Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties
Abstract Development of coating technologies for electrochemical sensors that consistently exhibit antifouling activities in diverse and complex biological environments over extended time is vital for effective medical devices and diagnostics. Here, we describe a micrometer-thick, porous nanocomposi...
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| Format: | Article |
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Nature Portfolio
2024-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-44822-1 |
| _version_ | 1797273927786954752 |
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| author | Jeong-Chan Lee Su Yeong Kim Jayeon Song Hyowon Jang Min Kim Hanul Kim Siyoung Q. Choi Sunjoo Kim Pawan Jolly Taejoon Kang Steve Park Donald E. Ingber |
| author_facet | Jeong-Chan Lee Su Yeong Kim Jayeon Song Hyowon Jang Min Kim Hanul Kim Siyoung Q. Choi Sunjoo Kim Pawan Jolly Taejoon Kang Steve Park Donald E. Ingber |
| author_sort | Jeong-Chan Lee |
| collection | DOAJ |
| description | Abstract Development of coating technologies for electrochemical sensors that consistently exhibit antifouling activities in diverse and complex biological environments over extended time is vital for effective medical devices and diagnostics. Here, we describe a micrometer-thick, porous nanocomposite coating with both antifouling and electroconducting properties that enhances the sensitivity of electrochemical sensors. Nozzle printing of oil-in-water emulsion is used to create a 1 micrometer thick coating composed of cross-linked albumin with interconnected pores and gold nanowires. The layer resists biofouling and maintains rapid electron transfer kinetics for over one month when exposed directly to complex biological fluids, including serum and nasopharyngeal secretions. Compared to a thinner (nanometer thick) antifouling coating made with drop casting or a spin coating of the same thickness, the thick porous nanocomposite sensor exhibits sensitivities that are enhanced by 3.75- to 17-fold when three different target biomolecules are tested. As a result, emulsion-coated, multiplexed electrochemical sensors can carry out simultaneous detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid, antigen, and host antibody in clinical specimens with high sensitivity and specificity. This thick porous emulsion coating technology holds promise in addressing hurdles currently restricting the application of electrochemical sensors for point-of-care diagnostics, implantable devices, and other healthcare monitoring systems. |
| first_indexed | 2024-03-07T14:51:08Z |
| format | Article |
| id | doaj.art-015a6a6345f84aa09864c957fcc97a30 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-07T14:51:08Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-015a6a6345f84aa09864c957fcc97a302024-03-05T19:38:40ZengNature PortfolioNature Communications2041-17232024-02-0115111410.1038/s41467-024-44822-1Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting propertiesJeong-Chan Lee0Su Yeong Kim1Jayeon Song2Hyowon Jang3Min Kim4Hanul Kim5Siyoung Q. Choi6Sunjoo Kim7Pawan Jolly8Taejoon Kang9Steve Park10Donald E. Ingber11Wyss Institute for Biologically Inspired Engineering, Harvard UniversityDepartment of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST)Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST)Department of Chemical and Biomolecular Engineering, KAISTDepartment of Chemical and Biomolecular Engineering, KAISTDepartment of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of MedicineWyss Institute for Biologically Inspired Engineering, Harvard UniversityBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST)Wyss Institute for Biologically Inspired Engineering, Harvard UniversityAbstract Development of coating technologies for electrochemical sensors that consistently exhibit antifouling activities in diverse and complex biological environments over extended time is vital for effective medical devices and diagnostics. Here, we describe a micrometer-thick, porous nanocomposite coating with both antifouling and electroconducting properties that enhances the sensitivity of electrochemical sensors. Nozzle printing of oil-in-water emulsion is used to create a 1 micrometer thick coating composed of cross-linked albumin with interconnected pores and gold nanowires. The layer resists biofouling and maintains rapid electron transfer kinetics for over one month when exposed directly to complex biological fluids, including serum and nasopharyngeal secretions. Compared to a thinner (nanometer thick) antifouling coating made with drop casting or a spin coating of the same thickness, the thick porous nanocomposite sensor exhibits sensitivities that are enhanced by 3.75- to 17-fold when three different target biomolecules are tested. As a result, emulsion-coated, multiplexed electrochemical sensors can carry out simultaneous detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid, antigen, and host antibody in clinical specimens with high sensitivity and specificity. This thick porous emulsion coating technology holds promise in addressing hurdles currently restricting the application of electrochemical sensors for point-of-care diagnostics, implantable devices, and other healthcare monitoring systems.https://doi.org/10.1038/s41467-024-44822-1 |
| spellingShingle | Jeong-Chan Lee Su Yeong Kim Jayeon Song Hyowon Jang Min Kim Hanul Kim Siyoung Q. Choi Sunjoo Kim Pawan Jolly Taejoon Kang Steve Park Donald E. Ingber Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties Nature Communications |
| title | Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties |
| title_full | Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties |
| title_fullStr | Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties |
| title_full_unstemmed | Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties |
| title_short | Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties |
| title_sort | micrometer thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties |
| url | https://doi.org/10.1038/s41467-024-44822-1 |
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