Arabidopsis thaliana GTS1 transcripts are activated by yeast extract
WD40 repeat-containing proteins participate in DNA-protein and protein-protein interactions and positively regulate plant stress responses. GTS1, known as a WD40 repeat-containing protein, works as a scaffold protein and is important in ribosome biogenesis and also biomass accumulation. In this stud...
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| Format: | Article |
| Language: | English |
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University of Belgrade
2021-10-01
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| Series: | Botanica Serbica |
| Subjects: | |
| Online Access: | https://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2021_45_2_800_full.pdf |
| _version_ | 1830198930078433280 |
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| author | Burcu Arıkan Aslı Semercі Özgür Çakır Neslihan Turgut Kara |
| author_facet | Burcu Arıkan Aslı Semercі Özgür Çakır Neslihan Turgut Kara |
| author_sort | Burcu Arıkan |
| collection | DOAJ |
| description | WD40 repeat-containing proteins participate in DNA-protein and protein-protein interactions and positively regulate plant stress responses. GTS1, known as a WD40 repeat-containing protein, works as a scaffold protein and is important in ribosome biogenesis and also biomass accumulation. In this study, we evaluated the GIGANTUS1 (GTS1) gene expression in response to biotic and abiotic stress factors in Arabidopsis thaliana plants. In addition, we grew and characterized A. thaliana gts1 mutant (T-DNA SALK_010647) in order to observe the effects of its absence on plants. According to our results, 100-200 mM abscisic acid (ABA) and 100-200 mM sodium chloride (NaCl) treatment did not cause any changes in GTS1 gene expression, while only 6 h of 1 g/l and 2 g/l yeast extract (YE) treatment negatively affected GTS1 expression in 10-day-old plant explants. After 10 and 30 days of YE treatment, GTS1 gene expression was upregulated, and as a consequence plant growth efficiency was reduced. We thus concluded that through the downregulation of GTS1 transcripts, we could obtain better growth and/or higher biomass, which seems to be a good option for agricultural recruitments. |
| first_indexed | 2024-12-18T02:03:17Z |
| format | Article |
| id | doaj.art-01498636899c41608346244f2689f770 |
| institution | Directory Open Access Journal |
| issn | 1821-2158 1821-2638 |
| language | English |
| last_indexed | 2024-12-18T02:03:17Z |
| publishDate | 2021-10-01 |
| publisher | University of Belgrade |
| record_format | Article |
| series | Botanica Serbica |
| spelling | doaj.art-01498636899c41608346244f2689f7702022-12-21T21:24:40ZengUniversity of BelgradeBotanica Serbica1821-21581821-26382021-10-0145219520110.2298/BOTSERB2102195AArabidopsis thaliana GTS1 transcripts are activated by yeast extractBurcu Arıkan0Aslı Semercі1Özgür Çakır2Neslihan Turgut Kara3Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, 34134, Istanbul, TurkeyIstanbul University, Faculty of Science, Department of Molecular Biology and Genetics, 34134, Istanbul, TurkeyIstanbul University, Faculty of Science, Department of Molecular Biology and Genetics, 34134, Istanbul, TurkeyIstanbul University, Faculty of Science, Department of Molecular Biology and Genetics, 34134, Istanbul, TurkeyWD40 repeat-containing proteins participate in DNA-protein and protein-protein interactions and positively regulate plant stress responses. GTS1, known as a WD40 repeat-containing protein, works as a scaffold protein and is important in ribosome biogenesis and also biomass accumulation. In this study, we evaluated the GIGANTUS1 (GTS1) gene expression in response to biotic and abiotic stress factors in Arabidopsis thaliana plants. In addition, we grew and characterized A. thaliana gts1 mutant (T-DNA SALK_010647) in order to observe the effects of its absence on plants. According to our results, 100-200 mM abscisic acid (ABA) and 100-200 mM sodium chloride (NaCl) treatment did not cause any changes in GTS1 gene expression, while only 6 h of 1 g/l and 2 g/l yeast extract (YE) treatment negatively affected GTS1 expression in 10-day-old plant explants. After 10 and 30 days of YE treatment, GTS1 gene expression was upregulated, and as a consequence plant growth efficiency was reduced. We thus concluded that through the downregulation of GTS1 transcripts, we could obtain better growth and/or higher biomass, which seems to be a good option for agricultural recruitments.https://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2021_45_2_800_full.pdfgigantus1arabidopsis thalianayeast extractbiotic stresswd40 repeat |
| spellingShingle | Burcu Arıkan Aslı Semercі Özgür Çakır Neslihan Turgut Kara Arabidopsis thaliana GTS1 transcripts are activated by yeast extract Botanica Serbica gigantus1 arabidopsis thaliana yeast extract biotic stress wd40 repeat |
| title | Arabidopsis thaliana GTS1 transcripts are activated by yeast extract |
| title_full | Arabidopsis thaliana GTS1 transcripts are activated by yeast extract |
| title_fullStr | Arabidopsis thaliana GTS1 transcripts are activated by yeast extract |
| title_full_unstemmed | Arabidopsis thaliana GTS1 transcripts are activated by yeast extract |
| title_short | Arabidopsis thaliana GTS1 transcripts are activated by yeast extract |
| title_sort | arabidopsis thaliana gts1 transcripts are activated by yeast extract |
| topic | gigantus1 arabidopsis thaliana yeast extract biotic stress wd40 repeat |
| url | https://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2021_45_2_800_full.pdf |
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