Identification of a C<sub>2</sub>H<sub>2</sub> Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in <i>Phytophthora sojae</i> Based on WGCNA
<i>Phytophthora sojae</i> is a destructive soybean pathogen that orchestrates various secreted proteins (effectors) to modulate plant immunity and facilitate infection. Although a number of effectors have been identified and functionally studied in <i>P. sojae</i>, the way th...
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MDPI AG
2022-09-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/10/998 |
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| author | Yanhong Hu Zhihua He Yebin Kang Wenwu Ye Linkai Cui |
| author_facet | Yanhong Hu Zhihua He Yebin Kang Wenwu Ye Linkai Cui |
| author_sort | Yanhong Hu |
| collection | DOAJ |
| description | <i>Phytophthora sojae</i> is a destructive soybean pathogen that orchestrates various secreted proteins (effectors) to modulate plant immunity and facilitate infection. Although a number of effectors have been identified and functionally studied in <i>P. sojae</i>, the way these molecules are regulated is marginally known. In this study, we performed a weighted gene correlation network analysis (WGCNA) based on digital RNA-seq, which enabled the identification of a transcription factor (PsCZF3) in <i>P. sojae</i>. This transcription factor is a C<sub>2</sub>H<sub>2</sub>-type zinc finger protein that regulates the transcription of 35 RxLR effectors during the early infection stage. Phylogenetic analysis revealed that PsCZF3 is a highly conserved protein across oomycetes, suggesting that this regulation mechanism may broadly exist in oomycete species. In addition, by building a subnetwork of <i>PsCZF3</i> and correlated genes, we also found that PsCZF3 contributed to the transcriptional regulation of carbohydrate-active enzymes. Our findings suggest that the activation of <i>PsCZF3</i> facilitates <i>P. sojae</i> infection by up-regulating RxLR effectors and carbohydrate-active enzymes. |
| first_indexed | 2024-03-09T19:59:34Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T19:59:34Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-0144a1b6f6794fb991f6f8b0701bf6242023-11-24T00:46:26ZengMDPI AGJournal of Fungi2309-608X2022-09-0181099810.3390/jof8100998Identification of a C<sub>2</sub>H<sub>2</sub> Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in <i>Phytophthora sojae</i> Based on WGCNAYanhong Hu0Zhihua He1Yebin Kang2Wenwu Ye3Linkai Cui4College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471000, ChinaDepartment of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471000, China<i>Phytophthora sojae</i> is a destructive soybean pathogen that orchestrates various secreted proteins (effectors) to modulate plant immunity and facilitate infection. Although a number of effectors have been identified and functionally studied in <i>P. sojae</i>, the way these molecules are regulated is marginally known. In this study, we performed a weighted gene correlation network analysis (WGCNA) based on digital RNA-seq, which enabled the identification of a transcription factor (PsCZF3) in <i>P. sojae</i>. This transcription factor is a C<sub>2</sub>H<sub>2</sub>-type zinc finger protein that regulates the transcription of 35 RxLR effectors during the early infection stage. Phylogenetic analysis revealed that PsCZF3 is a highly conserved protein across oomycetes, suggesting that this regulation mechanism may broadly exist in oomycete species. In addition, by building a subnetwork of <i>PsCZF3</i> and correlated genes, we also found that PsCZF3 contributed to the transcriptional regulation of carbohydrate-active enzymes. Our findings suggest that the activation of <i>PsCZF3</i> facilitates <i>P. sojae</i> infection by up-regulating RxLR effectors and carbohydrate-active enzymes.https://www.mdpi.com/2309-608X/8/10/998digital RNA-seq<i>Phytophthora sojae</i>WGCNAtranscription factorRxLR effector |
| spellingShingle | Yanhong Hu Zhihua He Yebin Kang Wenwu Ye Linkai Cui Identification of a C<sub>2</sub>H<sub>2</sub> Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in <i>Phytophthora sojae</i> Based on WGCNA Journal of Fungi digital RNA-seq <i>Phytophthora sojae</i> WGCNA transcription factor RxLR effector |
| title | Identification of a C<sub>2</sub>H<sub>2</sub> Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in <i>Phytophthora sojae</i> Based on WGCNA |
| title_full | Identification of a C<sub>2</sub>H<sub>2</sub> Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in <i>Phytophthora sojae</i> Based on WGCNA |
| title_fullStr | Identification of a C<sub>2</sub>H<sub>2</sub> Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in <i>Phytophthora sojae</i> Based on WGCNA |
| title_full_unstemmed | Identification of a C<sub>2</sub>H<sub>2</sub> Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in <i>Phytophthora sojae</i> Based on WGCNA |
| title_short | Identification of a C<sub>2</sub>H<sub>2</sub> Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in <i>Phytophthora sojae</i> Based on WGCNA |
| title_sort | identification of a c sub 2 sub h sub 2 sub transcription factor psczf3 associated with rxlr effectors and carbohydrate active enzymes in i phytophthora sojae i based on wgcna |
| topic | digital RNA-seq <i>Phytophthora sojae</i> WGCNA transcription factor RxLR effector |
| url | https://www.mdpi.com/2309-608X/8/10/998 |
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