Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice.
Bone marrow cells (BMC) migrate to the injured liver after transplantation, contributing to regeneration through multiple pathways, but mechanisms involved are unclear. This work aimed to study BMC migration, characterize cytokine profile, cell populations and proliferation in mice with liver fibros...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5703547?pdf=render |
| _version_ | 1811225097408610304 |
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| author | Daphne Pinheiro Luana Leirós Juliana Barbosa Torreão Dáu Ana Carolina Stumbo Alessandra Alves Thole Erika Afonso Costa Cortez Carlos Alberto Mandarim-de-Lacerda Lais de Carvalho Simone Nunes de Carvalho |
| author_facet | Daphne Pinheiro Luana Leirós Juliana Barbosa Torreão Dáu Ana Carolina Stumbo Alessandra Alves Thole Erika Afonso Costa Cortez Carlos Alberto Mandarim-de-Lacerda Lais de Carvalho Simone Nunes de Carvalho |
| author_sort | Daphne Pinheiro |
| collection | DOAJ |
| description | Bone marrow cells (BMC) migrate to the injured liver after transplantation, contributing to regeneration through multiple pathways, but mechanisms involved are unclear. This work aimed to study BMC migration, characterize cytokine profile, cell populations and proliferation in mice with liver fibrosis transplanted with GFP+ BMC. Confocal microscopy analysis showed GFP+ BMC near regions expressing HGF and SDF-1 in the fibrotic liver. Impaired liver cell proliferation in fibrotic groups was restored after BMC transplantation. Regarding total cell populations, there was a significant reduction in CD68+ cells and increased Ly6G+ cells in transplanted fibrotic group. BMC contributed to the total populations of CD144, CD11b and Ly6G cells in the fibrotic liver, related to an increment of anti-fibrotic cytokines (IL-10, IL-13, IFN-γ and HGF) and reduction of pro-inflammatory cytokines (IL-17A and IL-6). Therefore, HGF and SDF-1 may represent important chemoattractants for transplanted BMC in the injured liver, where these cells can give rise to populations of extrahepatic macrophages, neutrophils and endothelial progenitor cells that can interact synergistically with other liver cells towards the modulation of an anti-fibrotic cytokine profile promoting the onset of liver regeneration. |
| first_indexed | 2024-04-12T09:01:07Z |
| format | Article |
| id | doaj.art-0152239033eb4f018a51c9b3952f176d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T09:01:07Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0152239033eb4f018a51c9b3952f176d2022-12-22T03:39:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018797010.1371/journal.pone.0187970Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice.Daphne PinheiroLuana LeirósJuliana Barbosa Torreão DáuAna Carolina StumboAlessandra Alves TholeErika Afonso Costa CortezCarlos Alberto Mandarim-de-LacerdaLais de CarvalhoSimone Nunes de CarvalhoBone marrow cells (BMC) migrate to the injured liver after transplantation, contributing to regeneration through multiple pathways, but mechanisms involved are unclear. This work aimed to study BMC migration, characterize cytokine profile, cell populations and proliferation in mice with liver fibrosis transplanted with GFP+ BMC. Confocal microscopy analysis showed GFP+ BMC near regions expressing HGF and SDF-1 in the fibrotic liver. Impaired liver cell proliferation in fibrotic groups was restored after BMC transplantation. Regarding total cell populations, there was a significant reduction in CD68+ cells and increased Ly6G+ cells in transplanted fibrotic group. BMC contributed to the total populations of CD144, CD11b and Ly6G cells in the fibrotic liver, related to an increment of anti-fibrotic cytokines (IL-10, IL-13, IFN-γ and HGF) and reduction of pro-inflammatory cytokines (IL-17A and IL-6). Therefore, HGF and SDF-1 may represent important chemoattractants for transplanted BMC in the injured liver, where these cells can give rise to populations of extrahepatic macrophages, neutrophils and endothelial progenitor cells that can interact synergistically with other liver cells towards the modulation of an anti-fibrotic cytokine profile promoting the onset of liver regeneration.http://europepmc.org/articles/PMC5703547?pdf=render |
| spellingShingle | Daphne Pinheiro Luana Leirós Juliana Barbosa Torreão Dáu Ana Carolina Stumbo Alessandra Alves Thole Erika Afonso Costa Cortez Carlos Alberto Mandarim-de-Lacerda Lais de Carvalho Simone Nunes de Carvalho Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice. PLoS ONE |
| title | Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice. |
| title_full | Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice. |
| title_fullStr | Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice. |
| title_full_unstemmed | Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice. |
| title_short | Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice. |
| title_sort | cytokines hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice |
| url | http://europepmc.org/articles/PMC5703547?pdf=render |
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