APPLICATION OF MONOCLONAL ANTIBODY-BASED LATEX AGGLUTINATION TEST FOR DETECTION AND IDENTIFICATION OF THE AGENT OF MELIOIDOSIS IN CLINICAL AND ENVIRONMENTAL OBJECTS
The review summarizes the basic information on the development and diagnostic capabilities of the latex agglutination test (LAT), used for detection and subsequent identification of melioidosis pathogen. According to the published literature, the use of melioidosis monoclonal antibodies of various e...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2018-02-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.elpub.ru/jour/article/view/252 |
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| author | N. P. Khrapova L. K. Merinova T. V. Zamarina D. M. Frolov T. V. Senina I. I. Korsakova |
| author_facet | N. P. Khrapova L. K. Merinova T. V. Zamarina D. M. Frolov T. V. Senina I. I. Korsakova |
| author_sort | N. P. Khrapova |
| collection | DOAJ |
| description | The review summarizes the basic information on the development and diagnostic capabilities of the latex agglutination test (LAT), used for detection and subsequent identification of melioidosis pathogen. According to the published literature, the use of melioidosis monoclonal antibodies of various epitope direction for coat the latex beads (suspension carrier), the main detection ingredient of this reaction, contributes to an increase in the diagnostic capabilities of this method: its sensitivity and specificity, which has been repeatedly confirmed by specialists working both in endemic zone distribution of Burkholderia pseudomallei and outside these territories. As most authors of the publications noted, after introduction of this reaction into practical work of profile laboratories, low-cost commercial products (test set of reagents for latex agglutination reaction) can find wide application both in stationary and mobile laboratories. The undoubted merits of the method are its simplicity, clarity, suitability for working with various samples from objects of the external environment and biological material, as well as obtained evidence ofthe suitability of LAT to ensure effective differentiation of B. pseudomallei from avirulent related bacteria and detection of the causative agent in the early stages of the disease for relatively short intervals, which makes it a promising method for diagnosing melioidosis, a potentially fatal infection requiring an early onset appropriate antibiotic therapy. |
| first_indexed | 2024-04-13T02:33:35Z |
| format | Article |
| id | doaj.art-01491465ae484ca1b8d82bfd1d5d0e76 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-13T02:33:35Z |
| publishDate | 2018-02-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-01491465ae484ca1b8d82bfd1d5d0e762022-12-22T03:06:28ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132018-02-0101849210.36233/0372-9311-2018-1-84-92252APPLICATION OF MONOCLONAL ANTIBODY-BASED LATEX AGGLUTINATION TEST FOR DETECTION AND IDENTIFICATION OF THE AGENT OF MELIOIDOSIS IN CLINICAL AND ENVIRONMENTAL OBJECTSN. P. Khrapova0L. K. Merinova1T. V. Zamarina2D. M. Frolov3T. V. Senina4I. I. Korsakova5Volgograd Research Institute for Plague ControlVolgograd Research Institute for Plague ControlVolgograd Research Institute for Plague ControlVolgograd Research Institute for Plague ControlVolgograd Research Institute for Plague ControlVolgograd Research Institute for Plague ControlThe review summarizes the basic information on the development and diagnostic capabilities of the latex agglutination test (LAT), used for detection and subsequent identification of melioidosis pathogen. According to the published literature, the use of melioidosis monoclonal antibodies of various epitope direction for coat the latex beads (suspension carrier), the main detection ingredient of this reaction, contributes to an increase in the diagnostic capabilities of this method: its sensitivity and specificity, which has been repeatedly confirmed by specialists working both in endemic zone distribution of Burkholderia pseudomallei and outside these territories. As most authors of the publications noted, after introduction of this reaction into practical work of profile laboratories, low-cost commercial products (test set of reagents for latex agglutination reaction) can find wide application both in stationary and mobile laboratories. The undoubted merits of the method are its simplicity, clarity, suitability for working with various samples from objects of the external environment and biological material, as well as obtained evidence ofthe suitability of LAT to ensure effective differentiation of B. pseudomallei from avirulent related bacteria and detection of the causative agent in the early stages of the disease for relatively short intervals, which makes it a promising method for diagnosing melioidosis, a potentially fatal infection requiring an early onset appropriate antibiotic therapy.https://microbiol.elpub.ru/jour/article/view/252burkholderia pseudomalleilatex agglutinationburkholderia pseudomalleimonoclonal antibody |
| spellingShingle | N. P. Khrapova L. K. Merinova T. V. Zamarina D. M. Frolov T. V. Senina I. I. Korsakova APPLICATION OF MONOCLONAL ANTIBODY-BASED LATEX AGGLUTINATION TEST FOR DETECTION AND IDENTIFICATION OF THE AGENT OF MELIOIDOSIS IN CLINICAL AND ENVIRONMENTAL OBJECTS Журнал микробиологии, эпидемиологии и иммунобиологии burkholderia pseudomallei latex agglutination burkholderia pseudomallei monoclonal antibody |
| title | APPLICATION OF MONOCLONAL ANTIBODY-BASED LATEX AGGLUTINATION TEST FOR DETECTION AND IDENTIFICATION OF THE AGENT OF MELIOIDOSIS IN CLINICAL AND ENVIRONMENTAL OBJECTS |
| title_full | APPLICATION OF MONOCLONAL ANTIBODY-BASED LATEX AGGLUTINATION TEST FOR DETECTION AND IDENTIFICATION OF THE AGENT OF MELIOIDOSIS IN CLINICAL AND ENVIRONMENTAL OBJECTS |
| title_fullStr | APPLICATION OF MONOCLONAL ANTIBODY-BASED LATEX AGGLUTINATION TEST FOR DETECTION AND IDENTIFICATION OF THE AGENT OF MELIOIDOSIS IN CLINICAL AND ENVIRONMENTAL OBJECTS |
| title_full_unstemmed | APPLICATION OF MONOCLONAL ANTIBODY-BASED LATEX AGGLUTINATION TEST FOR DETECTION AND IDENTIFICATION OF THE AGENT OF MELIOIDOSIS IN CLINICAL AND ENVIRONMENTAL OBJECTS |
| title_short | APPLICATION OF MONOCLONAL ANTIBODY-BASED LATEX AGGLUTINATION TEST FOR DETECTION AND IDENTIFICATION OF THE AGENT OF MELIOIDOSIS IN CLINICAL AND ENVIRONMENTAL OBJECTS |
| title_sort | application of monoclonal antibody based latex agglutination test for detection and identification of the agent of melioidosis in clinical and environmental objects |
| topic | burkholderia pseudomallei latex agglutination burkholderia pseudomallei monoclonal antibody |
| url | https://microbiol.elpub.ru/jour/article/view/252 |
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