Molecular characterization of mecA gene in Methicillin-Resistant Staphylococcus aureus
Background & objectives: It is clear now that methicillin-resistant Staphylococcus aureus (MRSA) strains considered as one of the most bacteria responsible for different diseases among humans and animals. The present study aimed to detect the molecular profile of methicillin-resistant S. aureus...
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| Format: | Article |
| Language: | English |
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University of Thi-Qar
2019-04-01
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| Series: | مجلة علوم ذي قار |
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| Online Access: | http://jsci.utq.edu.iq/index.php/main/article/view/50 |
| _version_ | 1797404153697271808 |
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| author | Saad Salman Hamim |
| author_facet | Saad Salman Hamim |
| author_sort | Saad Salman Hamim |
| collection | DOAJ |
| description | Background & objectives: It is clear now that methicillin-resistant Staphylococcus aureus (MRSA) strains considered as one of the most bacteria responsible for different diseases among humans and animals. The present study aimed to detect the molecular profile of methicillin-resistant S. aureus isolated from skin abscess patients in Nassyriah City, Southern Iraq.
Methods: During the period of from June 2014 to February 2015, 120 S. aureus were isolated from abscess patient in two governmental hospitals, and subjected to conventional Polymerase Chain Reaction which used for the amplification of 310 bp mecA gene. Three PCR products of mecA were named primarily (THQR1, THQR2, and THQR3) were selected and subjected to partial DNA sequencing for the mecA gene to follow up their possible relationship between these local isolates and what recorded globally in Genbank. Results: Only 64 S. aureus isolates were diagnosed phenotypically as MRSA (53.33%), and 88/120 (73.33%) of S. aureus were positive for the targeted gene. The Three PCR products of mecA were registered in Genbank under the official accession numbers of (KY468502, KY468503 and KY468504, respectively). The constructed phylogenetic tree showed that S. aureus KY468502 and KY468504 were highly relative to each other in comparison with S. aureus KY468503 that revealed a close relatedness to S. aureus TN/CN/1/12, mecA gene beta-lactam, and mecA gene isolated in USA.
Interpretation & conclusions: The present study results confirmed the importance of mecA gene in MRSA detection and highlighted the increasing manner of its prevalence in Iraq, furthermore, the importance of molecular techniques as an epidemiological tool. |
| first_indexed | 2024-03-09T02:50:48Z |
| format | Article |
| id | doaj.art-0152d5110df0466f807d98984f723cef |
| institution | Directory Open Access Journal |
| issn | 1991-8690 2709-0256 |
| language | English |
| last_indexed | 2024-03-09T02:50:48Z |
| publishDate | 2019-04-01 |
| publisher | University of Thi-Qar |
| record_format | Article |
| series | مجلة علوم ذي قار |
| spelling | doaj.art-0152d5110df0466f807d98984f723cef2023-12-05T09:58:23ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562019-04-0161Molecular characterization of mecA gene in Methicillin-Resistant Staphylococcus aureusSaad Salman Hamim0Department of Pathological Analysis/College of Science/University of Thi-Qar- IraqBackground & objectives: It is clear now that methicillin-resistant Staphylococcus aureus (MRSA) strains considered as one of the most bacteria responsible for different diseases among humans and animals. The present study aimed to detect the molecular profile of methicillin-resistant S. aureus isolated from skin abscess patients in Nassyriah City, Southern Iraq. Methods: During the period of from June 2014 to February 2015, 120 S. aureus were isolated from abscess patient in two governmental hospitals, and subjected to conventional Polymerase Chain Reaction which used for the amplification of 310 bp mecA gene. Three PCR products of mecA were named primarily (THQR1, THQR2, and THQR3) were selected and subjected to partial DNA sequencing for the mecA gene to follow up their possible relationship between these local isolates and what recorded globally in Genbank. Results: Only 64 S. aureus isolates were diagnosed phenotypically as MRSA (53.33%), and 88/120 (73.33%) of S. aureus were positive for the targeted gene. The Three PCR products of mecA were registered in Genbank under the official accession numbers of (KY468502, KY468503 and KY468504, respectively). The constructed phylogenetic tree showed that S. aureus KY468502 and KY468504 were highly relative to each other in comparison with S. aureus KY468503 that revealed a close relatedness to S. aureus TN/CN/1/12, mecA gene beta-lactam, and mecA gene isolated in USA. Interpretation & conclusions: The present study results confirmed the importance of mecA gene in MRSA detection and highlighted the increasing manner of its prevalence in Iraq, furthermore, the importance of molecular techniques as an epidemiological tool.http://jsci.utq.edu.iq/index.php/main/article/view/50Gene sequencingMethicillin-resistantStaphylococcus aureusPhylogenetic treemecA gene of S. aureus |
| spellingShingle | Saad Salman Hamim Molecular characterization of mecA gene in Methicillin-Resistant Staphylococcus aureus مجلة علوم ذي قار Gene sequencing Methicillin-resistant Staphylococcus aureus Phylogenetic tree mecA gene of S. aureus |
| title | Molecular characterization of mecA gene in Methicillin-Resistant Staphylococcus aureus |
| title_full | Molecular characterization of mecA gene in Methicillin-Resistant Staphylococcus aureus |
| title_fullStr | Molecular characterization of mecA gene in Methicillin-Resistant Staphylococcus aureus |
| title_full_unstemmed | Molecular characterization of mecA gene in Methicillin-Resistant Staphylococcus aureus |
| title_short | Molecular characterization of mecA gene in Methicillin-Resistant Staphylococcus aureus |
| title_sort | molecular characterization of meca gene in methicillin resistant staphylococcus aureus |
| topic | Gene sequencing Methicillin-resistant Staphylococcus aureus Phylogenetic tree mecA gene of S. aureus |
| url | http://jsci.utq.edu.iq/index.php/main/article/view/50 |
| work_keys_str_mv | AT saadsalmanhamim molecularcharacterizationofmecageneinmethicillinresistantstaphylococcusaureus |