Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves
Abstract Cryptosporidiosis is one of the main causes of diarrhea in children and young livestock. The interaction of the parasite with the intestinal host cells has not been characterized thoroughly yet but may be affected by the nutritional demand of the parasite. Hence, we aimed to investigate the...
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| Format: | Article |
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BMC
2023-05-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-023-01172-y |
| _version_ | 1797822711329718272 |
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| author | Franziska Dengler Harald M. Hammon Wendy Liermann Solvig Görs Lisa Bachmann Christiane Helm Reiner Ulrich Cora Delling |
| author_facet | Franziska Dengler Harald M. Hammon Wendy Liermann Solvig Görs Lisa Bachmann Christiane Helm Reiner Ulrich Cora Delling |
| author_sort | Franziska Dengler |
| collection | DOAJ |
| description | Abstract Cryptosporidiosis is one of the main causes of diarrhea in children and young livestock. The interaction of the parasite with the intestinal host cells has not been characterized thoroughly yet but may be affected by the nutritional demand of the parasite. Hence, we aimed to investigate the impact of C. parvum infection on glucose metabolism in neonatal calves. Therefore, N = 5 neonatal calves were infected with C. parvum on the first day of life, whereas a control group was not (N = 5). The calves were monitored clinically for one week, and glucose absorption, turnover and oxidation were assessed using stable isotope labelled glucose. The transepithelial transport of glucose was measured using the Ussing chamber technique. Glucose transporters were quantified on gene and protein expression level using RT-qPCR and Western blot in the jejunum epithelium and brush border membrane preparations. Plasma glucose concentration and oral glucose absorption were decreased despite an increased electrogenic phlorizin sensitive transepithelial transport of glucose in infected calves. No difference in the gene or protein abundance of glucose transporters, but an enrichment of glucose transporter 2 in the brush border was observed in the infected calves. Furthermore, the mRNA for enzymes of the glycolysis pathway was increased indicating enhanced glucose oxidation in the infected gut. In summary, C. parvum infection modulates intestinal epithelial glucose absorption and metabolism. We assume that the metabolic competition of the parasite for glucose causes the host cells to upregulate their uptake mechanisms and metabolic machinery to compensate for the energy losses. |
| first_indexed | 2024-03-13T10:12:10Z |
| format | Article |
| id | doaj.art-0145e73f249a4128a001f8800101f04c |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-03-13T10:12:10Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-0145e73f249a4128a001f8800101f04c2023-05-21T11:23:36ZengBMCVeterinary Research1297-97162023-05-0154111610.1186/s13567-023-01172-yCryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calvesFranziska Dengler0Harald M. Hammon1Wendy Liermann2Solvig Görs3Lisa Bachmann4Christiane Helm5Reiner Ulrich6Cora Delling7Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine ViennaResearch Institute for Farm Animal Biology (FBN), Institute of Nutritional PhysiologyResearch Institute for Farm Animal Biology (FBN), Institute of Nutritional PhysiologyResearch Institute for Farm Animal Biology (FBN), Institute of Nutritional PhysiologyResearch Institute for Farm Animal Biology (FBN), Institute of Nutritional PhysiologyInstitute of Veterinary Pathology, Leipzig UniversityInstitute of Veterinary Pathology, Leipzig UniversityInstitute of Parasitology, Leipzig UniversityAbstract Cryptosporidiosis is one of the main causes of diarrhea in children and young livestock. The interaction of the parasite with the intestinal host cells has not been characterized thoroughly yet but may be affected by the nutritional demand of the parasite. Hence, we aimed to investigate the impact of C. parvum infection on glucose metabolism in neonatal calves. Therefore, N = 5 neonatal calves were infected with C. parvum on the first day of life, whereas a control group was not (N = 5). The calves were monitored clinically for one week, and glucose absorption, turnover and oxidation were assessed using stable isotope labelled glucose. The transepithelial transport of glucose was measured using the Ussing chamber technique. Glucose transporters were quantified on gene and protein expression level using RT-qPCR and Western blot in the jejunum epithelium and brush border membrane preparations. Plasma glucose concentration and oral glucose absorption were decreased despite an increased electrogenic phlorizin sensitive transepithelial transport of glucose in infected calves. No difference in the gene or protein abundance of glucose transporters, but an enrichment of glucose transporter 2 in the brush border was observed in the infected calves. Furthermore, the mRNA for enzymes of the glycolysis pathway was increased indicating enhanced glucose oxidation in the infected gut. In summary, C. parvum infection modulates intestinal epithelial glucose absorption and metabolism. We assume that the metabolic competition of the parasite for glucose causes the host cells to upregulate their uptake mechanisms and metabolic machinery to compensate for the energy losses.https://doi.org/10.1186/s13567-023-01172-yCryptosporidiosisGLUT2in vivometabolismSGLT1Ussing chamber |
| spellingShingle | Franziska Dengler Harald M. Hammon Wendy Liermann Solvig Görs Lisa Bachmann Christiane Helm Reiner Ulrich Cora Delling Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves Veterinary Research Cryptosporidiosis GLUT2 in vivo metabolism SGLT1 Ussing chamber |
| title | Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves |
| title_full | Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves |
| title_fullStr | Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves |
| title_full_unstemmed | Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves |
| title_short | Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves |
| title_sort | cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves |
| topic | Cryptosporidiosis GLUT2 in vivo metabolism SGLT1 Ussing chamber |
| url | https://doi.org/10.1186/s13567-023-01172-y |
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