(+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle Arrest
Extracts of Chinese herbs have been demonstrated to inhibit oxidative stress in vitro. In this study, we investigated the mechanism of (+)-catechin, isolated from green tea, which preserved murine microglia N9 cells from an oxidative agent tert-butylhydroperoxide (tBHP)-induced cell death. (+)-Catec...
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| Format: | Article |
| Language: | English |
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Elsevier
2005-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319322108 |
| _version_ | 1828413643082956800 |
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| author | Qing Huang Li-Jun Wu Shin-ichi Tashiro Hui-Yuan Gao Satoshi Onodera Takashi Ikejima |
| author_facet | Qing Huang Li-Jun Wu Shin-ichi Tashiro Hui-Yuan Gao Satoshi Onodera Takashi Ikejima |
| author_sort | Qing Huang |
| collection | DOAJ |
| description | Extracts of Chinese herbs have been demonstrated to inhibit oxidative stress in vitro. In this study, we investigated the mechanism of (+)-catechin, isolated from green tea, which preserved murine microglia N9 cells from an oxidative agent tert-butylhydroperoxide (tBHP)-induced cell death. (+)-Catechin augmented the cell survival ratio after exposure to tBHP. Protective action of this drug was more efficacious than that of N-acetylcysteine, which is a putative antioxidant. DNA damage, detected by the Comet assay, was diminished with treatment of the drug. Results of flow cytometric analysis showed that the amount of intracellular • OH was decreased, and the cell cycle arrest was reversed by down-regulation of p53 phosphorylation after treatment with (+)-catechin. The reduced p53 activity followed the impairment of NF-κB translocation to the nuclear region. Then the phosphorylation of extracellular signal regulated protein kinase, a cell survival facilitative signal, was upregulated at the later stage. Taken together, (+)-catechin inhibited tBHP-induced translocation of NF-κB to improve cellular survival. Keywords:: (+)-catechin, DNA damage, cell cycle arrest, NF-κB, p53 |
| first_indexed | 2024-12-10T13:12:35Z |
| format | Article |
| id | doaj.art-01520be565414664a49ea3498891bd2b |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-10T13:12:35Z |
| publishDate | 2005-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-01520be565414664a49ea3498891bd2b2022-12-22T01:47:38ZengElsevierJournal of Pharmacological Sciences1347-86132005-01-019811624(+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle ArrestQing Huang0Li-Jun Wu1Shin-ichi Tashiro2Hui-Yuan Gao3Satoshi Onodera4Takashi Ikejima5China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China; Department of Phytochemistry, Shenyang Pharmaceutical University, Shenyang 110016, ChinaDepartment of Phytochemistry, Shenyang Pharmaceutical University, Shenyang 110016, ChinaDepartment of Clinical and Biomedical Sciences, Showa Pharmaceutical University, Tokyo 194-8543, JapanDepartment of Phytochemistry, Shenyang Pharmaceutical University, Shenyang 110016, ChinaDepartment of Clinical and Biomedical Sciences, Showa Pharmaceutical University, Tokyo 194-8543, JapanChina-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China; Corresponding author. FAX: +86-24-23844463 E-mail: ikejimat@vip.sina.comExtracts of Chinese herbs have been demonstrated to inhibit oxidative stress in vitro. In this study, we investigated the mechanism of (+)-catechin, isolated from green tea, which preserved murine microglia N9 cells from an oxidative agent tert-butylhydroperoxide (tBHP)-induced cell death. (+)-Catechin augmented the cell survival ratio after exposure to tBHP. Protective action of this drug was more efficacious than that of N-acetylcysteine, which is a putative antioxidant. DNA damage, detected by the Comet assay, was diminished with treatment of the drug. Results of flow cytometric analysis showed that the amount of intracellular • OH was decreased, and the cell cycle arrest was reversed by down-regulation of p53 phosphorylation after treatment with (+)-catechin. The reduced p53 activity followed the impairment of NF-κB translocation to the nuclear region. Then the phosphorylation of extracellular signal regulated protein kinase, a cell survival facilitative signal, was upregulated at the later stage. Taken together, (+)-catechin inhibited tBHP-induced translocation of NF-κB to improve cellular survival. Keywords:: (+)-catechin, DNA damage, cell cycle arrest, NF-κB, p53http://www.sciencedirect.com/science/article/pii/S1347861319322108 |
| spellingShingle | Qing Huang Li-Jun Wu Shin-ichi Tashiro Hui-Yuan Gao Satoshi Onodera Takashi Ikejima (+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle Arrest Journal of Pharmacological Sciences |
| title | (+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle Arrest |
| title_full | (+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle Arrest |
| title_fullStr | (+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle Arrest |
| title_full_unstemmed | (+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle Arrest |
| title_short | (+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle Arrest |
| title_sort | catechin an ingredient of green tea protects murine microglia from oxidative stress induced dna damage and cell cycle arrest |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319322108 |
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