Ischemic preconditioning affects phosphosites and accentuates myocardial stunning while reducing infarction size in rats
Background and aimsIschemic preconditioning (IPC), i.e., brief periods of ischemia, protect the heart from subsequent prolonged ischemic injury, and reduces infarction size. Myocardial stunning refers to transient loss of contractility in the heart after myocardial ischemia that recovers without per...
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2024.1376367/full |
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| author | Ahmed Elmahdy Ahmed Elmahdy Aaron Shekka Espinosa Aaron Shekka Espinosa Yalda Kakaei Yalda Kakaei Tetiana Pylova Tetiana Pylova Abhishek Jha Abhishek Jha Ermir Zulfaj Maryna Krasnikova Maryna Krasnikova Amin Al-Awar Amin Al-Awar Zahra Sheybani Zahra Sheybani Valentyna Sevastianova Valentyna Sevastianova Evelin Berger Amirali Nejat Linnea Molander Linnea Molander Erik Axel Andersson Erik Axel Andersson Elmir Omerovic Elmir Omerovic Shafaat Hussain Shafaat Hussain Björn Redfors Björn Redfors Björn Redfors |
| author_facet | Ahmed Elmahdy Ahmed Elmahdy Aaron Shekka Espinosa Aaron Shekka Espinosa Yalda Kakaei Yalda Kakaei Tetiana Pylova Tetiana Pylova Abhishek Jha Abhishek Jha Ermir Zulfaj Maryna Krasnikova Maryna Krasnikova Amin Al-Awar Amin Al-Awar Zahra Sheybani Zahra Sheybani Valentyna Sevastianova Valentyna Sevastianova Evelin Berger Amirali Nejat Linnea Molander Linnea Molander Erik Axel Andersson Erik Axel Andersson Elmir Omerovic Elmir Omerovic Shafaat Hussain Shafaat Hussain Björn Redfors Björn Redfors Björn Redfors |
| author_sort | Ahmed Elmahdy |
| collection | DOAJ |
| description | Background and aimsIschemic preconditioning (IPC), i.e., brief periods of ischemia, protect the heart from subsequent prolonged ischemic injury, and reduces infarction size. Myocardial stunning refers to transient loss of contractility in the heart after myocardial ischemia that recovers without permanent damage. The relationship between IPC and myocardial stunning remains incompletely understood. This study aimed primarily to examine the effects of IPC on the relationship between ischemia duration, stunning, and infarct size in an ischemia-reperfusion injury model. Secondarily, this study aimed to examine to which extent the phosphoproteomic changes induced by IPC relate to myocardial contractile function.Methods and resultsRats were subjected to different durations of left anterior descending artery (LAD) occlusion, with or without preceding IPC. Echocardiograms were acquired to assess cardiac contraction in the affected myocardial segment. Infarction size was evaluated using triphenyl tetrazolium chloride staining. Phosphoproteomic analysis was performed in heart tissue from preconditioned and non-preconditioned animals. In contrast to rats without IPC, reversible akinesia was observed in a majority of the rats that were subjected to IPC and subsequently exposed to ischemia of 13.5 or 15 min of ischemia. Phosphoproteomic analysis revealed significant differential regulation of 786 phosphopeptides between IPC and non-IPC groups, with significant associations with the sarcomere, Z-disc, and actin binding.ConclusionIPC induces changes in phosphosites of proteins involved in myocardial contraction; and both accentuates post-ischemic myocardial stunning and reduces infarct size. |
| first_indexed | 2024-04-24T23:46:10Z |
| format | Article |
| id | doaj.art-015b6376ee204eecbd09715653d0c609 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-24T23:46:10Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-015b6376ee204eecbd09715653d0c6092024-03-15T04:50:05ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2024-03-011110.3389/fcvm.2024.13763671376367Ischemic preconditioning affects phosphosites and accentuates myocardial stunning while reducing infarction size in ratsAhmed Elmahdy0Ahmed Elmahdy1Aaron Shekka Espinosa2Aaron Shekka Espinosa3Yalda Kakaei4Yalda Kakaei5Tetiana Pylova6Tetiana Pylova7Abhishek Jha8Abhishek Jha9Ermir Zulfaj10Maryna Krasnikova11Maryna Krasnikova12Amin Al-Awar13Amin Al-Awar14Zahra Sheybani15Zahra Sheybani16Valentyna Sevastianova17Valentyna Sevastianova18Evelin Berger19Amirali Nejat20Linnea Molander21Linnea Molander22Erik Axel Andersson23Erik Axel