| Summary: | In order to select excellent strains with high CO2 fixation capability on a large scale, nine Spirulina species were cultivated in columnar photobioreactors with the addition of 10% CO2. The two species selected (208 and 220) were optimized for pH value, total dissolved inorganic carbon (DIC), and phosphorus content with intermittent CO2 addition in 4 m2 indoor raceway ponds. On the basis of biomass accumulation and CO2 fixation rate in the present study, the optimum pH, DIC, and phosphate concentration were 9.5, 0.1 mol L−1, and 200 mg L−1 for both strains, respectively. Lastly, the two strains selected were semi-continuously cultivated successfully for CO2 mitigation in 605 m2 raceway ponds aerated with food-grade CO2 purified from a coal chemical flue gas on a large scale. The daily average biomass dry weight of the two stains reached up to 18.7 and 13.2 g m−2 d−1, respectively, suggesting the two Spirulina strains can be utilized for mass production.
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