Argonaute protein-based nucleic acid detection technology
It is vital to diagnose pathogens quickly and effectively in the research and treatment of disease. Argonaute (Ago) proteins are recently discovered nucleases with nucleic acid shearing activity that exhibit specific recognition properties beyond CRISPR–Cas nucleases, which are highly researched but...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1255716/full |
| _version_ | 1797689845443723264 |
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| author | Zhiyun Wu Li Yu Weifeng Shi Jinhong Ma |
| author_facet | Zhiyun Wu Li Yu Weifeng Shi Jinhong Ma |
| author_sort | Zhiyun Wu |
| collection | DOAJ |
| description | It is vital to diagnose pathogens quickly and effectively in the research and treatment of disease. Argonaute (Ago) proteins are recently discovered nucleases with nucleic acid shearing activity that exhibit specific recognition properties beyond CRISPR–Cas nucleases, which are highly researched but restricted PAM sequence recognition. Therefore, research on Ago protein-mediated nucleic acid detection technology has attracted significant attention from researchers in recent years. Using Ago proteins in developing nucleic acid detection platforms can enable efficient, convenient, and rapid nucleic acid detection and pathogen diagnosis, which is of great importance for human life and health and technological development. In this article, we introduce the structure and function of Argonaute proteins and discuss the latest advances in their use in nucleic acid detection. |
| first_indexed | 2024-03-12T01:50:52Z |
| format | Article |
| id | doaj.art-01554a2659d6498d81b42ec4995baeaf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T01:50:52Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-01554a2659d6498d81b42ec4995baeaf2023-09-08T12:15:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12557161255716Argonaute protein-based nucleic acid detection technologyZhiyun Wu0Li Yu1Weifeng Shi2Jinhong Ma3Department of Clinical Laboratory, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, ChinaJiangsu Key Laboratory of Medical Science and Laboratory Medicine, Institute of Stem Cell, School of Medicine, Jiangsu University, Zhenjiang, ChinaDepartment of Clinical Laboratory, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, ChinaDepartment of Clinical Laboratory, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, ChinaIt is vital to diagnose pathogens quickly and effectively in the research and treatment of disease. Argonaute (Ago) proteins are recently discovered nucleases with nucleic acid shearing activity that exhibit specific recognition properties beyond CRISPR–Cas nucleases, which are highly researched but restricted PAM sequence recognition. Therefore, research on Ago protein-mediated nucleic acid detection technology has attracted significant attention from researchers in recent years. Using Ago proteins in developing nucleic acid detection platforms can enable efficient, convenient, and rapid nucleic acid detection and pathogen diagnosis, which is of great importance for human life and health and technological development. In this article, we introduce the structure and function of Argonaute proteins and discuss the latest advances in their use in nucleic acid detection.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1255716/fullnucleic acid detection technologypathogen diagnosisArgonautenucleasesbiosensor |
| spellingShingle | Zhiyun Wu Li Yu Weifeng Shi Jinhong Ma Argonaute protein-based nucleic acid detection technology Frontiers in Microbiology nucleic acid detection technology pathogen diagnosis Argonaute nucleases biosensor |
| title | Argonaute protein-based nucleic acid detection technology |
| title_full | Argonaute protein-based nucleic acid detection technology |
| title_fullStr | Argonaute protein-based nucleic acid detection technology |
| title_full_unstemmed | Argonaute protein-based nucleic acid detection technology |
| title_short | Argonaute protein-based nucleic acid detection technology |
| title_sort | argonaute protein based nucleic acid detection technology |
| topic | nucleic acid detection technology pathogen diagnosis Argonaute nucleases biosensor |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1255716/full |
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