LED Zappelin’: An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging
Two-photon (2P) microscopy is a cornerstone technique in neuroscience research. However, combining 2P imaging with spectrally arbitrary light stimulation can be challenging due to crosstalk between stimulation light and fluorescence detection. To overcome this limitation, we present a simple and low...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | HardwareX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468067220300365 |
| _version_ | 1818622057181609984 |
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| author | M.J.Y. Zimmermann A. Maia Chagas P. Bartel S. Pop L.L. Prieto-Godino T. Baden |
| author_facet | M.J.Y. Zimmermann A. Maia Chagas P. Bartel S. Pop L.L. Prieto-Godino T. Baden |
| author_sort | M.J.Y. Zimmermann |
| collection | DOAJ |
| description | Two-photon (2P) microscopy is a cornerstone technique in neuroscience research. However, combining 2P imaging with spectrally arbitrary light stimulation can be challenging due to crosstalk between stimulation light and fluorescence detection. To overcome this limitation, we present a simple and low-cost electronic solution based on an ESP32 microcontroller and a TLC5947 LED driver to rapidly time-interleave stimulation and detection epochs during scans. Implemented for less than $100, our design can independently drive up to 24 arbitrary spectrum LEDs to meet user requirements. We demonstrate the utility of our stimulator for colour vision experiments on the in vivo tetrachromatic zebrafish retina and for optogenetic circuit mapping in Drosophila. |
| first_indexed | 2024-12-16T18:19:07Z |
| format | Article |
| id | doaj.art-014007a4dd774f15a95d731751242980 |
| institution | Directory Open Access Journal |
| issn | 2468-0672 |
| language | English |
| last_indexed | 2024-12-16T18:19:07Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | HardwareX |
| spelling | doaj.art-014007a4dd774f15a95d7317512429802022-12-21T22:21:36ZengElsevierHardwareX2468-06722020-10-018e00127LED Zappelin’: An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imagingM.J.Y. Zimmermann0A. Maia Chagas1P. Bartel2S. Pop3L.L. Prieto-Godino4T. Baden5Sussex Neuroscience, School of Life Sciences, University of Sussex, United Kingdom; Corresponding authors at: Sussex Neuroscience, School of Life Sciences, University of Sussex, United Kingdom (M.J.Y. Zimmermann).Sussex Neuroscience, School of Life Sciences, University of Sussex, United Kingdom; TReND in Africa (www.TReNDinAfrica.org), United Kingdom; GOSH Community (http://openhardware.science/); Institute for Ophthalmic Research, University of Tübingen, GermanySussex Neuroscience, School of Life Sciences, University of Sussex, United KingdomThe Francis Crick Institute, London, United KingdomTReND in Africa (www.TReNDinAfrica.org), United Kingdom; The Francis Crick Institute, London, United KingdomSussex Neuroscience, School of Life Sciences, University of Sussex, United Kingdom; TReND in Africa (www.TReNDinAfrica.org), United Kingdom; Institute for Ophthalmic Research, University of Tübingen, Germany; Corresponding authors at: Sussex Neuroscience, School of Life Sciences, University of Sussex, United Kingdom (M.J.Y. Zimmermann).Two-photon (2P) microscopy is a cornerstone technique in neuroscience research. However, combining 2P imaging with spectrally arbitrary light stimulation can be challenging due to crosstalk between stimulation light and fluorescence detection. To overcome this limitation, we present a simple and low-cost electronic solution based on an ESP32 microcontroller and a TLC5947 LED driver to rapidly time-interleave stimulation and detection epochs during scans. Implemented for less than $100, our design can independently drive up to 24 arbitrary spectrum LEDs to meet user requirements. We demonstrate the utility of our stimulator for colour vision experiments on the in vivo tetrachromatic zebrafish retina and for optogenetic circuit mapping in Drosophila.http://www.sciencedirect.com/science/article/pii/S24680672203003652 PhotonVisual neuroscienceOptogeneticsLight stimulationLEDOpen hardware |
| spellingShingle | M.J.Y. Zimmermann A. Maia Chagas P. Bartel S. Pop L.L. Prieto-Godino T. Baden LED Zappelin’: An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging HardwareX 2 Photon Visual neuroscience Optogenetics Light stimulation LED Open hardware |
| title | LED Zappelin’: An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging |
| title_full | LED Zappelin’: An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging |
| title_fullStr | LED Zappelin’: An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging |
| title_full_unstemmed | LED Zappelin’: An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging |
| title_short | LED Zappelin’: An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging |
| title_sort | led zappelin an open source led controller for arbitrary spectrum visual stimulation and optogenetics during 2 photon imaging |
| topic | 2 Photon Visual neuroscience Optogenetics Light stimulation LED Open hardware |
| url | http://www.sciencedirect.com/science/article/pii/S2468067220300365 |
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