Increased root hair density by loss of WRKY6 in Arabidopsis thaliana
Root hairs are unicellular elongations of certain rhizodermal cells that improve the uptake of sparingly soluble and immobile soil nutrients. Among different Arabidopsis thaliana genotypes, root hair density, length and the local acclimation to low inorganic phosphate (Pi) differs considerably, when...
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PeerJ Inc.
2017-01-01
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| Online Access: | https://peerj.com/articles/2891.pdf |
| _version_ | 1797419474083643392 |
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| author | Markus G. Stetter Martin Benz Uwe Ludewig |
| author_facet | Markus G. Stetter Martin Benz Uwe Ludewig |
| author_sort | Markus G. Stetter |
| collection | DOAJ |
| description | Root hairs are unicellular elongations of certain rhizodermal cells that improve the uptake of sparingly soluble and immobile soil nutrients. Among different Arabidopsis thaliana genotypes, root hair density, length and the local acclimation to low inorganic phosphate (Pi) differs considerably, when analyzed on split agar plates. Here, genome-wide association fine mapping identified significant single nucleotide polymorphisms associated with the increased root hair density in the absence of local phosphate on chromosome 1. A loss-of-functionmutant of the candidate transcription factor gene WRKY6, which is involved in the acclimation of plants to low phosphorus, had increased root hair density. This is partially explained by a reduced cortical cell diameter in wrky6-3, reducing the rhizodermal cell numbers adjacent to the cortical cells. As a consequence, rhizodermal cells in positions that are in contact with two cortical cells are found more often, leading to higher hair density. Distinct cortical cell diameters and epidermal cell lengths distinguish other Arabidopsis accessions with distinct root hair density and −Pi response from diploid Col-0, while tetraploid Col-0 had generally larger root cell sizes, which explain longer hairs. A distinct radial root morphology within Arabidopsis accessions and wrky6-3explains some, but not all, differences in the root hair acclimation to –Pi. |
| first_indexed | 2024-03-09T06:47:51Z |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:47:51Z |
| publishDate | 2017-01-01 |
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| spelling | doaj.art-0146b7de056243d192d5c60e8d88283c2023-12-03T10:31:49ZengPeerJ Inc.PeerJ2167-83592017-01-015e289110.7717/peerj.2891Increased root hair density by loss of WRKY6 in Arabidopsis thalianaMarkus G. Stetter0Martin Benz1Uwe Ludewig2Institute of Crop Science, Nutritional Crop Physiology, University of Hohenheim, Stuttgart, GermanyInstitute of Crop Science, Nutritional Crop Physiology, University of Hohenheim, Stuttgart, GermanyInstitute of Crop Science, Nutritional Crop Physiology, University of Hohenheim, Stuttgart, GermanyRoot hairs are unicellular elongations of certain rhizodermal cells that improve the uptake of sparingly soluble and immobile soil nutrients. Among different Arabidopsis thaliana genotypes, root hair density, length and the local acclimation to low inorganic phosphate (Pi) differs considerably, when analyzed on split agar plates. Here, genome-wide association fine mapping identified significant single nucleotide polymorphisms associated with the increased root hair density in the absence of local phosphate on chromosome 1. A loss-of-functionmutant of the candidate transcription factor gene WRKY6, which is involved in the acclimation of plants to low phosphorus, had increased root hair density. This is partially explained by a reduced cortical cell diameter in wrky6-3, reducing the rhizodermal cell numbers adjacent to the cortical cells. As a consequence, rhizodermal cells in positions that are in contact with two cortical cells are found more often, leading to higher hair density. Distinct cortical cell diameters and epidermal cell lengths distinguish other Arabidopsis accessions with distinct root hair density and −Pi response from diploid Col-0, while tetraploid Col-0 had generally larger root cell sizes, which explain longer hairs. A distinct radial root morphology within Arabidopsis accessions and wrky6-3explains some, but not all, differences in the root hair acclimation to –Pi.https://peerj.com/articles/2891.pdfArabidopsisRoot hairEcotypesGenome wide associationMixed modelNutrition |
| spellingShingle | Markus G. Stetter Martin Benz Uwe Ludewig Increased root hair density by loss of WRKY6 in Arabidopsis thaliana PeerJ Arabidopsis Root hair Ecotypes Genome wide association Mixed model Nutrition |
| title | Increased root hair density by loss of WRKY6 in Arabidopsis thaliana |
| title_full | Increased root hair density by loss of WRKY6 in Arabidopsis thaliana |
| title_fullStr | Increased root hair density by loss of WRKY6 in Arabidopsis thaliana |
| title_full_unstemmed | Increased root hair density by loss of WRKY6 in Arabidopsis thaliana |
| title_short | Increased root hair density by loss of WRKY6 in Arabidopsis thaliana |
| title_sort | increased root hair density by loss of wrky6 in arabidopsis thaliana |
| topic | Arabidopsis Root hair Ecotypes Genome wide association Mixed model Nutrition |
| url | https://peerj.com/articles/2891.pdf |
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