Utilization of Corncob Hydrolysate Enables 2,3-Butanediol Production in Enterobacter cholerae
2,3-Butanediol (2,3-BDO) is an important industrial diol that could function in various fields. Currently, there are many substrates used for 2,3-BDO biosynthesis, but studies using green carbon sources such as corncob hydrolysate as a substrate are lacking. As a widely distributed waste lignocellul...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2022-11-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/22046 |
| _version_ | 1827916052098449408 |
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| author | Lizhou Ma Jieming Wang Rui Zhi Lucian A. Lucia Guohui Li Yu Deng |
| author_facet | Lizhou Ma Jieming Wang Rui Zhi Lucian A. Lucia Guohui Li Yu Deng |
| author_sort | Lizhou Ma |
| collection | DOAJ |
| description | 2,3-Butanediol (2,3-BDO) is an important industrial diol that could function in various fields. Currently, there are many substrates used for 2,3-BDO biosynthesis, but studies using green carbon sources such as corncob hydrolysate as a substrate are lacking. As a widely distributed waste lignocellulose-derived substrate, corncob hydrolysate is nutrient-rich and cost-effective. The present study evaluated 2,3-BDO production via an Enterobacter cholerae strain using corncob hydrolysate as carbon source. Chemical component analysis showed that concentrated corncob hydrolysate contained 233 g/L total sugar and showed no inhibitory effect, but it was beneficial for 2,3-BDO synthesis. Optimization experiments for fermentation resulted in a titer of 47.23 g/L 2,3-BDO with a yield of 0.30 g/g and a productivity rate of 0.66 g/L•h. This study is expected to provide insights for large-scale bioproduction of bulk chemicals utilizing corncob hydrolysates. |
| first_indexed | 2024-03-13T03:08:24Z |
| format | Article |
| id | doaj.art-01598a9afdc74b9e91844dd2a4a890a1 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-03-13T03:08:24Z |
| publishDate | 2022-11-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-01598a9afdc74b9e91844dd2a4a890a12023-06-26T18:27:19ZengNorth Carolina State UniversityBioResources1930-21262022-11-011745820583048Utilization of Corncob Hydrolysate Enables 2,3-Butanediol Production in Enterobacter choleraeLizhou Ma0Jieming Wang1Rui Zhi2Lucian A. Lucia3Guohui Li4Yu Deng5Jiangnan UniversityJiangnan UniversityJiangnan UniversityNorth Carolina State UniversityJiangnan UniversityJiangnan University2,3-Butanediol (2,3-BDO) is an important industrial diol that could function in various fields. Currently, there are many substrates used for 2,3-BDO biosynthesis, but studies using green carbon sources such as corncob hydrolysate as a substrate are lacking. As a widely distributed waste lignocellulose-derived substrate, corncob hydrolysate is nutrient-rich and cost-effective. The present study evaluated 2,3-BDO production via an Enterobacter cholerae strain using corncob hydrolysate as carbon source. Chemical component analysis showed that concentrated corncob hydrolysate contained 233 g/L total sugar and showed no inhibitory effect, but it was beneficial for 2,3-BDO synthesis. Optimization experiments for fermentation resulted in a titer of 47.23 g/L 2,3-BDO with a yield of 0.30 g/g and a productivity rate of 0.66 g/L•h. This study is expected to provide insights for large-scale bioproduction of bulk chemicals utilizing corncob hydrolysates.https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/220462,3-butanediolenterobacter choleraecorncob hydrolysatetwo-stage fermentationlignocellulose |
| spellingShingle | Lizhou Ma Jieming Wang Rui Zhi Lucian A. Lucia Guohui Li Yu Deng Utilization of Corncob Hydrolysate Enables 2,3-Butanediol Production in Enterobacter cholerae BioResources 2,3-butanediol enterobacter cholerae corncob hydrolysate two-stage fermentation lignocellulose |
| title | Utilization of Corncob Hydrolysate Enables 2,3-Butanediol Production in Enterobacter cholerae |
| title_full | Utilization of Corncob Hydrolysate Enables 2,3-Butanediol Production in Enterobacter cholerae |
| title_fullStr | Utilization of Corncob Hydrolysate Enables 2,3-Butanediol Production in Enterobacter cholerae |
| title_full_unstemmed | Utilization of Corncob Hydrolysate Enables 2,3-Butanediol Production in Enterobacter cholerae |
| title_short | Utilization of Corncob Hydrolysate Enables 2,3-Butanediol Production in Enterobacter cholerae |
| title_sort | utilization of corncob hydrolysate enables 2 3 butanediol production in enterobacter cholerae |
| topic | 2,3-butanediol enterobacter cholerae corncob hydrolysate two-stage fermentation lignocellulose |
| url | https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/22046 |
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