Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria
Abstract Glycogen serves as a metabolic sink in cyanobacteria. Glycogen deficiency causes the extracellular release of distinctive metabolites such as pyruvate and 2-oxoglutarate upon nitrogen depletion; however, the mechanism has not been fully elucidated. This study aimed to elucidate the mechanis...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-05929-9 |
| _version_ | 1797273503079071744 |
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| author | Yuichi Kato Ryota Hidese Mami Matsuda Ryudo Ohbayashi Hiroki Ashida Akihiko Kondo Tomohisa Hasunuma |
| author_facet | Yuichi Kato Ryota Hidese Mami Matsuda Ryudo Ohbayashi Hiroki Ashida Akihiko Kondo Tomohisa Hasunuma |
| author_sort | Yuichi Kato |
| collection | DOAJ |
| description | Abstract Glycogen serves as a metabolic sink in cyanobacteria. Glycogen deficiency causes the extracellular release of distinctive metabolites such as pyruvate and 2-oxoglutarate upon nitrogen depletion; however, the mechanism has not been fully elucidated. This study aimed to elucidate the mechanism of carbon partitioning in glycogen-deficient cyanobacteria. Extracellular and intracellular metabolites in a glycogen-deficient ΔglgC mutant of Synechococcus elongatus PCC 7942 were comprehensively analyzed. In the presence of a nitrogen source, the ΔglgC mutant released extracellular glutamate rather than pyruvate and 2-oxoglutarate, whereas its intracellular glutamate level was lower than that in the wild-type strain. The de novo synthesis of glutamate increased in the ΔglgC mutant, suggesting that glycogen deficiency enhanced carbon partitioning into glutamate and extracellular excretion through an unidentified transport system. This study proposes a model in which glutamate serves as the prime extracellular metabolic sink alternative to glycogen when nitrogen is available. |
| first_indexed | 2024-03-07T14:45:18Z |
| format | Article |
| id | doaj.art-014f918e275f452594e9c39949cd3c15 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-07T14:45:18Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-014f918e275f452594e9c39949cd3c152024-03-05T19:59:51ZengNature PortfolioCommunications Biology2399-36422024-02-01711910.1038/s42003-024-05929-9Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteriaYuichi Kato0Ryota Hidese1Mami Matsuda2Ryudo Ohbayashi3Hiroki Ashida4Akihiko Kondo5Tomohisa Hasunuma6Engineering Biology Research Center, Kobe UniversityEngineering Biology Research Center, Kobe UniversityEngineering Biology Research Center, Kobe UniversityDepartment of Biological Science, Faculty of Sciences, Shizuoka UniversityGraduate School of Human Development and Environment, Kobe UniversityEngineering Biology Research Center, Kobe UniversityEngineering Biology Research Center, Kobe UniversityAbstract Glycogen serves as a metabolic sink in cyanobacteria. Glycogen deficiency causes the extracellular release of distinctive metabolites such as pyruvate and 2-oxoglutarate upon nitrogen depletion; however, the mechanism has not been fully elucidated. This study aimed to elucidate the mechanism of carbon partitioning in glycogen-deficient cyanobacteria. Extracellular and intracellular metabolites in a glycogen-deficient ΔglgC mutant of Synechococcus elongatus PCC 7942 were comprehensively analyzed. In the presence of a nitrogen source, the ΔglgC mutant released extracellular glutamate rather than pyruvate and 2-oxoglutarate, whereas its intracellular glutamate level was lower than that in the wild-type strain. The de novo synthesis of glutamate increased in the ΔglgC mutant, suggesting that glycogen deficiency enhanced carbon partitioning into glutamate and extracellular excretion through an unidentified transport system. This study proposes a model in which glutamate serves as the prime extracellular metabolic sink alternative to glycogen when nitrogen is available.https://doi.org/10.1038/s42003-024-05929-9 |
| spellingShingle | Yuichi Kato Ryota Hidese Mami Matsuda Ryudo Ohbayashi Hiroki Ashida Akihiko Kondo Tomohisa Hasunuma Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria Communications Biology |
| title | Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria |
| title_full | Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria |
| title_fullStr | Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria |
| title_full_unstemmed | Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria |
| title_short | Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria |
| title_sort | glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria |
| url | https://doi.org/10.1038/s42003-024-05929-9 |
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