CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE
Results of cloning X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate and analysis of their nucleotide sequences are presented. Obtained clones were added to the previously constructed clone library comprising clones of 8 genes of Krasnodar 06/12 isolate. Clones containin...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-07-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/354 |
| _version_ | 1797224915374440448 |
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| author | Ali Mazloum N. G. Zinyakov A. S. Igolkin N. N. Vlasova |
| author_facet | Ali Mazloum N. G. Zinyakov A. S. Igolkin N. N. Vlasova |
| author_sort | Ali Mazloum |
| collection | DOAJ |
| description | Results of cloning X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate and analysis of their nucleotide sequences are presented. Obtained clones were added to the previously constructed clone library comprising clones of 8 genes of Krasnodar 06/12 isolate. Clones containing X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate will be used for recombinant protein obtaining and testing for their effect on in vitro virus reproduction and their role in the virus infectivity, level of clinical manifestations and virulence. Prokaryotic vector, pJET1.2/ blunt, was used. Thus, the clone library available at the FGBI “ARRIAH” Reference Laboratory for African swine fever was supplemented by pJET1.2-X69R, pJET1.2-A179L, pJET1.2-E248R, pJET1.2-I215L and pJET1.2-DP96R plasmid constructions containing 5 genes of ASF virus Krasnodar 07/17 isolate. Proportion of cloned virus genes was 3.01% of Krasnodar 07/17 isolate genome, hence, total amount of the clone library has reached 7.82%. |
| first_indexed | 2024-03-09T02:49:50Z |
| format | Article |
| id | doaj.art-0153394e0caa43d7a9615d0952a77fe1 |
| institution | Directory Open Access Journal |
| issn | 2304-196X 2658-6959 |
| language | English |
| last_indexed | 2024-04-24T14:00:42Z |
| publishDate | 2018-07-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj.art-0153394e0caa43d7a9615d0952a77fe12024-04-03T12:48:48ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-07-010281210.29326/2304-196X-2018-2-25-8-12390CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCEAli Mazloum0N. G. Zinyakov1A. S. Igolkin2N. N. Vlasova3FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirResults of cloning X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate and analysis of their nucleotide sequences are presented. Obtained clones were added to the previously constructed clone library comprising clones of 8 genes of Krasnodar 06/12 isolate. Clones containing X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate will be used for recombinant protein obtaining and testing for their effect on in vitro virus reproduction and their role in the virus infectivity, level of clinical manifestations and virulence. Prokaryotic vector, pJET1.2/ blunt, was used. Thus, the clone library available at the FGBI “ARRIAH” Reference Laboratory for African swine fever was supplemented by pJET1.2-X69R, pJET1.2-A179L, pJET1.2-E248R, pJET1.2-I215L and pJET1.2-DP96R plasmid constructions containing 5 genes of ASF virus Krasnodar 07/17 isolate. Proportion of cloned virus genes was 3.01% of Krasnodar 07/17 isolate genome, hence, total amount of the clone library has reached 7.82%.https://veterinary.arriah.ru/jour/article/view/354african swine fever (asf)transmembrane proteinsproteins responsible for asf virus virulencesequencinggene clone library |
| spellingShingle | Ali Mazloum N. G. Zinyakov A. S. Igolkin N. N. Vlasova CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE Ветеринария сегодня african swine fever (asf) transmembrane proteins proteins responsible for asf virus virulence sequencing gene clone library |
| title | CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE |
| title_full | CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE |
| title_fullStr | CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE |
| title_full_unstemmed | CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE |
| title_short | CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE |
| title_sort | cloning of genes encoding transmembrane proteins and proteins responsible for african swine fever virus virulence |
| topic | african swine fever (asf) transmembrane proteins proteins responsible for asf virus virulence sequencing gene clone library |
| url | https://veterinary.arriah.ru/jour/article/view/354 |
| work_keys_str_mv | AT alimazloum cloningofgenesencodingtransmembraneproteinsandproteinsresponsibleforafricanswinefevervirusvirulence AT ngzinyakov cloningofgenesencodingtransmembraneproteinsandproteinsresponsibleforafricanswinefevervirusvirulence AT asigolkin cloningofgenesencodingtransmembraneproteinsandproteinsresponsibleforafricanswinefevervirusvirulence AT nnvlasova cloningofgenesencodingtransmembraneproteinsandproteinsresponsibleforafricanswinefevervirusvirulence |