Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate
Magnesium pyrophosphate modified tetracalcium phosphate/monetite cement mixtures (MgTTCPM) were prepared by simple mechanical homogenization of compounds in a ball mill. The MgP<sub>2</sub>O<sub>7</sub> was chosen due to the suitable setting properties of the final cements, i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Materials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1944/15/7/2586 |
| _version_ | 1797438564143726592 |
|---|---|
| author | Radoslava Stulajterova Lubomir Medvecky Maria Giretova Tibor Sopcak Lenka Luptakova Radovan Bures Eva Szekiova |
| author_facet | Radoslava Stulajterova Lubomir Medvecky Maria Giretova Tibor Sopcak Lenka Luptakova Radovan Bures Eva Szekiova |
| author_sort | Radoslava Stulajterova |
| collection | DOAJ |
| description | Magnesium pyrophosphate modified tetracalcium phosphate/monetite cement mixtures (MgTTCPM) were prepared by simple mechanical homogenization of compounds in a ball mill. The MgP<sub>2</sub>O<sub>7</sub> was chosen due to the suitable setting properties of the final cements, in contrast to cements with the addition of amorphous (Ca, Mg) CO<sub>3</sub> or newberite, which significantly extended the setting time even in small amounts (corresponding ~to 1 wt% of Mg in final cements). The results showed the gradual dissolution of the same amount of Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> phase, regardless of its content in the cement mixtures, and the refinement of formed HAP nanoparticles, which were joined into weakly and mutually bound spherical agglomerates. The compressive strength of composite cements was reduced to 14 MPa and the setting time was 5–10 min depending on the composition. Cytotoxicity of cements or their extracts was not detected and increased proliferative activity of mesenchymal stem cells with upregulation of osteopontin and osteonectin genes was verified in cells cultured for 7 and 15 days in cement extracts. The above facts, including insignificant changes in the pH of simulated body fluid solution and mechanical strength close to cancellous bone, indicate that MgTTCPM cement mixtures could be suitable biomaterials for use in the treatment of bone defects. |
| first_indexed | 2024-03-09T11:39:47Z |
| format | Article |
| id | doaj.art-013cbd52490b45b5b54f6e2ce136c863 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T11:39:47Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-013cbd52490b45b5b54f6e2ce136c8632023-11-30T23:34:11ZengMDPI AGMaterials1996-19442022-03-01157258610.3390/ma15072586Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium PyrophosphateRadoslava Stulajterova0Lubomir Medvecky1Maria Giretova2Tibor Sopcak3Lenka Luptakova4Radovan Bures5Eva Szekiova6Division of Functional and Hybrid Systems, Institute of Materials Research of SAS, Watsonova 47, 040 01 Kosice, SlovakiaDivision of Functional and Hybrid Systems, Institute of Materials Research of SAS, Watsonova 47, 040 01 Kosice, SlovakiaDivision of Functional and Hybrid Systems, Institute of Materials Research of SAS, Watsonova 47, 040 01 Kosice, SlovakiaDivision of Functional and Hybrid Systems, Institute of Materials Research of SAS, Watsonova 47, 040 01 Kosice, SlovakiaDepartment of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, SlovakiaDivision of Functional and Hybrid Systems, Institute of Materials Research of SAS, Watsonova 47, 040 01 Kosice, SlovakiaInstitute of Neurobiology of Biomedical Research Center of SAS, Soltesovej 4–6, 040 01 Kosice, SlovakiaMagnesium pyrophosphate modified tetracalcium phosphate/monetite cement mixtures (MgTTCPM) were prepared by simple mechanical homogenization of compounds in a ball mill. The MgP<sub>2</sub>O<sub>7</sub> was chosen due to the suitable setting properties of the final cements, in contrast to cements with the addition of amorphous (Ca, Mg) CO<sub>3</sub> or newberite, which significantly extended the setting time even in small amounts (corresponding ~to 1 wt% of Mg in final cements). The results showed the gradual dissolution of the same amount of Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> phase, regardless of its content in the cement mixtures, and the refinement of formed HAP nanoparticles, which were joined into weakly and mutually bound spherical agglomerates. The compressive strength of composite cements was reduced to 14 MPa and the setting time was 5–10 min depending on the composition. Cytotoxicity of cements or their extracts was not detected and increased proliferative activity of mesenchymal stem cells with upregulation of osteopontin and osteonectin genes was verified in cells cultured for 7 and 15 days in cement extracts. The above facts, including insignificant changes in the pH of simulated body fluid solution and mechanical strength close to cancellous bone, indicate that MgTTCPM cement mixtures could be suitable biomaterials for use in the treatment of bone defects.https://www.mdpi.com/1996-1944/15/7/2586calcium phosphate biocementmagnesium pyrophosphatesetting processmesenchymal stem cellsgene expression |
| spellingShingle | Radoslava Stulajterova Lubomir Medvecky Maria Giretova Tibor Sopcak Lenka Luptakova Radovan Bures Eva Szekiova Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate Materials calcium phosphate biocement magnesium pyrophosphate setting process mesenchymal stem cells gene expression |
| title | Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate |
| title_full | Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate |
| title_fullStr | Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate |
| title_full_unstemmed | Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate |
| title_short | Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate |
| title_sort | characterization of tetracalcium phosphate monetite biocement modified by magnesium pyrophosphate |
| topic | calcium phosphate biocement magnesium pyrophosphate setting process mesenchymal stem cells gene expression |
| url | https://www.mdpi.com/1996-1944/15/7/2586 |
| work_keys_str_mv | AT radoslavastulajterova characterizationoftetracalciumphosphatemonetitebiocementmodifiedbymagnesiumpyrophosphate AT lubomirmedvecky characterizationoftetracalciumphosphatemonetitebiocementmodifiedbymagnesiumpyrophosphate AT mariagiretova characterizationoftetracalciumphosphatemonetitebiocementmodifiedbymagnesiumpyrophosphate AT tiborsopcak characterizationoftetracalciumphosphatemonetitebiocementmodifiedbymagnesiumpyrophosphate AT lenkaluptakova characterizationoftetracalciumphosphatemonetitebiocementmodifiedbymagnesiumpyrophosphate AT radovanbures characterizationoftetracalciumphosphatemonetitebiocementmodifiedbymagnesiumpyrophosphate AT evaszekiova characterizationoftetracalciumphosphatemonetitebiocementmodifiedbymagnesiumpyrophosphate |