Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage
Cyanobacteria are gaining considerable interest as a method of supporting the long-term presence of humans on the Moon and settlements on Mars due to their ability to produce oxygen and their potential as bio-factories for space biotechnology/synthetic biology and other applications. Since many unkn...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1150224/full |
| _version_ | 1797825431476371456 |
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| author | Claudia Fagliarone Claudia Mosca Giorgia Di Stefano Giorgia Di Stefano Stefan Leuko Ralf Moeller Ralf Moeller Elke Rabbow Petra Rettberg Daniela Billi |
| author_facet | Claudia Fagliarone Claudia Mosca Giorgia Di Stefano Giorgia Di Stefano Stefan Leuko Ralf Moeller Ralf Moeller Elke Rabbow Petra Rettberg Daniela Billi |
| author_sort | Claudia Fagliarone |
| collection | DOAJ |
| description | Cyanobacteria are gaining considerable interest as a method of supporting the long-term presence of humans on the Moon and settlements on Mars due to their ability to produce oxygen and their potential as bio-factories for space biotechnology/synthetic biology and other applications. Since many unknowns remain in our knowledge to bridge the gap and move cyanobacterial bioprocesses from Earth to space, we investigated cell division resumption on the rehydration of dried Chroococcidiopsis sp. CCMEE 029 accumulated DNA damage while exposed to space vacuum, Mars-like conditions, and Fe-ion radiation. Upon rehydration, the monitoring of the ftsZ gene showed that cell division was arrested until DNA damage was repaired, which took 48 h under laboratory conditions. During the recovery, a progressive DNA repair lasting 48 h of rehydration was revealed by PCR-stop assay. This was followed by overexpression of the ftsZ gene, ranging from 7.5- to 9-fold compared to the non-hydrated samples. Knowing the time required for DNA repair and cell division resumption is mandatory for deep-space experiments that are designed to unravel the effects of reduced/microgravity on this process. It is also necessary to meet mission requirements for dried-sample implementation and real-time monitoring upon recovery. Future experiments as part of the lunar exploration mission Artemis and the lunar gateway station will undoubtedly help to move cyanobacterial bioprocesses beyond low Earth orbit. From an astrobiological perspective, these experiments will further our understanding of microbial responses to deep-space conditions. |
| first_indexed | 2024-03-13T10:53:55Z |
| format | Article |
| id | doaj.art-0148e4d74bb0446a8f5535d06d987e2f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T10:53:55Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0148e4d74bb0446a8f5535d06d987e2f2023-05-17T05:33:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11502241150224Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damageClaudia Fagliarone0Claudia Mosca1Giorgia Di Stefano2Giorgia Di Stefano3Stefan Leuko4Ralf Moeller5Ralf Moeller6Elke Rabbow7Petra Rettberg8Daniela Billi9Department of Biology, University of Rome Tor Vergata, Rome, ItalyDepartment of Biology, University of Rome Tor Vergata, Rome, ItalyDepartment of Biology, University of Rome Tor Vergata, Rome, ItalyPhD Program in Cellular and Molecular Biology, Department of Biology, University of Rome Tor Vergata, Rome, ItalyAerospace Microbiology Research Group, Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, GermanyAerospace Microbiology Research Group, Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, GermanyDepartment of Natural Sciences, University of Applied Sciences Bonn-Rhein-Sieg (BRSU), Rheinbach, GermanyAstrobiology Research Group, Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, GermanyAstrobiology Research Group, Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, GermanyDepartment of Biology, University of Rome Tor Vergata, Rome, ItalyCyanobacteria are gaining considerable interest as a method of supporting the long-term presence of humans on the Moon and settlements on Mars due to their ability to produce oxygen and their potential as bio-factories for space biotechnology/synthetic biology and other applications. Since many unknowns remain in our knowledge to bridge the gap and move cyanobacterial bioprocesses from Earth to space, we investigated cell division resumption on the rehydration of dried Chroococcidiopsis sp. CCMEE 029 accumulated DNA damage while exposed to space vacuum, Mars-like conditions, and Fe-ion radiation. Upon rehydration, the monitoring of the ftsZ gene showed that cell division was arrested until DNA damage was repaired, which took 48 h under laboratory conditions. During the recovery, a progressive DNA repair lasting 48 h of rehydration was revealed by PCR-stop assay. This was followed by overexpression of the ftsZ gene, ranging from 7.5- to 9-fold compared to the non-hydrated samples. Knowing the time required for DNA repair and cell division resumption is mandatory for deep-space experiments that are designed to unravel the effects of reduced/microgravity on this process. It is also necessary to meet mission requirements for dried-sample implementation and real-time monitoring upon recovery. Future experiments as part of the lunar exploration mission Artemis and the lunar gateway station will undoubtedly help to move cyanobacterial bioprocesses beyond low Earth orbit. From an astrobiological perspective, these experiments will further our understanding of microbial responses to deep-space conditions.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1150224/fullouter spaceDNA damagecell divisionFe-ion radiationdesert cyanobacteria |
| spellingShingle | Claudia Fagliarone Claudia Mosca Giorgia Di Stefano Giorgia Di Stefano Stefan Leuko Ralf Moeller Ralf Moeller Elke Rabbow Petra Rettberg Daniela Billi Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage Frontiers in Microbiology outer space DNA damage cell division Fe-ion radiation desert cyanobacteria |
| title | Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage |
| title_full | Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage |
| title_fullStr | Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage |
| title_full_unstemmed | Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage |
| title_short | Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage |
| title_sort | enabling deep space experimentations on cyanobacteria by monitoring cell division resumption in dried chroococcidiopsis sp 029 with accumulated dna damage |
| topic | outer space DNA damage cell division Fe-ion radiation desert cyanobacteria |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1150224/full |
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