Cloning and Expression Analysis of Flavonoid 3′, 5′-Hydroxylase Gene from <i>Brunfelsia acuminata</i>
The full-length cDNA sequence of <i>F3′5′H</i> gene from the <i>Brunfelsia acuminata</i> was obtained by RT-PCR and RACE, whose GenBank accession number is JQ678765. The sequence contains a 1521 bp open reading frame, 120 bp 5′UTR and 61 bp 3′UTR, encoding a total of 506 amin...
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MDPI AG
2021-07-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/7/1086 |
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| author | Min Li Yuting Cao Biswojit Debnath Hongjuan Yang Xiaohua Kui Dongliang Qiu |
| author_facet | Min Li Yuting Cao Biswojit Debnath Hongjuan Yang Xiaohua Kui Dongliang Qiu |
| author_sort | Min Li |
| collection | DOAJ |
| description | The full-length cDNA sequence of <i>F3′5′H</i> gene from the <i>Brunfelsia acuminata</i> was obtained by RT-PCR and RACE, whose GenBank accession number is JQ678765. The sequence contains a 1521 bp open reading frame, 120 bp 5′UTR and 61 bp 3′UTR, encoding a total of 506 amino acids. The molecular mass of the predicted protein is 56.47 kDa with an estimated pI of 8.78, respectively. Sequence alignment showed that the amino acid sequence of F3′5′H was 91%, 87% and 84% with that of <i>Petunia × hybrida</i>, <i>Nierembergia sp</i>., <i>Solanum tuberosum</i>, respectively. Real-time quantitative PCR analysis showed that the expression of <i>F3′5′H</i> gene was different in petals of different days, which was the highest expression level on day 0 and significantly higher than other days. The results indicated that <i>F3′5′H</i> might play key role in flower color regulation and provide a theoretical reference for blue flower molecular breeding. |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T09:37:50Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-013cad5926d649b9baa5c8b415e6b5d62023-11-22T03:51:24ZengMDPI AGGenes2073-44252021-07-01127108610.3390/genes12071086Cloning and Expression Analysis of Flavonoid 3′, 5′-Hydroxylase Gene from <i>Brunfelsia acuminata</i>Min Li0Yuting Cao1Biswojit Debnath2Hongjuan Yang3Xiaohua Kui4Dongliang Qiu5College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaDepartment of Horticulture, Sylhet Agricultural University, Sylhet 3100, BangladeshCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThe full-length cDNA sequence of <i>F3′5′H</i> gene from the <i>Brunfelsia acuminata</i> was obtained by RT-PCR and RACE, whose GenBank accession number is JQ678765. The sequence contains a 1521 bp open reading frame, 120 bp 5′UTR and 61 bp 3′UTR, encoding a total of 506 amino acids. The molecular mass of the predicted protein is 56.47 kDa with an estimated pI of 8.78, respectively. Sequence alignment showed that the amino acid sequence of F3′5′H was 91%, 87% and 84% with that of <i>Petunia × hybrida</i>, <i>Nierembergia sp</i>., <i>Solanum tuberosum</i>, respectively. Real-time quantitative PCR analysis showed that the expression of <i>F3′5′H</i> gene was different in petals of different days, which was the highest expression level on day 0 and significantly higher than other days. The results indicated that <i>F3′5′H</i> might play key role in flower color regulation and provide a theoretical reference for blue flower molecular breeding.https://www.mdpi.com/2073-4425/12/7/1086<i>B. acuminata</i>F3′5′Hgene cloningexpression analysis |
| spellingShingle | Min Li Yuting Cao Biswojit Debnath Hongjuan Yang Xiaohua Kui Dongliang Qiu Cloning and Expression Analysis of Flavonoid 3′, 5′-Hydroxylase Gene from <i>Brunfelsia acuminata</i> Genes <i>B. acuminata</i> F3′5′H gene cloning expression analysis |
| title | Cloning and Expression Analysis of Flavonoid 3′, 5′-Hydroxylase Gene from <i>Brunfelsia acuminata</i> |
| title_full | Cloning and Expression Analysis of Flavonoid 3′, 5′-Hydroxylase Gene from <i>Brunfelsia acuminata</i> |
| title_fullStr | Cloning and Expression Analysis of Flavonoid 3′, 5′-Hydroxylase Gene from <i>Brunfelsia acuminata</i> |
| title_full_unstemmed | Cloning and Expression Analysis of Flavonoid 3′, 5′-Hydroxylase Gene from <i>Brunfelsia acuminata</i> |
| title_short | Cloning and Expression Analysis of Flavonoid 3′, 5′-Hydroxylase Gene from <i>Brunfelsia acuminata</i> |
| title_sort | cloning and expression analysis of flavonoid 3 5 hydroxylase gene from i brunfelsia acuminata i |
| topic | <i>B. acuminata</i> F3′5′H gene cloning expression analysis |
| url | https://www.mdpi.com/2073-4425/12/7/1086 |
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