Transcriptome and Proteome Association Analysis to Screen Candidate Genes Related to Salt Tolerance in <i>Reaumuria soongorica</i> Leaves under Salt Stress
This work aims at studying the molecular mechanisms underlying the response of <i>Reaumuria soongorica</i> to salt stress. We used RNA sequencing (RNA-Seq) and Tandem Mass Tag (TMT) techniques to identify differentially expressed genes (DEGs) and differentially expressed proteins (DEPs)...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/20/3542 |
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| author | Hanghang Liu Peifang Chong Shipeng Yan Zehua Liu Xinguang Bao Bingbing Tan |
| author_facet | Hanghang Liu Peifang Chong Shipeng Yan Zehua Liu Xinguang Bao Bingbing Tan |
| author_sort | Hanghang Liu |
| collection | DOAJ |
| description | This work aims at studying the molecular mechanisms underlying the response of <i>Reaumuria soongorica</i> to salt stress. We used RNA sequencing (RNA-Seq) and Tandem Mass Tag (TMT) techniques to identify differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in <i>R. soongorica</i> leaves treated with 0, 200, and 500 mM NaCl for 72 h. The results indicated that compared with the 0 mM NaCl treatment group, 2391 and 6400 DEGs were identified in the 200 and 500 mM NaCl treatment groups, respectively, while 47 and 177 DEPs were also identified. Transcriptome and proteome association analysis was further performed on <i>R. soongorica</i> leaves in the 0/500 mM NaCl treatment group, and 32 genes with consistent mRNA and protein expression trends were identified. <i>SYP71</i>, <i>CS</i>, <i>PCC13-62</i>, <i>PASN</i>, <i>ZIFL1</i>, <i>CHS2</i>, and other differential genes are involved in photosynthesis, vesicle transport, auxin transport, and other functions of plants, and might play a key role in the salt tolerance of <i>R. soongorica</i>. In this study, transcriptome and proteome association techniques were used to screen candidate genes associated with salt tolerance in <i>R. soongorica</i>, which provides an important theoretical basis for understanding the molecular mechanism of salt tolerance in <i>R. soongorica</i> and breeding high-quality germplasm resources. |
| first_indexed | 2024-03-10T20:57:12Z |
| format | Article |
| id | doaj.art-013cb23f4eaa410e8a25d9aa1a6d2d74 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:57:12Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-013cb23f4eaa410e8a25d9aa1a6d2d742023-11-19T17:48:30ZengMDPI AGPlants2223-77472023-10-011220354210.3390/plants12203542Transcriptome and Proteome Association Analysis to Screen Candidate Genes Related to Salt Tolerance in <i>Reaumuria soongorica</i> Leaves under Salt StressHanghang Liu0Peifang Chong1Shipeng Yan2Zehua Liu3Xinguang Bao4Bingbing Tan5College of Forestry, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou 730070, ChinaSchool of Forestry Engineering, Shandong Agriculture and Engineering University, Jinan 250100, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou 730070, ChinaThis work aims at studying the molecular mechanisms underlying the response of <i>Reaumuria soongorica</i> to salt stress. We used RNA sequencing (RNA-Seq) and Tandem Mass Tag (TMT) techniques to identify differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in <i>R. soongorica</i> leaves treated with 0, 200, and 500 mM NaCl for 72 h. The results indicated that compared with the 0 mM NaCl treatment group, 2391 and 6400 DEGs were identified in the 200 and 500 mM NaCl treatment groups, respectively, while 47 and 177 DEPs were also identified. Transcriptome and proteome association analysis was further performed on <i>R. soongorica</i> leaves in the 0/500 mM NaCl treatment group, and 32 genes with consistent mRNA and protein expression trends were identified. <i>SYP71</i>, <i>CS</i>, <i>PCC13-62</i>, <i>PASN</i>, <i>ZIFL1</i>, <i>CHS2</i>, and other differential genes are involved in photosynthesis, vesicle transport, auxin transport, and other functions of plants, and might play a key role in the salt tolerance of <i>R. soongorica</i>. In this study, transcriptome and proteome association techniques were used to screen candidate genes associated with salt tolerance in <i>R. soongorica</i>, which provides an important theoretical basis for understanding the molecular mechanism of salt tolerance in <i>R. soongorica</i> and breeding high-quality germplasm resources.https://www.mdpi.com/2223-7747/12/20/3542<i>Reaumuria soongorica</i>salt stresstranscriptomeproteomeassociation analysis |
| spellingShingle | Hanghang Liu Peifang Chong Shipeng Yan Zehua Liu Xinguang Bao Bingbing Tan Transcriptome and Proteome Association Analysis to Screen Candidate Genes Related to Salt Tolerance in <i>Reaumuria soongorica</i> Leaves under Salt Stress Plants <i>Reaumuria soongorica</i> salt stress transcriptome proteome association analysis |
| title | Transcriptome and Proteome Association Analysis to Screen Candidate Genes Related to Salt Tolerance in <i>Reaumuria soongorica</i> Leaves under Salt Stress |
| title_full | Transcriptome and Proteome Association Analysis to Screen Candidate Genes Related to Salt Tolerance in <i>Reaumuria soongorica</i> Leaves under Salt Stress |
| title_fullStr | Transcriptome and Proteome Association Analysis to Screen Candidate Genes Related to Salt Tolerance in <i>Reaumuria soongorica</i> Leaves under Salt Stress |
| title_full_unstemmed | Transcriptome and Proteome Association Analysis to Screen Candidate Genes Related to Salt Tolerance in <i>Reaumuria soongorica</i> Leaves under Salt Stress |
| title_short | Transcriptome and Proteome Association Analysis to Screen Candidate Genes Related to Salt Tolerance in <i>Reaumuria soongorica</i> Leaves under Salt Stress |
| title_sort | transcriptome and proteome association analysis to screen candidate genes related to salt tolerance in i reaumuria soongorica i leaves under salt stress |
| topic | <i>Reaumuria soongorica</i> salt stress transcriptome proteome association analysis |
| url | https://www.mdpi.com/2223-7747/12/20/3542 |
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