Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update
Abstract Background The Borrelia burgdorferi sensu lato (s.l.) genogroup is the causative agent responsible for Lyme borreliosis, a common tick-borne infectious disease in some temperate regions of the Northern Hemisphere. In humans, the clinical manifestations of Lyme borreliosis vary from dermatol...
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-021-05099-1 |
| _version_ | 1829465074892275712 |
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| author | Zsuzsa Kalmár Violeta Briciu Mircea Coroian Mirela Flonta Amanda-Lelia Rădulescu Adriana Topan Andrei Daniel Mihalca Mihaela Lupșe |
| author_facet | Zsuzsa Kalmár Violeta Briciu Mircea Coroian Mirela Flonta Amanda-Lelia Rădulescu Adriana Topan Andrei Daniel Mihalca Mihaela Lupșe |
| author_sort | Zsuzsa Kalmár |
| collection | DOAJ |
| description | Abstract Background The Borrelia burgdorferi sensu lato (s.l.) genogroup is the causative agent responsible for Lyme borreliosis, a common tick-borne infectious disease in some temperate regions of the Northern Hemisphere. In humans, the clinical manifestations of Lyme borreliosis vary from dermatological infection to severe systemic manifestations. In Romania, data on the seroprevalence of Lyme borreliosis and associated risk factors are scarce and outdated, as the only seroprevalence study with a large dataset was published more than 20 years ago. Therefore, the aim of the present study was to evaluate the seroprevalence for Borrelia burgdorferi s.l. in healthy blood donors from six Romanian counties and identify the associated risk factors. Methods The study was conducted among 1200 healthy blood donors aged between 18 and 65 years during November 2019 and September 2020 from six counties in the northwestern and central parts of Romania. A two-tiered testing strategy was applied. Positive and equivocal immunoenzymatic test results for IgG and IgM antibodies were further confirmed by Western blot. Results Serum samples from 20% of the blood donors had positive or equivocal IgG and IgM ELISA index values. In total, 2.3% of the serum samples for IgG and 1.8% for IgM were positive by Western blot. The seroprevalence for both antibodies varied between 1.5% (Satu-Mare) and 6.5% (Bistrița-Năsăud) in the six counties investigated. The highest seroprevalence was observed in men (4.7%), in blood donors performing their professional activities outdoors (4.2%), and in those aged ≥ 56 years (8%). Conclusions These findings confirm the presence of specific IgG and IgM antibodies to B. burgdorferi s.l. among healthy blood donors from Romania. Furthermore, potential risk factors, such as gender, age, and behavior, associated with the presence of positive B. burgdorferi s.l. antibodies among healthy blood donors were identified. Graphical Abstract |
| first_indexed | 2024-12-13T16:48:12Z |
| format | Article |
| id | doaj.art-0134d86374a24303ae241406afa0126a |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-13T16:48:12Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-0134d86374a24303ae241406afa0126a2022-12-21T23:38:06ZengBMCParasites & Vectors1756-33052021-12-0114111010.1186/s13071-021-05099-1Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an updateZsuzsa Kalmár0Violeta Briciu1Mircea Coroian2Mirela Flonta3Amanda-Lelia Rădulescu4Adriana Topan5Andrei Daniel Mihalca6Mihaela Lupșe7“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-NapocaHospital for Infectious Diseases“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine Cluj-Napoca“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-NapocaAbstract Background The Borrelia burgdorferi sensu lato (s.l.) genogroup is the causative agent responsible for Lyme borreliosis, a common tick-borne infectious disease in some temperate regions of the Northern Hemisphere. In humans, the clinical manifestations of Lyme borreliosis vary from dermatological infection to severe systemic manifestations. In Romania, data on the seroprevalence of Lyme borreliosis and associated risk factors are scarce and outdated, as the only seroprevalence study with a large dataset was published more than 20 years ago. Therefore, the aim of the present study was to evaluate the seroprevalence for Borrelia burgdorferi s.l. in healthy blood donors from six Romanian counties and identify the associated risk factors. Methods The study was conducted among 1200 healthy blood donors aged between 18 and 65 years during November 2019 and September 2020 from six counties in the northwestern and central parts of Romania. A two-tiered testing strategy was applied. Positive and equivocal immunoenzymatic test results for IgG and IgM antibodies were further confirmed by Western blot. Results Serum samples from 20% of the blood donors had positive or equivocal IgG and IgM ELISA index values. In total, 2.3% of the serum samples for IgG and 1.8% for IgM were positive by Western blot. The seroprevalence for both antibodies varied between 1.5% (Satu-Mare) and 6.5% (Bistrița-Năsăud) in the six counties investigated. The highest seroprevalence was observed in men (4.7%), in blood donors performing their professional activities outdoors (4.2%), and in those aged ≥ 56 years (8%). Conclusions These findings confirm the presence of specific IgG and IgM antibodies to B. burgdorferi s.l. among healthy blood donors from Romania. Furthermore, potential risk factors, such as gender, age, and behavior, associated with the presence of positive B. burgdorferi s.l. antibodies among healthy blood donors were identified. Graphical Abstracthttps://doi.org/10.1186/s13071-021-05099-1Lyme diseaseSeroprevalenceBorrelia burgdorferiAntibodiesIxodes ricinus |
| spellingShingle | Zsuzsa Kalmár Violeta Briciu Mircea Coroian Mirela Flonta Amanda-Lelia Rădulescu Adriana Topan Andrei Daniel Mihalca Mihaela Lupșe Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update Parasites & Vectors Lyme disease Seroprevalence Borrelia burgdorferi Antibodies Ixodes ricinus |
| title | Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update |
| title_full | Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update |
| title_fullStr | Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update |
| title_full_unstemmed | Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update |
| title_short | Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update |
| title_sort | seroprevalence of antibodies against borrelia burgdorferi sensu lato in healthy blood donors in romania an update |
| topic | Lyme disease Seroprevalence Borrelia burgdorferi Antibodies Ixodes ricinus |
| url | https://doi.org/10.1186/s13071-021-05099-1 |
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