feed Safety Innovation Team, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, ChinaJiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Animal Nutrition and Feed Safety Innovation Team, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Animal Nutrition and Feed Safety Innovation Team, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, ChinaJiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Animal Nutrition and Feed Safety Innovation Team, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, ChinaPre-slaughter long-distance transport resulted in a rapid depletion of muscle glycogen and led to a higher rate of dark, firm and dry (DFD) meat. Therefore, enhancing muscle glycogen reserves is critical for beef cattle prior to transportation. Creatine pyruvate (CrPyr) can provide simultaneous pyruvate and creatine and both are proven to promote the glycogen reserves. This study aimed to investigate the effects of transport treatment and dietary supplementation of rumen-protected (RP)-CrPyr on the meat quality, muscle energy metabolism, and hepatic gluconeogenesis of beef cattle. Twenty 18 month-old male Simmental crossbred cattle (659 ± 16 kg) were allotted 4 treatments based on a 2 × 2 factorial arrangement with two RP-CrPyr levels (140 g/d or 0 g/d) and two transport treatments (12 h or 5 min): ST_CrPyr0, ST_CrPyr140, LT_CrPyr0 and LT_CrPyr140. Three cattle per group were slaughtered after 30 days of feeding. The interaction of transport and RP-CrPyr had a significant effect on the muscle pH45 min, redness, glycogen content, GP, and AMP level (P < 0.05). Compared with short-distance transport, long-distance transport increased the muscle pH45 min value, redness, yellowness, drip loss, creatine level (P < 0.05), decreased muscle glycogen content, glycolytic potential (GP), and liver glucose amount (P < 0.05). Supplementation of RP-CrPyr decreased the activities of creatine kinase and lactate dehydrogenase in serum, muscle pH24 h value, redness, yellowness, lactate content, AMP level, and AMP/ATP (P < 0.05), increased the muscle glycogen content, GP, hexokinase activity, ATP and ADP levels, and ATP/ADP, liver pyruvate and glucose contents, activity of pyruvate carboxylase in the liver of cattle than those in the nonsupplemented treatments (P < 0.05). These results indicated that dietary RP-CrPyr supplementation might be favorable to improve meat quality and regulatory capacity of energy metabolism of beef cattle suffering long-distance transport followed with recovery treatment by increasing muscle glycogen storage, energy supply, and hepatic gluconeogenesis.https://www.frontiersin.org/articles/10.3389/fanim.2022.904503/fullbeef cattlecreatine pyruvatemeat qualitymuscle glycogentransport stress |
| spellingShingle | Guwei Lu Yanjiao Li Kang Mao Yitian Zang Xianghui Zhao Qinghua Qiu Mingren Qu Kehui Ouyang Effects of Rumen-Protected Creatine Pyruvate on Meat Quality, Hepatic Gluconeogenesis, and Muscle Energy Metabolism of Long-Distance Transported Beef Cattle Frontiers in Animal Science beef cattle creatine pyruvate meat quality muscle glycogen transport stress |
| title | Effects of Rumen-Protected Creatine Pyruvate on Meat Quality, Hepatic Gluconeogenesis, and Muscle Energy Metabolism of Long-Distance Transported Beef Cattle |
| title_full | Effects of Rumen-Protected Creatine Pyruvate on Meat Quality, Hepatic Gluconeogenesis, and Muscle Energy Metabolism of Long-Distance Transported Beef Cattle |
| title_fullStr | Effects of Rumen-Protected Creatine Pyruvate on Meat Quality, Hepatic Gluconeogenesis, and Muscle Energy Metabolism of Long-Distance Transported Beef Cattle |
| title_full_unstemmed | Effects of Rumen-Protected Creatine Pyruvate on Meat Quality, Hepatic Gluconeogenesis, and Muscle Energy Metabolism of Long-Distance Transported Beef Cattle |
| title_short | Effects of Rumen-Protected Creatine Pyruvate on Meat Quality, Hepatic Gluconeogenesis, and Muscle Energy Metabolism of Long-Distance Transported Beef Cattle |
| title_sort | effects of rumen protected creatine pyruvate on meat quality hepatic gluconeogenesis and muscle energy metabolism of long distance transported beef cattle |
| topic | beef cattle creatine pyruvate meat quality muscle glycogen transport stress |
| url | https://www.frontiersin.org/articles/10.3389/fanim.2022.904503/full |
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