Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis
Superoxide dismutase (SOD) is a very important reactive oxygen species (ROS)-scavenging enzyme. In this study, the functions of a Cu/Zn SOD gene (SaCu/Zn SOD), from Sedum alfredii, a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized. The expression of SaCu/Zn SOD was...
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01010/full |
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| author | Zhen Li Zhen Li Xiaojiao Han Xiaojiao Han Xixi Song Xixi Song Yunxing Zhang Yunxing Zhang Jing Jiang Jing Jiang Qiang Han Qiang Han Mingying Liu Mingying Liu Guirong Qiao Guirong Qiao Renying Zhuo Renying Zhuo |
| author_facet | Zhen Li Zhen Li Xiaojiao Han Xiaojiao Han Xixi Song Xixi Song Yunxing Zhang Yunxing Zhang Jing Jiang Jing Jiang Qiang Han Qiang Han Mingying Liu Mingying Liu Guirong Qiao Guirong Qiao Renying Zhuo Renying Zhuo |
| author_sort | Zhen Li |
| collection | DOAJ |
| description | Superoxide dismutase (SOD) is a very important reactive oxygen species (ROS)-scavenging enzyme. In this study, the functions of a Cu/Zn SOD gene (SaCu/Zn SOD), from Sedum alfredii, a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized. The expression of SaCu/Zn SOD was induced by Cd stress. Compared with wild-type (WT) plants, overexpression of SaCu/Zn SOD gene in transgenic Arabidopsis plants enhanced the antioxidative defense capacity, including SOD and peroxidase activities. Additionally, it reduced the damage associated with the overproduction of hydrogen peroxide (H2O2) and superoxide radicals (O2•-). The influence of Cd stress on ion flux across the root surface showed that overexpressing SaCu/Zn SOD in transgenic Arabidopsis plants has greater Cd uptake capacity existed in roots. A co-expression network based on microarray data showed possible oxidative regulation in Arabidopsis after Cd-induced oxidative stress, suggesting that SaCu/Zn SOD may participate in this network and enhance ROS-scavenging capability under Cd stress. Taken together, these results suggest that overexpressing SaCu/Zn SOD increased oxidative stress resistance in transgenic Arabidopsis and provide useful information for understanding the role of SaCu/Zn SOD in response to abiotic stress. |
| first_indexed | 2024-12-12T08:32:50Z |
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| id | doaj.art-01552345dd3a4dd19dd1e15f57e4b860 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T08:32:50Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-01552345dd3a4dd19dd1e15f57e4b8602022-12-22T00:31:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-06-01810.3389/fpls.2017.01010246576Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic ArabidopsisZhen Li0Zhen Li1Xiaojiao Han2Xiaojiao Han3Xixi Song4Xixi Song5Yunxing Zhang6Yunxing Zhang7Jing Jiang8Jing Jiang9Qiang Han10Qiang Han11Mingying Liu12Mingying Liu13Guirong Qiao14Guirong Qiao15Renying Zhuo16Renying Zhuo17State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, ChinaState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, ChinaSuperoxide dismutase (SOD) is a very important reactive oxygen species (ROS)-scavenging enzyme. In this study, the functions of a Cu/Zn SOD gene (SaCu/Zn SOD), from Sedum alfredii, a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized. The expression of SaCu/Zn SOD was induced by Cd stress. Compared with wild-type (WT) plants, overexpression of SaCu/Zn SOD gene in transgenic Arabidopsis plants enhanced the antioxidative defense capacity, including SOD and peroxidase activities. Additionally, it reduced the damage associated with the overproduction of hydrogen peroxide (H2O2) and superoxide radicals (O2•-). The influence of Cd stress on ion flux across the root surface showed that overexpressing SaCu/Zn SOD in transgenic Arabidopsis plants has greater Cd uptake capacity existed in roots. A co-expression network based on microarray data showed possible oxidative regulation in Arabidopsis after Cd-induced oxidative stress, suggesting that SaCu/Zn SOD may participate in this network and enhance ROS-scavenging capability under Cd stress. Taken together, these results suggest that overexpressing SaCu/Zn SOD increased oxidative stress resistance in transgenic Arabidopsis and provide useful information for understanding the role of SaCu/Zn SOD in response to abiotic stress.http://journal.frontiersin.org/article/10.3389/fpls.2017.01010/fullCd stressco-expression networkCu/Zn SODoxidative stressSedum alfredii |
| spellingShingle | Zhen Li Zhen Li Xiaojiao Han Xiaojiao Han Xixi Song Xixi Song Yunxing Zhang Yunxing Zhang Jing Jiang Jing Jiang Qiang Han Qiang Han Mingying Liu Mingying Liu Guirong Qiao Guirong Qiao Renying Zhuo Renying Zhuo Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis Frontiers in Plant Science Cd stress co-expression network Cu/Zn SOD oxidative stress Sedum alfredii |
| title | Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis |
| title_full | Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis |
| title_fullStr | Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis |
| title_full_unstemmed | Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis |
| title_short | Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis |
| title_sort | overexpressing the sedum alfredii cu zn superoxide dismutase increased resistance to oxidative stress in transgenic arabidopsis |
| topic | Cd stress co-expression network Cu/Zn SOD oxidative stress Sedum alfredii |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01010/full |
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