In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot Tips
Synthetic seed technology and cold storage methods provide genetic uniformity, paving the way for pest- and disease-free plants that are easy to handle. The aim of this study was to develop protocols for the cold storage of nonencapsulated and alginate-capsulated explants of <i>Cannabis sativa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Biology and Life Sciences Forum |
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| Online Access: | https://www.mdpi.com/2673-9976/27/1/15 |
| _version_ | 1827306802027429888 |
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| author | Mariola Dreger Aleksandra Deja Milena Szalata Ryszard Słomski |
| author_facet | Mariola Dreger Aleksandra Deja Milena Szalata Ryszard Słomski |
| author_sort | Mariola Dreger |
| collection | DOAJ |
| description | Synthetic seed technology and cold storage methods provide genetic uniformity, paving the way for pest- and disease-free plants that are easy to handle. The aim of this study was to develop protocols for the cold storage of nonencapsulated and alginate-capsulated explants of <i>Cannabis sativa</i> L. Axillary shoot tips derived from in vitro-grown plants that were used as explants and stored for up to 9 months at 4 °C in the dark. Somatic seeds were produced in 3% sodium alginate and Murashige and Skoog (MS) medium salt and stored for up to 3 months. After 6 months of cold storage, the highest regrowth of 45% was recorded for the nonencapsulated explants. The recovery of somatic seeds was 90% under the same storage condition after 3 months. Well-developed, regenerated plants from encapsulated explants successfully acclimatized. |
| first_indexed | 2024-04-24T18:29:41Z |
| format | Article |
| id | doaj.art-01580d6a704f4faab7393145cfc3837c |
| institution | Directory Open Access Journal |
| issn | 2673-9976 |
| language | English |
| last_indexed | 2024-04-24T18:29:41Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology and Life Sciences Forum |
| spelling | doaj.art-01580d6a704f4faab7393145cfc3837c2024-03-27T13:28:22ZengMDPI AGBiology and Life Sciences Forum2673-99762023-10-012711510.3390/IECAG2023-15146In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot TipsMariola Dreger0Aleksandra Deja1Milena Szalata2Ryszard Słomski3Department of Biotechnology, Institute of Natural Fibres & Medicinal Plants—National Research Institute, Wojska Polskiego 71b, 60-630 Poznan, PolandDepartment of Biotechnology, Institute of Natural Fibres & Medicinal Plants—National Research Institute, Wojska Polskiego 71b, 60-630 Poznan, PolandDepartment of Biotechnology, Institute of Natural Fibres & Medicinal Plants—National Research Institute, Wojska Polskiego 71b, 60-630 Poznan, PolandDepartment of Biotechnology, Institute of Natural Fibres & Medicinal Plants—National Research Institute, Wojska Polskiego 71b, 60-630 Poznan, PolandSynthetic seed technology and cold storage methods provide genetic uniformity, paving the way for pest- and disease-free plants that are easy to handle. The aim of this study was to develop protocols for the cold storage of nonencapsulated and alginate-capsulated explants of <i>Cannabis sativa</i> L. Axillary shoot tips derived from in vitro-grown plants that were used as explants and stored for up to 9 months at 4 °C in the dark. Somatic seeds were produced in 3% sodium alginate and Murashige and Skoog (MS) medium salt and stored for up to 3 months. After 6 months of cold storage, the highest regrowth of 45% was recorded for the nonencapsulated explants. The recovery of somatic seeds was 90% under the same storage condition after 3 months. Well-developed, regenerated plants from encapsulated explants successfully acclimatized.https://www.mdpi.com/2673-9976/27/1/15synthetic seedscold storagehemp<i>Cannabis sativa</i> L.in vitro culture |
| spellingShingle | Mariola Dreger Aleksandra Deja Milena Szalata Ryszard Słomski In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot Tips Biology and Life Sciences Forum synthetic seeds cold storage hemp <i>Cannabis sativa</i> L. in vitro culture |
| title | In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot Tips |
| title_full | In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot Tips |
| title_fullStr | In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot Tips |
| title_full_unstemmed | In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot Tips |
| title_short | In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot Tips |
| title_sort | in vitro preservation of somatic seeds and nonencapsulated hemp shoot tips |
| topic | synthetic seeds cold storage hemp <i>Cannabis sativa</i> L. in vitro culture |
| url | https://www.mdpi.com/2673-9976/27/1/15 |
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