The Mechanism of Dynamic Interaction between Doxorubicin and Calf Thymus DNA at the Single-Molecule Level Based on Confocal Raman Spectroscopy
It is of great fundamental significance and practical application to understand the binding sites and dynamic process of the interaction between doxorubicin (DOX) and DNA molecules. Based on the Confocal Raman spectroscopy, the interaction between DOX and calf thymus DNA has been systemically invest...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/6/940 |
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| author | Ruihong Zhang Jie Zhu Dan Sun Jie Li Lina Yao Shuangshuang Meng Yan Li Yang Dang Kaige Wang |
| author_facet | Ruihong Zhang Jie Zhu Dan Sun Jie Li Lina Yao Shuangshuang Meng Yan Li Yang Dang Kaige Wang |
| author_sort | Ruihong Zhang |
| collection | DOAJ |
| description | It is of great fundamental significance and practical application to understand the binding sites and dynamic process of the interaction between doxorubicin (DOX) and DNA molecules. Based on the Confocal Raman spectroscopy, the interaction between DOX and calf thymus DNA has been systemically investigated, and some meaningful findings have been found. DOX molecules can not only interact with all four bases of DNA molecules, i.e., adenine, thymine, cytosine, guanine, and phosphate, but also affect the DNA conformation. Meanwhile, the binding site of DOX and its derivatives such as daunorubicin and epirubicin is certain. Furthermore, the interaction between DOX and DNA molecules is a dynamic process since the intensities of each characteristic peaks of the base, e.g., adenine, cytosine, and phosphate, are all regularly changed with the interaction time. Finally, a dynamic mechanism model of the interaction between DOX and DNA molecules is proposed; that is, there are two kinds of interaction between DOX and DNA molecules: DOX-DNA acts to form a complex, and DOX-DOX acts to form a multimer. The two effects are competitive, as the former compresses DNA molecules, and the latter decompresses these DNA molecules. This work is helpful for accurately understanding and developing new drugs and pathways to improve and treat DOX-induced cytotoxicity and cardiotoxicity. |
| first_indexed | 2024-03-09T23:00:38Z |
| format | Article |
| id | doaj.art-01447a5effd240218d227931e497429e |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T23:00:38Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-01447a5effd240218d227931e497429e2023-11-23T18:01:56ZengMDPI AGMicromachines2072-666X2022-06-0113694010.3390/mi13060940The Mechanism of Dynamic Interaction between Doxorubicin and Calf Thymus DNA at the Single-Molecule Level Based on Confocal Raman SpectroscopyRuihong Zhang0Jie Zhu1Dan Sun2Jie Li3Lina Yao4Shuangshuang Meng5Yan Li6Yang Dang7Kaige Wang8State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials; National Center for International Research of Photoelectric Technology & Nano-Functional Materials and Application; Shaanxi Provincial Key Laboratory of Photoelectric Technology; Institute of Photonics and Photon-Technology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials; National Center for International Research of Photoelectric Technology & Nano-Functional Materials and Application; Shaanxi Provincial Key Laboratory of Photoelectric Technology; Institute of Photonics and Photon-Technology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials; National Center for International Research of Photoelectric Technology & Nano-Functional Materials and Application; Shaanxi Provincial Key Laboratory of Photoelectric Technology; Institute of Photonics and Photon-Technology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials; National Center for International Research of Photoelectric Technology & Nano-Functional Materials and Application; Shaanxi Provincial Key Laboratory of Photoelectric Technology; Institute of Photonics and Photon-Technology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials; National Center for International Research of Photoelectric Technology & Nano-Functional Materials and Application; Shaanxi Provincial Key Laboratory of Photoelectric Technology; Institute of Photonics and Photon-Technology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials; National Center for International Research of Photoelectric Technology & Nano-Functional Materials and Application; Shaanxi Provincial Key Laboratory of Photoelectric Technology; Institute of Photonics and Photon-Technology, Northwest University, Xi’an 710069, ChinaSchool of Science, Xi’an Shiyou University, Xi’an 710069, ChinaState Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials; National Center for International Research of Photoelectric Technology & Nano-Functional Materials and Application; Shaanxi Provincial Key Laboratory of Photoelectric Technology; Institute of Photonics and Photon-Technology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials; National Center for International Research of Photoelectric Technology & Nano-Functional Materials and Application; Shaanxi Provincial Key Laboratory of Photoelectric Technology; Institute of Photonics and Photon-Technology, Northwest University, Xi’an 710069, ChinaIt is of great fundamental significance and practical application to understand the binding sites and dynamic process of the interaction between doxorubicin (DOX) and DNA molecules. Based on the Confocal Raman spectroscopy, the interaction between DOX and calf thymus DNA has been systemically investigated, and some meaningful findings have been found. DOX molecules can not only interact with all four bases of DNA molecules, i.e., adenine, thymine, cytosine, guanine, and phosphate, but also affect the DNA conformation. Meanwhile, the binding site of DOX and its derivatives such as daunorubicin and epirubicin is certain. Furthermore, the interaction between DOX and DNA molecules is a dynamic process since the intensities of each characteristic peaks of the base, e.g., adenine, cytosine, and phosphate, are all regularly changed with the interaction time. Finally, a dynamic mechanism model of the interaction between DOX and DNA molecules is proposed; that is, there are two kinds of interaction between DOX and DNA molecules: DOX-DNA acts to form a complex, and DOX-DOX acts to form a multimer. The two effects are competitive, as the former compresses DNA molecules, and the latter decompresses these DNA molecules. This work is helpful for accurately understanding and developing new drugs and pathways to improve and treat DOX-induced cytotoxicity and cardiotoxicity.https://www.mdpi.com/2072-666X/13/6/940doxorubicincalf thymus DNAbinding sitedynamic processConfocal Raman spectroscopy |
| spellingShingle | Ruihong Zhang Jie Zhu Dan Sun Jie Li Lina Yao Shuangshuang Meng Yan Li Yang Dang Kaige Wang The Mechanism of Dynamic Interaction between Doxorubicin and Calf Thymus DNA at the Single-Molecule Level Based on Confocal Raman Spectroscopy Micromachines doxorubicin calf thymus DNA binding site dynamic process Confocal Raman spectroscopy |
| title | The Mechanism of Dynamic Interaction between Doxorubicin and Calf Thymus DNA at the Single-Molecule Level Based on Confocal Raman Spectroscopy |
| title_full | The Mechanism of Dynamic Interaction between Doxorubicin and Calf Thymus DNA at the Single-Molecule Level Based on Confocal Raman Spectroscopy |
| title_fullStr | The Mechanism of Dynamic Interaction between Doxorubicin and Calf Thymus DNA at the Single-Molecule Level Based on Confocal Raman Spectroscopy |
| title_full_unstemmed | The Mechanism of Dynamic Interaction between Doxorubicin and Calf Thymus DNA at the Single-Molecule Level Based on Confocal Raman Spectroscopy |
| title_short | The Mechanism of Dynamic Interaction between Doxorubicin and Calf Thymus DNA at the Single-Molecule Level Based on Confocal Raman Spectroscopy |
| title_sort | mechanism of dynamic interaction between doxorubicin and calf thymus dna at the single molecule level based on confocal raman spectroscopy |
| topic | doxorubicin calf thymus DNA binding site dynamic process Confocal Raman spectroscopy |
| url | https://www.mdpi.com/2072-666X/13/6/940 |
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