Andersson24Elmir Omerovic25Elmir Omerovic26Shafaat Hussain27Shafaat Hussain28Björn Redfors29Björn Redfors30Björn Redfors31Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenProteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDepartment of Cardiology, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenWallenberg Centre for Molecular and Translational Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Cardiology, Sahlgrenska University Hospital, Gothenburg, SwedenBackground and aimsIschemic preconditioning (IPC), i.e., brief periods of ischemia, protect the heart from subsequent prolonged ischemic injury, and reduces infarction size. Myocardial stunning refers to transient loss of contractility in the heart after myocardial ischemia that recovers without permanent damage. The relationship between IPC and myocardial stunning remains incompletely understood. This study aimed primarily to examine the effects of IPC on the relationship between ischemia duration, stunning, and infarct size in an ischemia-reperfusion injury model. Secondarily, this study aimed to examine to which extent the phosphoproteomic changes induced by IPC relate to myocardial contractile function.Methods and resultsRats were subjected to different durations of left anterior descending artery (LAD) occlusion, with or without preceding IPC. Echocardiograms were acquired to assess cardiac contraction in the affected myocardial segment. Infarction size was evaluated using triphenyl tetrazolium chloride staining. Phosphoproteomic analysis was performed in heart tissue from preconditioned and non-preconditioned animals. In contrast to rats without IPC, reversible akinesia was observed in a majority of the rats that were subjected to IPC and subsequently exposed to ischemia of 13.5 or 15 min of ischemia. Phosphoproteomic analysis revealed significant differential regulation of 786 phosphopeptides between IPC and non-IPC groups, with significant associations with the sarcomere, Z-disc, and actin binding.ConclusionIPC induces changes in phosphosites of proteins involved in myocardial contraction; and both accentuates post-ischemic myocardial stunning and reduces infarct size.https://www.frontiersin.org/articles/10.3389/fcvm.2024.1376367/fullmyocardial infarctionmyocardial stunningischemic preconditioningechocardiographyspeckle tracking analysisphosphoproteomics |
| spellingShingle | Ahmed Elmahdy Ahmed Elmahdy Aaron Shekka Espinosa Aaron Shekka Espinosa Yalda Kakaei Yalda Kakaei Tetiana Pylova Tetiana Pylova Abhishek Jha Abhishek Jha Ermir Zulfaj Maryna Krasnikova Maryna Krasnikova Amin Al-Awar Amin Al-Awar Zahra Sheybani Zahra Sheybani Valentyna Sevastianova Valentyna Sevastianova Evelin Berger Amirali Nejat Linnea Molander Linnea Molander Erik Axel Andersson Erik Axel Andersson Elmir Omerovic Elmir Omerovic Shafaat Hussain Shafaat Hussain Björn Redfors Björn Redfors Björn Redfors Ischemic preconditioning affects phosphosites and accentuates myocardial stunning while reducing infarction size in rats Frontiers in Cardiovascular Medicine myocardial infarction myocardial stunning ischemic preconditioning echocardiography speckle tracking analysis phosphoproteomics |
| title | Ischemic preconditioning affects phosphosites and accentuates myocardial stunning while reducing infarction size in rats |
| title_full | Ischemic preconditioning affects phosphosites and accentuates myocardial stunning while reducing infarction size in rats |
| title_fullStr | Ischemic preconditioning affects phosphosites and accentuates myocardial stunning while reducing infarction size in rats |
| title_full_unstemmed | Ischemic preconditioning affects phosphosites and accentuates myocardial stunning while reducing infarction size in rats |
| title_short | Ischemic preconditioning affects phosphosites and accentuates myocardial stunning while reducing infarction size in rats |
| title_sort | ischemic preconditioning affects phosphosites and accentuates myocardial stunning while reducing infarction size in rats |
| topic | myocardial infarction myocardial stunning ischemic preconditioning echocardiography speckle tracking analysis phosphoproteomics |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2024.1376367/full |
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