Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.
Aging in organisms as diverse as yeast, nematodes, and mammals is delayed by caloric restriction, an effect mediated by the nutrient sensing TOR, RAS/cAMP, and AKT/Sch9 pathways. The transcription factor Gis1 functions downstream of these pathways in extending the lifespan of nutrient restricted yea...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363679/pdf/?tool=EBI |
| _version_ | 1818570389707554816 |
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| author | Jakub Orzechowski Westholm Susanna Tronnersjö Niklas Nordberg Ida Olsson Jan Komorowski Hans Ronne |
| author_facet | Jakub Orzechowski Westholm Susanna Tronnersjö Niklas Nordberg Ida Olsson Jan Komorowski Hans Ronne |
| author_sort | Jakub Orzechowski Westholm |
| collection | DOAJ |
| description | Aging in organisms as diverse as yeast, nematodes, and mammals is delayed by caloric restriction, an effect mediated by the nutrient sensing TOR, RAS/cAMP, and AKT/Sch9 pathways. The transcription factor Gis1 functions downstream of these pathways in extending the lifespan of nutrient restricted yeast cells, but the mechanisms involved are still poorly understood. We have used gene expression microarrays to study the targets of Gis1 and the related protein Rph1 in different growth phases. Our results show that Gis1 and Rph1 act both as repressors and activators, on overlapping sets of genes as well as on distinct targets. Interestingly, both the activities and the target specificities of Gis1 and Rph1 depend on the growth phase. Thus, both proteins are associated with repression during exponential growth, targeting genes with STRE or PDS motifs in their promoters. After the diauxic shift, both become involved in activation, with Gis1 acting primarily on genes with PDS motifs, and Rph1 on genes with STRE motifs. Significantly, Gis1 and Rph1 control a number of genes involved in acetate and glycerol formation, metabolites that have been implicated in aging. Furthermore, several genes involved in acetyl-CoA metabolism are downregulated by Gis1. |
| first_indexed | 2024-12-14T13:40:42Z |
| format | Article |
| id | doaj.art-01445a9169cc42c5a7c83deb59272869 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T13:40:42Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-01445a9169cc42c5a7c83deb592728692022-12-21T22:59:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3157710.1371/journal.pone.0031577Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.Jakub Orzechowski WestholmSusanna TronnersjöNiklas NordbergIda OlssonJan KomorowskiHans RonneAging in organisms as diverse as yeast, nematodes, and mammals is delayed by caloric restriction, an effect mediated by the nutrient sensing TOR, RAS/cAMP, and AKT/Sch9 pathways. The transcription factor Gis1 functions downstream of these pathways in extending the lifespan of nutrient restricted yeast cells, but the mechanisms involved are still poorly understood. We have used gene expression microarrays to study the targets of Gis1 and the related protein Rph1 in different growth phases. Our results show that Gis1 and Rph1 act both as repressors and activators, on overlapping sets of genes as well as on distinct targets. Interestingly, both the activities and the target specificities of Gis1 and Rph1 depend on the growth phase. Thus, both proteins are associated with repression during exponential growth, targeting genes with STRE or PDS motifs in their promoters. After the diauxic shift, both become involved in activation, with Gis1 acting primarily on genes with PDS motifs, and Rph1 on genes with STRE motifs. Significantly, Gis1 and Rph1 control a number of genes involved in acetate and glycerol formation, metabolites that have been implicated in aging. Furthermore, several genes involved in acetyl-CoA metabolism are downregulated by Gis1.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363679/pdf/?tool=EBI |
| spellingShingle | Jakub Orzechowski Westholm Susanna Tronnersjö Niklas Nordberg Ida Olsson Jan Komorowski Hans Ronne Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells. PLoS ONE |
| title | Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells. |
| title_full | Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells. |
| title_fullStr | Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells. |
| title_full_unstemmed | Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells. |
| title_short | Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells. |
| title_sort | gis1 and rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363679/pdf/?tool=EBI |
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