Vascular reconstruction of the decellularized biomatrix for whole-organ engineering—a critical perspective and future strategies
Whole-organ re-engineering is the most challenging goal yet to be achieved in tissue engineering and regenerative medicine. One essential factor in any transplantable and functional tissue engineering is fabricating a perfusable vascular network with macro- and micro-sized blood vessels. Whole-organ...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1221159/full |
| _version_ | 1797628887130177536 |
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| author | Santosh Gupta Santosh Gupta Akriti Sharma Goran Petrovski Goran Petrovski Goran Petrovski Rama Shanker Verma |
| author_facet | Santosh Gupta Santosh Gupta Akriti Sharma Goran Petrovski Goran Petrovski Goran Petrovski Rama Shanker Verma |
| author_sort | Santosh Gupta |
| collection | DOAJ |
| description | Whole-organ re-engineering is the most challenging goal yet to be achieved in tissue engineering and regenerative medicine. One essential factor in any transplantable and functional tissue engineering is fabricating a perfusable vascular network with macro- and micro-sized blood vessels. Whole-organ development has become more practical with the use of the decellularized organ biomatrix (DOB) as it provides a native biochemical and structural framework for a particular organ. However, reconstructing vasculature and re-endothelialization in the DOB is a highly challenging task and has not been achieved for constructing a clinically transplantable vascularized organ with an efficient perfusable capability. Here, we critically and articulately emphasized factors that have been studied for the vascular reconstruction in the DOB. Furthermore, we highlighted the factors used for vasculature development studies in general and their application in whole-organ vascular reconstruction. We also analyzed in detail the strategies explored so far for vascular reconstruction and angiogenesis in the DOB for functional and perfusable vasculature development. Finally, we discussed some of the crucial factors that have been largely ignored in the vascular reconstruction of the DOB and the future directions that should be addressed systematically. |
| first_indexed | 2024-03-11T10:45:33Z |
| format | Article |
| id | doaj.art-01383828f3fd4da19a861d5cfbd7f640 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-11T10:45:33Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-01383828f3fd4da19a861d5cfbd7f6402023-11-14T04:57:38ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-11-011110.3389/fbioe.2023.12211591221159Vascular reconstruction of the decellularized biomatrix for whole-organ engineering—a critical perspective and future strategiesSantosh Gupta0Santosh Gupta1Akriti Sharma2Goran Petrovski3Goran Petrovski4Goran Petrovski5Rama Shanker Verma6Stem Cell and Molecular Biology, Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences. Indian Institute of Technology Madras, Chennai, IndiaCenter for Eye Research and Innovative Diagnostics, Department of Ophthalmology, Institute for Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, NorwayStem Cell and Molecular Biology, Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences. Indian Institute of Technology Madras, Chennai, IndiaCenter for Eye Research and Innovative Diagnostics, Department of Ophthalmology, Institute for Clinical Medicine, University of Oslo, Oslo, NorwayDepartment of Ophthalmology, Oslo University Hospital, Oslo, NorwayDepartment of Ophthalmology, University of Split School of Medicine and University Hospital Centre, Split, CroatiaStem Cell and Molecular Biology, Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences. Indian Institute of Technology Madras, Chennai, IndiaWhole-organ re-engineering is the most challenging goal yet to be achieved in tissue engineering and regenerative medicine. One essential factor in any transplantable and functional tissue engineering is fabricating a perfusable vascular network with macro- and micro-sized blood vessels. Whole-organ development has become more practical with the use of the decellularized organ biomatrix (DOB) as it provides a native biochemical and structural framework for a particular organ. However, reconstructing vasculature and re-endothelialization in the DOB is a highly challenging task and has not been achieved for constructing a clinically transplantable vascularized organ with an efficient perfusable capability. Here, we critically and articulately emphasized factors that have been studied for the vascular reconstruction in the DOB. Furthermore, we highlighted the factors used for vasculature development studies in general and their application in whole-organ vascular reconstruction. We also analyzed in detail the strategies explored so far for vascular reconstruction and angiogenesis in the DOB for functional and perfusable vasculature development. Finally, we discussed some of the crucial factors that have been largely ignored in the vascular reconstruction of the DOB and the future directions that should be addressed systematically.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1221159/fullvascular reconstructionartificial organwhole-organ engineeringdecellularized organendothelialization |
| spellingShingle | Santosh Gupta Santosh Gupta Akriti Sharma Goran Petrovski Goran Petrovski Goran Petrovski Rama Shanker Verma Vascular reconstruction of the decellularized biomatrix for whole-organ engineering—a critical perspective and future strategies Frontiers in Bioengineering and Biotechnology vascular reconstruction artificial organ whole-organ engineering decellularized organ endothelialization |
| title | Vascular reconstruction of the decellularized biomatrix for whole-organ engineering—a critical perspective and future strategies |
| title_full | Vascular reconstruction of the decellularized biomatrix for whole-organ engineering—a critical perspective and future strategies |
| title_fullStr | Vascular reconstruction of the decellularized biomatrix for whole-organ engineering—a critical perspective and future strategies |
| title_full_unstemmed | Vascular reconstruction of the decellularized biomatrix for whole-organ engineering—a critical perspective and future strategies |
| title_short | Vascular reconstruction of the decellularized biomatrix for whole-organ engineering—a critical perspective and future strategies |
| title_sort | vascular reconstruction of the decellularized biomatrix for whole organ engineering a critical perspective and future strategies |
| topic | vascular reconstruction artificial organ whole-organ engineering decellularized organ endothelialization |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1221159/full |
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