Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathways
Glioblastoma (GBM) is a very aggressive tumor that presents vascularization, necrosis and is resistant to chemotherapy and radiotherapy. Current treatments are not effective eradicating GBM, thus, there is an urgent need to develop novel therapeutic strategies against GBM. AZD5363, AZD8542, curcumin...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
|
| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580823000110 |
| _version_ | 1811167214846345216 |
|---|---|
| author | Rosalinda Mejía-Rodríguez Daniel Romero-Trejo Rosa O. González José Segovia |
| author_facet | Rosalinda Mejía-Rodríguez Daniel Romero-Trejo Rosa O. González José Segovia |
| author_sort | Rosalinda Mejía-Rodríguez |
| collection | DOAJ |
| description | Glioblastoma (GBM) is a very aggressive tumor that presents vascularization, necrosis and is resistant to chemotherapy and radiotherapy. Current treatments are not effective eradicating GBM, thus, there is an urgent need to develop novel therapeutic strategies against GBM. AZD5363, AZD8542, curcumin and resveratrol, are widely studied for the treatment of cancer and in the present study we explored the effects of the administration of combined treatments with AZD5363, AZD8542, curcumin or resveratrol on human GBM cells. We found that the combined treatments with AZD5363+AZD8542+Curcumin and AZD8542+Curcumin+Resveratrol inhibit the PI3K/AKT and SHH survival pathways by decreasing the activity of AKT, the reduction of the expression of SMO, pP70S6k, pS6k, GLI1, p21 and p27, and the activation of caspase-3 as a marker of apoptosis. These results provide evidence that the combined treatments AZD5363+AZD8542+Curcumin and AZD8542+Curcumin+Resveratrol have the potential to be an interesting option against GBM. |
| first_indexed | 2024-04-10T16:06:37Z |
| format | Article |
| id | doaj.art-013f5645e1b44dfd91eab41e270c3fc6 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-04-10T16:06:37Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-013f5645e1b44dfd91eab41e270c3fc62023-02-10T04:22:50ZengElsevierBiochemistry and Biophysics Reports2405-58082023-03-0133101430Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathwaysRosalinda Mejía-Rodríguez0Daniel Romero-Trejo1Rosa O. González2José Segovia3Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, MexicoDepartamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, MexicoDepartamento de Matemáticas, Universidad Autónoma Metropolitana-Iztapalapa (UAM-I), MexicoDepartamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, Mexico; Corresponding author. Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN # 2508, 07300, Mexico.Glioblastoma (GBM) is a very aggressive tumor that presents vascularization, necrosis and is resistant to chemotherapy and radiotherapy. Current treatments are not effective eradicating GBM, thus, there is an urgent need to develop novel therapeutic strategies against GBM. AZD5363, AZD8542, curcumin and resveratrol, are widely studied for the treatment of cancer and in the present study we explored the effects of the administration of combined treatments with AZD5363, AZD8542, curcumin or resveratrol on human GBM cells. We found that the combined treatments with AZD5363+AZD8542+Curcumin and AZD8542+Curcumin+Resveratrol inhibit the PI3K/AKT and SHH survival pathways by decreasing the activity of AKT, the reduction of the expression of SMO, pP70S6k, pS6k, GLI1, p21 and p27, and the activation of caspase-3 as a marker of apoptosis. These results provide evidence that the combined treatments AZD5363+AZD8542+Curcumin and AZD8542+Curcumin+Resveratrol have the potential to be an interesting option against GBM.http://www.sciencedirect.com/science/article/pii/S2405580823000110GliomaCombined therapyResveratrolCurcuminAZD5363AZD8542 |
| spellingShingle | Rosalinda Mejía-Rodríguez Daniel Romero-Trejo Rosa O. González José Segovia Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathways Biochemistry and Biophysics Reports Glioma Combined therapy Resveratrol Curcumin AZD5363 AZD8542 |
| title | Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathways |
| title_full | Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathways |
| title_fullStr | Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathways |
| title_full_unstemmed | Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathways |
| title_short | Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathways |
| title_sort | combined treatments with azd5363 azd8542 curcumin or resveratrol induce death of human glioblastoma cells by suppressing the pi3k akt and shh signaling pathways |
| topic | Glioma Combined therapy Resveratrol Curcumin AZD5363 AZD8542 |
| url | http://www.sciencedirect.com/science/article/pii/S2405580823000110 |
| work_keys_str_mv | AT rosalindamejiarodriguez combinedtreatmentswithazd5363azd8542curcuminorresveratrolinducedeathofhumanglioblastomacellsbysuppressingthepi3kaktandshhsignalingpathways AT danielromerotrejo combinedtreatmentswithazd5363azd8542curcuminorresveratrolinducedeathofhumanglioblastomacellsbysuppressingthepi3kaktandshhsignalingpathways AT rosaogonzalez combinedtreatmentswithazd5363azd8542curcuminorresveratrolinducedeathofhumanglioblastomacellsbysuppressingthepi3kaktandshhsignalingpathways AT josesegovia combinedtreatmentswithazd5363azd8542curcuminorresveratrolinducedeathofhumanglioblastomacellsbysuppressingthepi3kaktandshhsignalingpathways |